Journal of Ocean University of China

, Volume 16, Issue 6, pp 1003–1008 | Cite as

Chlorophyll fluorescence extraction from water-leaving radiance of algae-containing water through polarization

  • Lin Wang
  • Zhongfeng Qiu
  • Huifang Pang
  • Yongjian Liu
  • Yanlong Chen
  • Lingling Jiang
Article
  • 26 Downloads

Abstract

When measuring reflectance spectra, it is very important to accurately extract chlorophyll fluorescence from elastic- scattering light in water-leaving radiance. The elastic scattering of light by water particles produces partially polarized light. In contrast, chlorophyll fluorescence in planktonic algae yields completely unpolarized light. These properties can be used to separate fluorescent signals from the water-leaving radiance and thus to determine chlorophyll concentration. The algal species Aureococcus anophagefferens was used to conduct a laboratory polarization experiment. For the tests, we used a field spectroradiometer and a polarizer; measurements were collected using two different observation modes. The chlorophyll fluorescence curve extracted through polarization shows an excellent match with the results obtained using the fluorospectro photometer for both measurement modes, suggesting that polarization-based chlorophyll fluorescence extraction may be feasible. The extracted fluorescence is more reliable at incident zenith angles ranging from 30° to 60°. For algae-containing water, the results improve with increasing chlorophyll concentration. This method could help improve chlorophyll concentration measurement and the remote-sensing detection of resulting harmful algae blooms.

Key words

chlorophyll fluorescence polarization technique water-leaving radiance extraction red shift 

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Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 41406199, 41506197), the Program Foundation of Nanjing University of Information Science and Technology (No. KHYS1301), the Doctoral Scientific Research Foundation of Liaoning Province (No. 201501190), and the Fundamental Research Funds for the Central Universities (No. 3132015081).

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Copyright information

© Science Press, Ocean University of China and Springer-Verlag GmbH Germany, part of Springer Nature 2017

Authors and Affiliations

  • Lin Wang
    • 1
  • Zhongfeng Qiu
    • 2
  • Huifang Pang
    • 1
    • 3
  • Yongjian Liu
    • 1
  • Yanlong Chen
    • 1
  • Lingling Jiang
    • 3
  1. 1.Department of Ocean Remote SensingNational Marine Environmental Monitoring CenterDalianP. R. China
  2. 2.School of Marine SciencesNanjing University of Information Science and TechnologyNanjingP. R. China
  3. 3.College of Environmental Science and EngineeringDalian Maritime UniversityDalianP. R. China

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