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The application of spectral karyotyping in leukemia

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Chinese Journal of Clinical Oncology

Abstract

Spectral karyotyping (SKY) is a novel cytogenetic technique, which has been developed to unambiguously display and identify all 24 human chromosomes at one time without previous knowledge of any abnormalities involved. SKY can discern aberrations that fail to be easily detected by conventional banding techniques and by fluorescent in situ hybridization (FISH). Therefore SKY is highly accurate, highly sensitive, and highly prognostic. In this report the featurese and application of SKY in studies of leukemia are reviewed.

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References

  1. Speicher MR, Gwyn Ballard S, Ward DC. Karyotyping human chromosomes by combinatorial multi-fluor FISH. Nat Genet. 1996;12:368–375.

    Article  PubMed  CAS  Google Scholar 

  2. Schock E, du Manoir S, Veldman T, et al. Multicolor spectral karyotyping of human chromosomes. Science. 1996;273:494–497.

    Article  Google Scholar 

  3. Fan YS, Siu VM, Jung JH, et al. Sensitivity of multiple color spectral karyotyping in detecteding small interchromosomal rearrangements. Genet Test. 2000;4:9–14.

    Article  PubMed  CAS  Google Scholar 

  4. Schock E, Veldman T, Padilla-Nash H, et al. Spectral karyotyping refines cytogenetic diagnostics of constitutional chromosomal abnormalities. Hum Genet. 1997;101:255–262.

    Article  Google Scholar 

  5. Tchinda J, Neumann TE, Volpert S, et al. Characterization of Chromosomal Rearrangements in Hematological Diseases Using Spectral Karyotyping. Diagn Mol Pathol. 2004;13:190–195.

    Article  PubMed  Google Scholar 

  6. Kakazu N, Taniwaki M, Horiike S, et al. Combined spectral karyotyping and DAPI banding analysis of chromosome abnormalities in myelodysplastic syndrome. Genes Chromosomes Cancer. 1999;26:336–345.

    Article  PubMed  CAS  Google Scholar 

  7. Ye CJ, Lu W, Liu G, et al. The combination of SKY and specific loci detection with FISH or immunostaining. Cytogenet Cell Genet. 2001;93:195–202.

    Article  PubMed  CAS  Google Scholar 

  8. Nordgren A, Heyman M, Sahlen S, et al. Spectral karyotyping and interphase FISH reveal abnormalities not detected by conventional G-banding. Implications for treatment stratification of childhood acute lymphoblastic leukaemia: detailed analysis of 70 cases. Eur J Haematol. 2002;68:31–41.

    Article  PubMed  CAS  Google Scholar 

  9. Zhang FF, Murata-Collins JL, Gaytan P, et al. Twenty-four-color spectral karyotyping reveals chromosome aberrations in cytogenetically normal acute myeloid leukemia. Genes Chromosomes Cancer. 2000;28:318–328.

    Article  PubMed  CAS  Google Scholar 

  10. Odero MD, Carlson KM, Calasanz MJ, et al. Further characterization of complex chromosomal rearrangements in myeloid malignancies: spectral karyotyping adds precision in defining abnormalities associated with poor prognosis. Leukemia. 2001;15:1133–1136.

    Article  PubMed  CAS  Google Scholar 

  11. Veldman T, Vignon C, Schrock E, et al. Hidden chromosome abnormalities in haematological malignancies detected by multi-color spectral karyotyping. Nat Genet. 1997;15:406–410.

    Article  PubMed  CAS  Google Scholar 

  12. Mrozek K, Tanner SM, Heinonen K, et al. Molecular cytogenetic characterization of the KG-1 and KG-1a acute myeloid leukemia cell lines by use of spectral karyotyping and fluorescence in situ hybridization. Genes Chromosomes Cancer. 2003;38:249–252.

    Article  PubMed  CAS  Google Scholar 

  13. Pelz AF, Weilepp G, Wieacker PF. Re-analysis of the cell line NALM-1 karyotype by GTG-banding, spectral karyotyping, and whole chromosome painting. Cancer Genet Cytogenet. 2005;156:59–61.

    Article  PubMed  CAS  Google Scholar 

  14. Chen IM, Whalen M, Bankhurst A, et al. A new human natural killer leukemia cell line, IMC-1. A complex chromosomal rearrangement defined by spectral karyotyping: functional and cytogenetic characterization. Leuk Res. 2004;28:275–284.

    Article  PubMed  CAS  Google Scholar 

  15. Ohsaka A, Hisa T. Spectral karyotyping refined the identification of a der(Y)t(Y;1)(q11.1 or.2;q12) in the blast cells of a patient with atypical chronic myeloid leukemia. Acta Haematol. 2002;107:224–229.

    Article  PubMed  Google Scholar 

  16. Hilgenfeld E, Padilla-Nash H, McNeil N, et al. Spectral karyotyping and fluorescence in situ hybridization detect novel chromosomal aberrations, a recurring involvement of chromosome 21 and amplification of the MYC oncogene in acute myeloid leukemia M2. Br J Haematol. 2001;113:305–317.

    Article  PubMed  CAS  Google Scholar 

  17. Mohr B, Bornhauser M, Thiede C, et al. Comparison of spectral karyotyping and conventional cytogenetics in 39 patients with acute myeloid leukemia and myelodysplastic syndrome. Leukemia. 2000;14:1031–1038.

    Article  PubMed  CAS  Google Scholar 

  18. Gitte BK, Eigil Kjeldsen, Acute Leukemia cytogenetics: an evaluation of combining G-band karyotyping with multicolor spectral karyotyping. Cancer Genetics and Cytogenetics. 2001;124:7–11.

    Article  Google Scholar 

  19. Lu XY, Harris CP, Cooley L, et al. The utility of spectral karyotyping in the cytogenetic analysis of newly diagnosed pediatric acute lymphoblastic leukemia. Leukemia 2002;16:2222–2227.

    Article  PubMed  CAS  Google Scholar 

  20. Mrozek K, Heinonen K, Theil KS, et al. Spectral karyotyping in patients with acute myeloid leukemia and a complex karyotype shows hidden aberrations, including recurrent overrepresentation of 21q, 11q, and 22q. Genes Chromosomes Cancer. 2002;34:137–153.

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Hematology, General Hospital of the PLA, 100853, Beijing, China

    Bo Guo, Wanming Da & Xiaoping Han

Authors
  1. Bo Guo
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  2. Wanming Da
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  3. Xiaoping Han
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Corresponding author

Correspondence to Wanming Da.

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Guo, B., Da, W. & Han, X. The application of spectral karyotyping in leukemia. Chin. J. Clin. Oncol. 3, 254–257 (2006). https://doi.org/10.1007/s11805-006-0051-y

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  • DOI: https://doi.org/10.1007/s11805-006-0051-y

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