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The Response of Bio-Component Production of Nannochloris oculata to the Combinations of Monochromatic Light

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Abstract

Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis. The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investigated. The results indicated that a high proportion of Red-light enhanced the pigments and carbohydrate production but reduced those of the biomass and lipid. Monochromatic Blue-light has advantageous effects on lipid production compared with Red- and White-light. The optimal light spectrum for the protein production was the combination of Red- and Blue-light at a ratio of 4:3 or 5:2. Among the seven fatty acids identified from N. oculata, the contents of C16:0, C18:0, and C18:3(n-3) in the lipid were inhibited by the increased proportion of Red-light while those of C18:2(n-9), C16:2(n-6), and C20:0 were inhibited by Blue-light. Monochromatic Red- and Blue-light and their combinations were proposed as a promising illuminating strategy for the large-scale cultivation aiming for various bio-components.

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References

  • Almutairi, A. W., 2020. Effects of nitrogen and phosphorus limitations on fatty acid methyl esters and fuel properties of Dunaliella salina. Environmental Science and Pollution Research, 27: 32296–32303.

    Article  Google Scholar 

  • An, M., Gao, L., Zhao, W., Chen, W., and Li, M., 2020. Effects of nitrogen forms and supply mode on lipid production of microalga Scenedesmus obliquus. Energies, 13: 697.

    Article  Google Scholar 

  • Bligh, E. G., and Dyer, W. J., 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37: 911–917.

    Article  Google Scholar 

  • Bounnit, T., Saadaoui, I., Rasheed, R., Schipper, K., Muraikhi, M., and Al-Jabri, H., 2020. Sustainable production of Nannochloris atomus biomass towards biodiesel production. Sustainability, 12: 2008.

    Article  Google Scholar 

  • Bradford, M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248–254.

    Article  Google Scholar 

  • Bredda, E. H., Silva, A. F. D., Da Silva, M. B., and Da Rós, P. C. M., 2020. Mixture design as a potential tool in modeling the effect of light wavelength on Dunaliella salina cultivation: An alternative solution to increase microalgae lipid productivity for biodiesel production. Preparative Biochemistry and Biotechnology, 50(4): 379–389.

    Article  Google Scholar 

  • Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A, and Smith, F., 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350–356.

    Article  Google Scholar 

  • Fu, W., Guðmundsson, Ó., Paglia, G., Herjólfsson, G., Andrésson, Ó. S., Palsson, B. Ø., et al., 2013. Enhancement of carotenoid biosynthesis in the green microalga Dunaliella salina with light-emitting diodes and adaptive laboratory evolution. Applied Microbiology and Biotechnology, 97: 2395–2403.

    Article  Google Scholar 

  • Garali, S., Sahraoui, I., de la Iglesia, P., Mohamed, C., Diogène, J., Ksouri, J., et al., 2016. Effects of nitrogen supply on Pseudo-nitzschia calliantha, and Pseudo-nitzschia cf. seriata: Field and laboratory experiments. Ecotoxicology, 25: 1211–1225.

    Article  Google Scholar 

  • Guillard, R. R. L., and Ryther, J. H., 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea Cleve. Canadian Journal of Microbiology, 8: 229–239.

    Article  Google Scholar 

  • Han, F., Pei, H., Hu, W., Zhang, S., Han, L., and Ma, G., 2016. The feasibility of ultrasonic stimulation on microalgae for efficient lipid accumulation at the end of the logarithmic phase. Algal Research, 16: 189–194.

    Article  Google Scholar 

  • Han, S. I., Kim, S., Lee, C., and Choi, Y. E., 2019. Blue-Red LED wavelength shifting strategy for enhancing beta-carotene production from halotolerant microalga, Dunaliella salina. Journal of Microbiology, 57(2): 101–106.

    Article  Google Scholar 

  • Jin, C., Yu, B., Qian, S., Liu, Q., and Zhou X., 2021. Impact of combined monochromatic light on the bio-component productivity of Dunaliella salina. Journal of Renewable and Sustainable Energy, 13: 021101.

    Article  Google Scholar 

  • Kandilian, R., Lee, E., and Pilon, L., 2013. Radiation and optical properties of Nannochloropsis oculata grown under different irradiances and spectra. Bioresource Technology, 137: 63–73.

    Article  Google Scholar 

  • Kong, Q., Zhu, L., and Shen, X., 2010. The toxicity of naphthalene to marine Chlorella vulgaris under different nutrient conditions. Journal of Hazardous Materials, 178: 282–286.

    Article  Google Scholar 

  • Lamers, P. P., Janssen, M., De Vos, R. C., Bino, R. J., and Wijffels, R. H., 2012. Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga. Journal of Biotechnology, 162: 21–27.

    Article  Google Scholar 

  • Li, Y., Li, L., Liu, J., and Qin, R., 2020. Light absorption and growth response of Dunaliella under different light qualities. Journal of Applied Phycology, 32(2): 1041–1052.

    Article  Google Scholar 

  • Lin, C. Y., and Tseng, Y. M., 2018. Effects of LED irradiation and sea water culture on the lipid characteristics of Nannochloropsis oculata. Journal of Renewable & Sustainable Energy, 10(2): 023102.

    Article  Google Scholar 

  • López-Rosales, A., Ancona-Canché, K., Chavarria-Hernandez, J., Barahona-Pérez, F., Toledano-Thompson, T., Garduño-Solorzano, G., et al., 2019. Fatty acids, hydrocarbons and terpenes of Nannochloropsis and Nannochloris isolates withpotential for biofuel production. Energies, 12(1): 130.

    Article  Google Scholar 

  • Park, S. J., Choi, Y. E., Kim, E. J., Park, W. K., Kim, C., and Yang, J. W., 2012. Serial optimization of biomass production using microalga Nannochloris oculata and corresponding lipid biosynthesis. Bioprocess & Biosystems Engineering, 35: 3–9.

    Article  Google Scholar 

  • Qari, H. A., and Oves, M., 2020. Fatty acid synthesis by Chlamydomonas reinhardtii in phosphorus limitation. Journal of Bioenergetics and Biomembranes, 52: 27–38.

    Article  Google Scholar 

  • Severes, A., Hegde, S., D’Souza, L., and Hegde, S., 2017. Use of light emitting diodes (LEDs) for enhanced lipid production in micro-algae based biofuels. Journal of Photochemistry & Photobiology B: Biology, 170: 235–240.

    Article  Google Scholar 

  • Subhash, V. G., Chugh, N., Iyer, S., Waghmare, A., Musale, A., Nandru, R., et al., 2020. Application of in vitro protein solubility for selection of microalgae biomass as protein ingredient in animal and aquafeed. Journal of Applied Phycology, 32: 3955–3970.

    Article  Google Scholar 

  • Sujitha, B. S., and Muthu, A., 2016. Inflfluence of abscisic acid on growth, biomass and lipid yield of Scenedesmus quadriccauda under nitrogen starved condition. Bioresource Technology, 213: 198–203.

    Article  Google Scholar 

  • Sukhija, P. S., and Palmquist, D. I., 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and faces. Journal of Agriculture and Food Chemistry, 36: 1202–1206.

    Article  Google Scholar 

  • Vadiveloo, A., Moheimani, N. R., and Cosgrove, J. J., 2015. Effect of different light spectra on the growth and productivity of acclimated Nannochloropsis sp. (Eustigmatophyceae). Algal Research, 8: 121–127.

    Article  Google Scholar 

  • Wang, S., Liu, J., Li, C., and Chung, B. M., 2019. Efficiency of Nannochloropsis oculata and Bacillus polymyxa symbiotic composite at ammonium and phosphate removal from synthetic wastewater. Environmental Technology, 40: 2494–2503.

    Article  Google Scholar 

  • Yoshifumi, U., Shimpei, A., Akihiko, K., and Seiji, A., 2019. Adaptation of light-harvesting functions of unicellular green algae to different light qualities. Photosynthesis Research, 139: 145–154.

    Article  Google Scholar 

  • Yuan, H., Zhang, X., Jiang, Z., Wang, X., Wang, Y., Cao, L., et al., 2020. Effect of light spectra on microalgal biofilm: Cell growth, photosynthetic property, and main organic composition. Renewable Energy, 157: 83–89.

    Article  Google Scholar 

  • Zhang, X., Yuan, H., Guan, L., Wang, X., and Zhang, X., 2019. Influence of photoperiods on microalgae biofilm: Photosynthetic performance, biomass yield, and cellular composition. Energies, 12: 3724.

    Article  Google Scholar 

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Nos. 41776156 and 42177459), the Open-End Funds of Jiangsu Key Laboratory of Marine Bioresources and Environment (No. SH20201206).

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Authors and Affiliations

  1. College of Environmental Science & Engineering, Yangzhou University, Yangzhou, 225127, China

    Binqi Yu, Shouyuan Qian, Qing Liu, Cuili Jin & Xiaojian Zhou

  2. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, 222005, China

    Cuili Jin

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  1. Binqi Yu
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  2. Shouyuan Qian
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  3. Qing Liu
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  4. Cuili Jin
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  5. Xiaojian Zhou
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Correspondence to Cuili Jin or Xiaojian Zhou.

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Yu, B., Qian, S., Liu, Q. et al. The Response of Bio-Component Production of Nannochloris oculata to the Combinations of Monochromatic Light. J. Ocean Univ. China 21, 243–251 (2022). https://doi.org/10.1007/s11802-022-4896-3

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  • DOI: https://doi.org/10.1007/s11802-022-4896-3

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