Skip to main content
SpringerLink
Log in

Screening white spot syndrome virus (WSSV)-resistant molecular markers from Fenneropenaeus chinensis

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

White spot syndrome virus (WSSV)-resistant molecular markers were screened from the selectively bred new variety ‘Huanghai No. 2’ of Fenneropenaeus chinensis using unlabeled-probe high-resolution melting (HRM) technique. After the artificial infection with WSSV, the first 96 dead shrimps and the last 96 surviving shrimps were collected, representing WSSV-susceptible and -resistant populations, respectively. The genotypes at well-developed 39 single nucleotide polymorphisms (SNPs) loci were obtained. As revealed in the Chi-square test, 3 SNPs, genotype A/A of contig C364-89AT, genotype A/A of C2635-527CA and genotype C/T of contig C12355-592CT, were positively correlated with disease-resistance traits. Other 2 SNPs, genotype G/G of contig C283-145AG and genotype C/C of contig C12355-592CT, were negatively correlated. Moreover, analysis with BlastX program for disease-resistant SNPs indicated that 3 contigs, Contig283, Contig364 and Contig12355, matched to the functional genes of effector caspase of Penaeus monodon, peptide transporter family 1-like protein, and 40S ribosomal protein S2 of Perca flavescens with high sequence similarity. The results will be helpful to provide theoretical and technical supports for molecular marker-assisted selective breeding of F. chinensis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arts, J. A., Cornelissen, F. H., Cijsouw, T., Hermsen, T., Savelkoul, H. F., and Stet, R. J., 2007. Molecular cloning and expression of a Toll receptor in the giant tiger shrimp, Penaeus monodon. Fish Shellfish Immunolgy, 23 (3): 504–513.

    Article  Google Scholar 

  • Astrofsky, K. M., Roux, M. M., Klimple, K. R., Fox, J. G., and Dhar, A. K., 2002. Isolation of differentially expressed genes from white spot syndrome virus (WSV) infected Pacific blue shrimp (Penaeus stylirostris). Archives of Virology, 147: 1799–1812.

    Article  Google Scholar 

  • Chang, C. C., Yeh, M. S., Lin, H. K., and Cheng, W., 2008. The effect of vibrio alginolyticus infection on caspase-3 expression and activity in white shrimp Litopenaeus vannamei. Fish and Shellfish Immunology, 25 (5): 672–678.

    Article  Google Scholar 

  • Chou, H. Y., Huang, C. Y., Wang, C. H., Chiang, H. C., and Lo, C. F., 1995. Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Diseases of Aquatic Organisms, 23: 165–173.

    Article  Google Scholar 

  • Dhar, A. K., Dettroi, A., Roux, M. M., Klimpel, K. R., and Read, B., 2003. Identification of differential expressed genes in shrimp (Penaeus stylirostris) infected with white spot syndrome virus by cDNA microarrays. Archives of Virology, 148: 2381–2396.

    Article  Google Scholar 

  • Dong, S. R., Kong, J., Meng, X. H., Zhang, Q. W., Zhang, T. S., and Wang, R. C., 2008. Microsatellite DNA markers associated with resistance to WSSV in Penaeus (Fenneropenaeus) Chinensis. Aquaculture, 282 (1-4): 138–141.

    Article  Google Scholar 

  • Durand, S. V., and Lighter, D. V., 2002. Quantitative real-time PCR for the measurement of white spot syndrome virus in shrimp. Journal of Fish Diseases, 25: 381–389.

    Article  Google Scholar 

  • Ganapathy, V., Brandsch, M., and Leibach, F. H., 1994. Physiology of the Gastro-Intestinal Tract. Johnson, L. R., ed., 3rd edition, Raven Press, New York, 1773–1794.

  • Gao, H., Lai, X. F., Meng, X. H., Luo, K., and Kong, J., 2011. Expression of three kinds of peneaidins after WSSV challenge and their SNPs detection in Chinese shrimp, Fenneropenaeus chinensis. Journalof Fishery Sciences of China, 18 (3): 646–653.

    Article  Google Scholar 

  • He, J. G., Zhou, H. M., Yao, B., Yang, X. M., and Deng, M., 1999. White spot syndrome baculovirus (WSBV) host range and transmission route. Acta Scientiarum Naturalium Universitatis Sunyatseni, 38 (2): 65–69.

    Google Scholar 

  • He, N., Qin, Q., and Xu, X., 2005. Differential profile of genes expressed in hemocytes of white spot syndrome virus-resistant shrimp (Penaeus japonicus) by combining suppression subtractive hybridization and differential hybridization. Antiviral Research, 66: 39–45.

    Article  Google Scholar 

  • Kong, J., Luo, K., Luan, S., Wang, Y. Y., Zhang, Q. W., Zhang, T. S., Meng, X. H., Wang, W. J., and Ruan, X. H., 2012. The new variety of Fenneropenaeus chinensis ‘Huanghai No.2’. Journal of Fisheries of China, 36 (12): 1854–1862.

    Article  Google Scholar 

  • Lai, X. F., Kong, J., Wang, Q. Y., and Wang, W. J., 2010. Analysis of molecular basis related to WSSF-resistance in Fenneropenaeus chinensis by mRNA differential Display PCR. Journal of Fishery Sciences of China, 17 (2): 193–200.

    Google Scholar 

  • Li, L., Song, X., and Wang, D. H., 2008. Peptide transporters and drug transport. The Chinese Journal of Clinical Pharmacology, 17 (1): 61–64.

    Google Scholar 

  • Li, S. H., Zhang, T. S., Kong, J., and Meng, X. H., 2009. Screen of white-spot-syndrome-virus (WSSV)-resistance molecular markers and analysis of genetic diversity in Fenneropenaeus chinensis. Progress in Fishery Sciences, 30 (2): 54–59.

    Google Scholar 

  • Li, Z. X., Li, J., Wang, Q. Y., He, Y. Y., and Liu, P., 2006. AFLP-based genetic linkage map of marine shrimp (Penaeus Fenneropenaeus) chinensis. Aquaculture, 261: 463–472.

    Article  Google Scholar 

  • Liu, P., Kong, J., Meng, X. H., Liu, Z. H., and Li, J., 2000. Transmission route of white spot syndrome virus (WSSV) in shrimp farming process. Marine Fisheries Research, 21 (3): 9–12.

    Google Scholar 

  • Liu, P., Meng, X. H., Kong, J., Li, J., and Wang, W. J., 2002. RAPD analysis of genetic markers affiliated with disease resistant trait in shrimp. Journal of Fisheries of China, 26 (3): 270–274.

    Google Scholar 

  • Liu, T. H., and Chen, R., 2003. Caspases and ischemic cerebrovascular diseases. Joural of Capital Medical University, 24 (2): 208–211.

    Google Scholar 

  • Luo, T., Zhang, X., Shao, Z., and Xu, X., 2003. PmAV, a novel gene involved in virus resistance of shrimp Penaeus monodon. FEBS Letters, 551: 53–57.

    Article  Google Scholar 

  • Mark, T., 2007. Bedford arginine methylation at a glance. Journal of Cell Science, 120 (24): 4243–4246.

    Article  Google Scholar 

  • Meng, X. H., Kong, J., Liu, P., Ma, C. Y., and Li, Y., 2005. Screen of white spot syndrome virus (WSSV) resistance molecular markers in Fenneropenaeus chinensis. Journal of Fishery Sciences of China, 12 (1): 14–19.

    Google Scholar 

  • Meng, X. H., Liu, P., Kong, J., Li, J., and Wang, W. J., 2002. RAPD analysis of genetic markers affiliated with disease resistant trait in shrimp. Journal of Fishery Sciences of China, 26 (3): 270–274.

    Google Scholar 

  • Pan, D., He, N., Yang, Z., Liu, H., and Xu, X., 2005. Differential gene expression profile in hepatopancreas of WSSV-resistant shrimp (Penaeus japonicus) by suppression subtractive hybridization. Development & Comparative Immunology, 29: 103–112.

    Article  Google Scholar 

  • Paulsen, I. T., and Skurray, R. A., 1994. The POT family of transport proteins. Trends in Biochemical Sciences, 19 (10): 404.

    Article  Google Scholar 

  • Phongdara, A., Wanna, W., and Chotigeat, W., 2006. Molecular cloning and expression of caspase from white shrimp Penaeus merguiensis. Aquaculture, 252 (2-4): 114–120.

    Article  Google Scholar 

  • Rojtinnakorn, J., Hirono, I., Itami, T., Takahashi, Y., and Aoki, T., 2002. Gene expression in heamocytes of kuruma prawn, Penaeus japonicus, in response to infection with WSSV by EST approach. Fish and Shellfish Immunology, 13: 69–83.

    Article  Google Scholar 

  • Sambrook, J., Fritsch, E. F., and Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York, 253pp.

    Google Scholar 

  • Song, G. N., Jin, S. J., Zhang, J. Q., and Xiang, J. H., 2010. Cloning and expression of a caspase gene in Chinese shrimp Fenneropenaeus chinensis. Marine Sciences, 34 (12): 1–7.

    Google Scholar 

  • Tonganunt, M., Nupan, B., Saengsakd, M., Suklour, S., Wanna, W., Senapin, S., Chotigeat, W., and Phongdara, A., 2008. The role of Pm-fortilin in protecting shrimp from white spot syndrome virus (WSSV) infection. Fish and Shellfish Immunology, 25 (5): 633–637.

    Article  Google Scholar 

  • Walker, P. J., Gudkovs, N., and Padiyar, P. A., 2011. Longitudinal disease studies in small-holder black tiger shrimp (Penaeus monodon) farms in Andhra Pradesh, India. I. High prevalence of WSSV infection and low incidence of disease outbreaks in BMP ponds. Aquaculture, 318 (3-4): 277–282.

    Google Scholar 

  • Wang, B., Li, F., Dong, B., Zhang, X., Zhang, C., and Xiang, J., 2006. Discovery of the genes in response to white spot syndrome virus (WSSV) infection in Fenneropenaeus chinensis through cDNA microarray. Marine Biotechnology (NY), 8: 491–500.

    Article  Google Scholar 

  • Wang, L., Zhi, B., Wu, W., and Zhang, X., 2008. Requirement for shrimp caspase in apoptosis against virus infection. Development & Comparative Immunology, 6: 706–715.

    Article  Google Scholar 

  • Wang, W. J., Zhang, T. S., Yang, C. H., Luan, S., Kong, J., and Wang, Q. Y., 2008. QTL associated with WSSV-resistance and growth-related traits in the Chinese shrimp Fenneropenaeus chinensis. Acta Zoologica Sinica, 54 (6): 1075–1081.

    Google Scholar 

  • Wongprasert, K., Sangsuriya, P., Phongdara, A., and Senapin, S., 2007. Cloning and characterization of a caspase gene from black tiger shrimp (Penaeus monodon) infected with white spot syndrome virus (WSSV). Journal of Biotechnology, 131 (1): 9–19.

    Article  Google Scholar 

  • Wu, W. L., and Zhang, X., 2007. Characterization of a Rab GTPase up-regulated in the shrimp Peneaus japonicus by virus infection. Fish and Shellfish Immunology, 23 (2): 438–445.

    Article  Google Scholar 

  • Wu, Y. Y., Meng, X. H., Kong, J., Wang, Q. Y., Luan, S., and Luo, K., 2013. Application of unlabeled probe by HRM in development of EST-SNPs in Fenneropenaeus chinesis. Progress in Fishery Sciences, 34 (1): 111–118.

    Google Scholar 

  • Yang, C. H., Kong, J., Wang, Q. Y., Tian, Y., Luo, K., and Zhang, T. S., 2007. Effects of inbreeding on growth and WSSV resistance of the juvenile Chinese shrimp, Fenneropenaeus chinensis. Journal of Fishery Sciences of China, 31 (2): 226–234.

    Google Scholar 

  • Yue, Z. Q., Wang, W. J., Kong, J., and Dai, J. X., 2005. AFLP analysis of four selected generations on disease-resistance trait of Fenneropenaeus chinensis. Journal of Fishery Sciences of China, 29 (1): 13–19.

    Google Scholar 

  • Zhang, M., Wang, H., Li, D., and Xu, X., 2009. A novel focal adhesion kinase from Marsupenaeus japonicus and its response to WSSV infection. Development & Comparative Immunology, 33 (4): 533–539.

    Article  Google Scholar 

Download references

Acknowledgments

This study was supported by the Taishan Scholar Program for Seed Industry, the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02) and National Natural Science Foundation of China (No. 31372523).

Author information

Authors and Affiliations

  1. Marine Biology Institute of Shandong Province, Qingdao, 266002, P. R. China

    Yingying Wu & Yongyun Zheng

  2. Laboratory for Marine Fisheries Science and Food Production Processes of Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, P. R. China

    Xianhong Meng, Jie Kong, Sheng Luan, Kun Luo & Qingyin Wang

  3. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China

    Xianhong Meng, Jie Kong, Sheng Luan & Kun Luo

Authors
  1. Yingying Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xianhong Meng
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jie Kong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sheng Luan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kun Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Qingyin Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yongyun Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Qingyin Wang.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wu, Y., Meng, X., Kong, J. et al. Screening white spot syndrome virus (WSSV)-resistant molecular markers from Fenneropenaeus chinensis . J. Ocean Univ. China 16, 129–136 (2017). https://doi.org/10.1007/s11802-017-3136-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-017-3136-8

Key words

Search

Navigation