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Transcriptome Profiling of the Abdominal Skin of Larimichthys crocea in Light Stress

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Abstract

Large yellow croaker (Larimichthys crocea), one of the most important marine fish species in China, can change its abdominal skin color when it is shifted from light to dark or from dark to light, providing us an opportunity of investigating the molecular responding mechanism of teleost in light stress. The gene expression profile of fish under light stress is rarely documented. In this research, the transcriptome profiles of the abdominal skin of L. crocea exposed to light or dark for 0 h, 0.5 h and 2 h were produced by next-generation sequencing (NGS). The cluster results demonstrated that stress period, rather than light intensity (e.g., light or dark), is the major influencing factor. Differently expressed genes (DEGs) were identified between 0 h and 0.5 h groups, between 0 h and 2 h groups, between 0.5 h light and 0.5 h dark, and between 2 h light and 2 h dark, respectively. The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation revealed that the genes relating to immunity, energy metabolism, and cytoskeletal protein binding were significantly enriched. The detailed analysis of transcriptome profiles also revealed regular gene expression trends, indicating that the elaborate gene regulation networks underlined the molecular responses of the fish to light stress. This transcriptome analysis suggested that systematic and complicated regulatory cascades were functionally activated in response to external stress, and coloration change caused by light stress was mainly attributed to the change in the density of chromatophores for L. crocea. This study also provided valuable information for skin coloration or light stress research on other marine fish species.

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Acknowledgements

This work was supported by grants from the National Natural Science Foundation of China (No. U1205122), Key projects of the Xiamen Southern Ocean Research Center (14GZY70NF34), the Natural Science Foundation of Fujian Province (No. 2015J05069), ‘Li ShangDa’ Foundation and Innovation Research Team Foundation of Jimei University (No. 2010A02). The authors thank Wanbo Li and Edna Falcis Salcedo as they revised the manuscript and gave a lot of helpful suggestions.

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Authors and Affiliations

  1. Key Laboratory of Healthy Mariculture for the East China Sea of Ministry of Agriculture, Fisheries College, Jimei University, Xiamen, 361021, China

    Zhaofang Han, Changhuan Lv, Shijun Xiao, Kun Ye, Dongling Zhang & Zhiyong Wang

  2. Institute of Biomedical Sciences, Mackay Medical College, New Taipei City, Taiwan, China

    Huai Jen Tsai

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  1. Zhaofang Han
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  2. Changhuan Lv
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Correspondence to Zhiyong Wang.

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Han, Z., Lv, C., Xiao, S. et al. Transcriptome Profiling of the Abdominal Skin of Larimichthys crocea in Light Stress. J. Ocean Univ. China 17, 344–354 (2018). https://doi.org/10.1007/s11802-018-3372-6

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  • DOI: https://doi.org/10.1007/s11802-018-3372-6

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