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Inhibition of Breast Cancer Resistance Protein (BCRP) by Ko143 Can Affect Pharmacokinetics of Enrofloxacin in Exopalaemon carinicauda

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Abstract

Adenosine triphosphate-binding cassette transporter breast cancer resistance protein (BCRP) exists highly in the apical membranes of epithelia, and is involved in drug availability. Ko143 is a typical inhibitor of BCRP in rodents. The synthetic antibacterial agent enrofloxacin (ENRO) is a fluoroquinolone employed as veterinary and aquatic medicine, and also a substrate for BCRP. BCRP gene highly expressed in the hepatopancreas and intestine of Exopalaemon carinicauda as was determined with real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) method. The effects of Ko143 on the abundance of BCRP mRNA and ENRO pharmacokinetics in E. carinicauda were studied. The mRNA abundance of BCRP decreased significantly in hepatopancreas and intestine (P <0.05) after Ko143 treatment. Co-administration of Ko143 significantly changed the pharmacokinetics of orally administered enrofloxacin, which was supported by higher distribution half-life (t1/2α), elimination half-life (t1/2β), area under the curve up to the last measurable concentration (AUC0-t), peak concentration (Cmax) and lower clearance (CL/F). These findings revealed that Ko143 downregulates BCRP expression in hepatopancreas and intestine, thus affects the pharmacokinetics of orally administered enrofloxacin in E. carinicauda. The drug-drug interaction can be caused by the change in BCRP activity if ENRO is used in combination with other drugs in shrimp.

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Acknowledgements

This work was supported by the Natural Science Foundation of Shandong Province, P. R. China (No. ZR2019 QC015), the National Key R&D Program of China (No. 2019YFD0900403), the Central Public-Interest Scientific Institution Basal Research Fund, CAFS (Nos. 2019ZD09 03 and 2020TD46), the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) (No. 2018SDKJ0502-2), the Earmarked Fund for Modern Agro-industry Technology Research System (No. CARS-48), and the National Natural Science Foundation of China (No. 31873039).

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Authors and Affiliations

  1. Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China

    Qianqian Zhai, Yang Xu, Cuiping Li, Yanyan Feng, Yanting Cui, Li Ma & Jian Li

  2. Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Qianqian Zhai, Yang Xu, Cuiping Li, Yanyan Feng, Yanting Cui, Li Ma & Jian Li

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  1. Qianqian Zhai
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  2. Yang Xu
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  4. Yanyan Feng
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  6. Li Ma
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  7. Jian Li
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Correspondence to Qianqian Zhai or Jian Li.

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Zhai, Q., Xu, Y., Li, C. et al. Inhibition of Breast Cancer Resistance Protein (BCRP) by Ko143 Can Affect Pharmacokinetics of Enrofloxacin in Exopalaemon carinicauda. J. Ocean Univ. China 19, 1116–1124 (2020). https://doi.org/10.1007/s11802-020-4312-9

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  • DOI: https://doi.org/10.1007/s11802-020-4312-9

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