Abstract
Globally, various types of pollution affect coastal waters as a result of human activities. Bioaugmentation and biostimulation are effective methods for treating water pollution. However, few studies have explored the response of coastal prokaryotic and eukaryotic communities to bioaugmentation and biostimulation. Here, a 28-day outdoor mesocosm experiment with two treatments (bioaugmentation-A and combined treatment of bioaugmentation and biostimulation-AS) and a control (untreated-C) were carried out. The experiment was conducted in Meishan Bay to explore the composition, dynamics, and co-occurrence patterns of prokaryotic and eukaryotic communities in response to the A and AS using 16S rRNA and 18S rRNA gene amplicon sequencing. After treatment, Gammaproteobacteria and Epsilonproteobacteria were significantly increased in group AS compared to group C, while Flavobacteriia and Saprospirae were significantly reduced. Dinoflagellata was significantly reduced in AS compared to C, while Chrysophyta was significantly reduced in both AS and A. Compared to C, the principal response curve analyses of the prokaryotic and eukaryotic communities both showed an increasing trend followed by a decreasing trend for AS. Furthermore, the trends of prokaryotic and eukaryotic communities in group A were similar to those in group AS compared with group C, but AS changed them more than A did. According to the species weight table on principal response curves, a significant increase was observed in beneficial bacteria in prokaryotic communities, such as Rhodobacterales and Oceanospirillales, along with a decrease in autotrophs in eukaryotic communities, such as Chrysophyta and Diatom. Topological properties of network analysis reveal that A and AS complicate the interactions between the prokaryotic and eukaryotic communities. Overall, these findings expand our understanding of the response pattern of the bioaugmentation and biostimulation on coastal prokaryotic and eukaryotic communities.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (No. 42077219), the Ningbo Municipal Natural Science Foundation (No. 2019A610443), the Hangzhou Municipal Agriculture and Social Development Project (No. 2020ZDSJ0697), and the Fundamental Research Funds for the Provincial Universities of Zhejiang (No. SJLY2020011).
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Yuan, F., Zhao, Y., Dai, Y. et al. Effects of Biostimulation-Bioaugmentation on Coastal Microbial Community in an in situ Mesocosm System. J. Ocean Univ. China 23, 233–246 (2024). https://doi.org/10.1007/s11802-024-5692-z
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DOI: https://doi.org/10.1007/s11802-024-5692-z