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Ion Transport Signal Pathways Mediated by Neurotransmitter (Biogenic Amines) of Litopenaeus vannamei Under Low Salinity Challenge

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Abstract

The Pacific white shrimp, Litopenaeus vannamei, is widely farmed in China. Salinity is a major environmental factor that affects its growth and distribution. Crustacean hyperglycemic hormone is verified to participate in ion transport in response to the salinity challenge mediated by endocrine neurotransmitters (biogenic amines, BAs). In the present study, the contents of BAs and expressions of their receptors were detected in gills of Litopenaeus vannamei exposed to low salinity. The intracellular signaling molecules such as cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), 14-3-3 protein, FXYD2 protein and cAMP response element-binding protein (CREB) were detected. The effects of low salinity on the expressions of Na+/K+-ATPase, Na+/K+/2Cl−co-transporter and Cl−transporter and activity of Na+/K+-ATPase were also analyzed. The results showed that dopamine and epinephrine concentrations and their receptor expressions were significantly affected by low salinity. The changes of cAMP and PKA were obvious and the expressions of 14-3-3 and FXYD2 peaked at early stages. However, the expression of CREB was only significantly up-regulated on day 9. The activity and expression of Na+-K+-ATPase (α subunit) reached a peak on day 1. The expressions of Na+/K+/2Cl−co-transporter and Cl−transporter up-regulated obviously. It suggests that BAs can activate the cAMP-PKA pathway, which further acts on the 14-3-3 and FXYD2 proteins, and ultimately improve the activity of Na+/K+-ATPase. Furthermore, after BAs stimulate the cAMP-PKA pathway, PKA phosphorylates the transcription factor CREB and regulates the expressions of ion transport enzymes/ transporters. The results in this study are helpful for understanding the response mechanism of endocrine neurotransmitters on osmoregulation in crustaceans.

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Acknowledgements

This work was supported by State Oceanic Administration Specific Public Project of China (No. 201305005) and the National Natural Science Foundation of China (No. 31072193). We thank all the laboratory members for their technical advice and helpful discussions.

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  1. The Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Luqing Pan, Lingjun Si & Dongxu Hu

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  1. Luqing Pan
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Correspondence to Luqing Pan.

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Pan, L., Si, L. & Hu, D. Ion Transport Signal Pathways Mediated by Neurotransmitter (Biogenic Amines) of Litopenaeus vannamei Under Low Salinity Challenge. J. Ocean Univ. China 18, 210–218 (2019). https://doi.org/10.1007/s11802-019-3713-0

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