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α-Glucosidase Inhibitory Activities of Lutein and Zeaxanthin Purified from Green Alga Chlorella ellipsoidea

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Abstract

α-Glucosidase inhibitors are used therapeutically to treat type-2 diabetes mellitus. Through a bioassay-guided fractionation technique, three carotenoids, (all-E)-lutein, (all-E)-zeaxanthin and (9-Z)-zeaxanthin, were purified from the green alga Chlorella ellipsoidea, in which (all-E)-lutein and (9-Z)-zeaxanthin had potent α-glucosidase inhibitory activity. IC50 values of (all-E)-lutein and (9-Z)-zeaxanthin were 70 and 53.5 μmol L−1 against Saccharomyces cerevisiae α-glucosidase, respectively, with non-competitive inhibition. In addition, IC50 values of (9-Z)-zeaxanthin against Bacillus stearothermophilus and rat-intestinal α-glucosidase were 805.1 and 671.2 μmol L−1, respectively. The Ki values of (all-E)-lutein and (9-Z)-zeaxanthin against S. cerevisiae α-glucosidase were 78.1 and 16.5 μmol L−1, respectively. Therefore, C. ellipsoidea carotenoids might be utilized as a novel candidate to prevent type-2 diabetes mellitus related disorders in food and medical industry.

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Acknowledgements

This research was a part of the project titled ‘Future Marine Technology Development’ funded by the Ministry of Oceans and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Jia Qi

  2. Department of Marine Food Science and Technology, Gangneung-Wonju National University, Gangneung, 25457, Republic of Korea

    Jia Qi & Sang Moo Kim

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  1. Jia Qi
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  2. Sang Moo Kim
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Correspondence to Sang Moo Kim.

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Qi, J., Kim, S.M. α-Glucosidase Inhibitory Activities of Lutein and Zeaxanthin Purified from Green Alga Chlorella ellipsoidea. J. Ocean Univ. China 17, 983–989 (2018). https://doi.org/10.1007/s11802-018-3465-2

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  • DOI: https://doi.org/10.1007/s11802-018-3465-2

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