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Seasonal variation of biochemical components in clam (Saxidomus purpuratus Sowerby 1852) in relation to its reproductive cycle and the environmental condition of Sanggou Bay, China

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Abstract

Seasonal variation of biochemical components in clam (Saxidomus purpuratus Sowerby 1852) was investigated from March 2012 to February 2013 in relation to environmental condition of Sanggou Bay and the reproductive cycle of clam. According to the histological analysis, the reproductive cycle of S. purpuratus includes two distinctive phases: a total spent and inactive stage from November to January, and a gametogenesis stage, including ripeness and spawning, during the rest of the year. Gametes were generated at a low temperature (2.1°C) in February. Spawning took place once a year from June to October. The massive spawning occurred in August when the highest water temperature and chlorophyll a level could be observed. The key biochemical components (glycogen, protein and lipid) in five tissues (gonad, foot, mantle, siphon and adductor muscle) were analyzed. The glycogen content was high before gametogenesis, and decreased significantly during the gonad development in the gonad, mantle and foot of both females and males, suggesting that glycogen was an important energy source for gonad development. The protein and lipid contents increased in the ovary during the gonad development, demonstrating that they are the major organic components of oocytes. The lipid and protein contents decreased in the testis, implying that they can provide energy and material for spermatogenesis. The results also showed that protein stored in the mantle and foot could support the reproduction after the glycogen was depleted.

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References

  • Ansari, Z., Parulekar, A. H., and Matondkar, S. G. P., 1981. Seasonal changes in meat weight and biochemical composition in the black clam Villorita cyprinoides (Grey). Indian Journal of Marine Sciences, 10(2): 128–131.

    Google Scholar 

  • Arellano-Martínez, M., Racotta, I. S., Ceballos-Vázquez, B. P., and Elorduy-Garay, J. F., 2004. Biochemical composition, reproductive activity and food availability of the lion’s paw scallop Nodipecten subnodosus in the Laguna Ojo de Liebre, Baja California Sur, Mexico. Journal of Shellfish Research, 23(1): 15–24.

    Google Scholar 

  • Barber, B. J., and Blake, N. J., 1981. Energy storage and utilization in relation to gametogenesis in Argopecten irradians concentricus (Say). Journal of Experimental Marine Biology and Ecology, 52(2): 121–134.

    Article  Google Scholar 

  • Barber, B. J., and Blake, N. J., 1985. Intra-organ biochemical transformations associated with oogenesis in the bay scallop, Argopecten irradians concentricus (Say), as indicated by 14C incorporation. Biological Bulletin, 168(1): 39–49.

    Article  Google Scholar 

  • Bayne, B. L., 1976. Aspects of reproduction in bivalve molluscs. In: Estuarine Processes. Wiley, M. L., ed., Academic Press, New York, 432–448.

    Google Scholar 

  • Bayne, B. L., Bubel, A., Gabbott, P. A., Livingstone, D. R., Lowe, D. M., and Moore, M. N., 1982. Glycogen utilization and gametogenesis in Mytilus edulis L. Marine Biology Letters, 3(2): 89–105.

    Google Scholar 

  • Beltran-Lugo, A. I., Maeda-Martinez, A. N., Pacheco-Aguilar, R., and Nolasco-Soria, H. G., 2006. Seasonal variations in chemical, physical, textural, and microstructural properties of adductor muscles of Pacific lions-paw scallop (Nodipecten subnodosus). Aquaculture, 258: 619–632.

    Article  Google Scholar 

  • Beninger, P. G., and Lucas, A., 1984. Seasonal variations in condition, reproductive activity, and gross biochemical composition of two species of adult clam reared in a common habitat: Tapes decussatus L. (Jeffreys) and Tapes philippinarum (Adams & Reeve). Journal of Experimental Marine Biology and Ecology, 79(1): 19–37.

    Article  Google Scholar 

  • Berthelin, C., Kellner, K., and Mathieu, M., 2000. Storage metabolism in the Pacific oyster (Crassostrea gigas) in relation to summer mortalities and reproductive cycle (West Coast of France). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 125(3): 359–369.

    Article  Google Scholar 

  • Bradford, M. M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72 (1-2): 248–254.

    Article  Google Scholar 

  • Carrasco, C. A., Navarro, J. M., and Leiva, G. E., 2006. Biochemical composition and tissue weight of Chorus giganteus (Gastropoda: Muricidae) exposed to different diets and temperatures during reproductive conditioning. Interciencia, 31(5): 376–381.

    Google Scholar 

  • Chavez-Villalba, J., Pommier, J., Andriamiseza, J., Pouvreau, S., Barret, J., Cochard, J. C., and Le Pennec, M., 2002. Broodstock conditioning of the oyster Crassostrea gigas: Origin and temperature effect. Aquaculture, 214: 115–130.

    Article  Google Scholar 

  • China State Bureau of Technical Supervision (CSBTS), 2007. The specification for marine monitoring: Ecological survey for offshore pollution and biological monitoring (GB 17378.7-2007), 24–25.

    Google Scholar 

  • Cho, E. S., Seo, Y. I., and Suh, Y. S., 2013. Genetic analysis of the purplish Washington clam (Saxidomus purpuratus Sowerby) of Korean coastal waters. Journal of Environmental Biology, 34(3): 613–621.

    Google Scholar 

  • Choi, J. W., Kim, S. K., Choi, Y. S., Lee, C. H., Lee, W. J., and Ryu, T. K., 2003. Optimal conditions for artificial fertilization, embryonic development, and larval growth of the purple clam, Saxidomus Purpuratus from southern coast of Korea. The Korean Journal of Malacology, 19(1): 33–40.

    Google Scholar 

  • Chung, E. Y., Kim, S. Y., Park, K. H., and Park, G. M., 2002. Sexual maturation, spawning, and deposition of the egg capsules of the female purple shell, Rapana venosa (Gastropoda: Muricidae). Malacologia, 44(2): 241–257.

    Google Scholar 

  • Delgado, M., Pérez Camacho, A., Labarta, U., and Fernández-Reiriz, M. J., 2004. The role of lipids in the gonadal development of the clam Ruditapes decussatus (L.). Aquaculture, 241: 395–411.

    Article  Google Scholar 

  • Dridi, S., Romdhane, M. S., and Elcafsi, M., 2007. Seasonal variation in weight and biochemical composition of the Pacific oyster, Crassostrea gigas in relation to the gametogenic cycle and environmental conditions of the Bizert lagoon, Tunisia. Aquaculture, 263: 238–248.

    Article  Google Scholar 

  • Gabbott, P. A., 1983. Developmental and seasonal metabolic activities in marine molluscs. In: The Mollusca, Vol. 2. Hochachka, P. W., ed., Academic Press, New York, 165–217.

    Google Scholar 

  • Gharsallah, I. H., Vasconcelos, P., Zamouri-Langar, N., and Missaoui, H., 2010. Reproductive cycle and biochemical composition of Hexaplex trunculus (Gastropoda: Muricidae) from Bizerte lagoon, northern Tunisia. Aquatic Biology, 10(2): 155–166.

    Article  Google Scholar 

  • Giese, A. C., 1969. A new approach to the biochemical composition of the mollusc body. Oceanography and Marine Biology: An Annual Review, 7: 175–229.

    Google Scholar 

  • Hahn, K. O., 1994. Gametogenic cycle of the Japanese abalone (ezoawabi), Haliotis-discus hannai, during conditioning with effective accumulative temperature. Aquaculture, 122: 227–236.

    Article  Google Scholar 

  • Helm, M. M., Holland, D. L., and Stephenson, R. R., 1973. The effect of supplementary algal feeding of a hatchery breeding stock of Ostrea edulis L. on larval vigour. Journal of the Marine Biological Association of the United Kingdom, 53(3): 673–684.

    Article  Google Scholar 

  • Holland, D. L., 1978. Lipid reserves and energy metabolism in the larvae of benthic marine invertebrates. In: Biochemical and Biophysical Perspectives in Marine Biology. Malin, D. C., and Sargent, J. R., eds., Academic Press, London, 85–123.

    Google Scholar 

  • Horikoshi, H., 1958. Glycogen. Chemical Field, 34: 36–39 (in Japanese).

    Google Scholar 

  • Ke, Q. Z., and Li, Q., 2013. Annual dynamics of glycogen, lipids, and proteins during the reproductive cycle of the surf clam Mactra veneriformis from the north coast of Shandong Peninsular, China. Invertebrate Reproduction & Development, 57(1): 49–60.

    Article  Google Scholar 

  • Kim, S. K., Rosenthal, H., Clemmesen, C., Park, K., Kim, D., Choi, Y., and Seo, H., 2005. Various methods to determine the gonadal development and spawning season of the purplish Washington clam, Saxidomus purpuratus (Sowerby). Journal of Applied Ichthyology, 21(2): 101–106.

    Article  Google Scholar 

  • Kim, Y. H., Ryu, D. K., Lee, D. W., Chang, D. S., Jong, B. K., Kim, S. T., and Kwon, D. H., 2006. Morphological analysis among populations of purpulish Washington clam, Saxidomus purpuratus on the Korean waters. The Korean Journal of Malacology, 22(1): 23–26.

    Google Scholar 

  • Lambert, P., and Dehnel, P. A., 1974. Seasonal variations in biochemical composition during the reproductive cycle of the intertidal gastropod Thais lamellosa Gmelin (Gastropoda, Prosobranchia). Canadian Journal of Zoology, 52(3): 305–318.

    Article  Google Scholar 

  • Laruelle, F., Guillou, J., and Paulet, Y. M., 1994. Reproductive pattern of the clams, Ruditapes decussatus and R. philippinarum on intertidal flats in Brittany. Journal of the Marine Biological Association of the United Kingdom, 74(2): 351–366.

    Article  Google Scholar 

  • Lee, C. H., Han, G. M., and Choi, J. W., 2005. Substrate selection for larval settlement and spat growth in the purple clam, Saxidomus purpuratus (Sowerby) in laboratory culture. The Korean Journal of Malacology, 21(1): 65–70.

    Google Scholar 

  • Lee, J. Y., Yang, H. S., Kang, D. H., Jeung, H. D., Hong, H. K., Lee, H. J., Kang, H. S., and Choi, K. S., 2014. Spatial variation in the annual reproductive cycle of Turbo (Batillus) cornutus (Gastropoda: Trochidae) at Jeju Island, Korea. Invertebrate Reproduction & Development, 58(1): 23–33.

    Article  Google Scholar 

  • Li, Q., Liu, W. G., Shirasu, K., Chen, W. M., and Jiang, S. X., 2006. Reproductive cycle and biochemical composition of the Zhe oyster Crassostrea plicatula Gmelin in an eastern coastal bay of China. Aquaculture, 261(2): 752–759.

    Article  Google Scholar 

  • Li, Q., Yang, L., Ke, Q. Z., and Kong, L. F., 2011. Gametogenic cycle and biochemical composition of the clam Mactra chinensis (Mollusca: Bivalvia): Implications for aquaculture and wild stock management. Marine Biology Research, 7(4): 407–415.

    Article  Google Scholar 

  • Li, Y., Qin, J. G., Li, X., and Benkendorff, K., 2009. Monthly variation of condition index, energy reserves and antibacterial activity in Pacific oysters, Crassostrea gigas, in Stansbury (South Australia). Aquaculture, 286: 64–71.

    Article  Google Scholar 

  • Liu, W. G., Li, Q., and Kong, L. F., 2013. Reproductive cycle and seasonal variations in lipid content and fatty acid composition in gonad of the cockle Fulvia mutica in relation to temperature and food. Journal of Ocean University of China, 12(3): 427–433.

    Article  Google Scholar 

  • Mathieu, M., and Lubet, P., 1993. Storage tissue metabolism and reproduction in marine bivalves–A brief review. Invertebrate Reproduction & Development, 23 (2-3): 123–129.

    Article  Google Scholar 

  • Napolitano, G. E., MacDonald, B. A., Thompson, R. J., and Ackman, R. G., 1992. Lipid composition of eggs and adductor muscle in giant scallops (Placopecten magellanicus) from different habitats. Marine Biology, 113(1): 71–76.

    Article  Google Scholar 

  • Ojea, J., Pazos, A. J., Martinez, D., Novoa, S., Sánchez, J. L., and Abad, M., 2004. Seasonal variation in weight and biochemical composition of the tissues of Ruditapes decussatus in relation to the gametogenic cycle. Aquaculture, 238: 451–468.

    Article  Google Scholar 

  • Park, H. J., Lee, W. C., Choy, E. J., Choi, K. S., and Kang, C. K., 2011. Reproductive cycle and gross biochemical composition of the ark shell Scapharca subcrenata (Lischke, 1869. reared on subtidal mudflats in a temperate bay of Korea. Aquaculture, 322: 149–157.

    Google Scholar 

  • Park, M. S., Kang, C. K., and Lee, P. Y., 2001. Reproductive cycle and biochemical composition of the ark shell Scapharca broughtonii (Schrenck) in a southern coastal bay of Korea. Journal of Shellfish Research, 20(1): 177–184.

    Google Scholar 

  • Peterson, C. H., 2002. Recruitment overfishing in a bivalve mollusc fishery: Hard clams (Mercenaria mercenaria) in North Carolina. Canadian Journal of Fisheries and Aquatic Sciences, 59(1): 96–104.

    Article  Google Scholar 

  • Ruan, F. T., Gao, S., Li, L., Qiu, Z. X., Zheng, Y. Y., and Li, Q., 2014. Gonad development and biochemical composition in the ark shell Scapharca broughtonii from coast of Shandong Peninsula. Journal of Fisheries of China, 38(1): 47–55 (in Chinese with English abstract).

    Google Scholar 

  • Ruiz, C., Martinez, D., Mosquera, G., Abad, M., and Sánchez, J., 1992. Seasonal variations in condition, reproductive activity and biochemical composition of the flat oyster, Ostrea edulis, from San Cibran (Galicia, Spain). Marine Biology, 112(1): 67–74.

    Article  Google Scholar 

  • Selin, N. I., 2014. The distribution, size, and age composition, and growth of Saxidomus purpurata (Sowerby, 1852. (Bivalvia: Veneridae) in Vostok Bay, the sea of Japan. Russian Journal of Marine Biology, 40(5): 375–382.

    Article  Google Scholar 

  • Serdar, S., and Lök, A., 2009. Gametogenic cycle and biochemical composition of the transplanted carpet shell clam Tapes decussatus, Linnaeus 1758 in Sufa (Homa) Lagoon, Izmir, Turkey. Aquaculture, 293: 81–88.

    Article  Google Scholar 

  • Soudant, P., Marty, Y., Moal, J., Robert, R., Quere, C., LeCoz, J. R., and Samain, J. F., 1996. Effect of food fatty acid and sterol quality on Pecten maximus gonad composition and reproduction process. Aquaculture, 143: 361–378.

    Article  Google Scholar 

  • Wei, L. P., Shu, Y. F., Guan, F., Han, Y. P., and Yu, Z. R., 1982. A preliminary survey on the biology of Saxidomus purpuratus. Journal of Fisheries of China, 6(1): 1–8 (in Chinese with English abstract).

    Google Scholar 

  • Wei, L. P., Wang, B. G., Sun, Y. T., Yang, F. S., and Wang, S. F., 1994. Study on resource enhancement technology of Saxidomus purpuratus. Fisheries Science & Technology Information, 21(1): 13–18 (in Chinese with English abstract).

    Google Scholar 

  • Yan, H. W., Li, Q., Liu, W. G., Yu, R. H., and Kong, L. F., 2010a. Seasonal changes in reproductive activity and biochemical composition of the razor clam Sinonovacula constricta (Lamarck 1818). Marine Biology Research, 6(1): 78–88.

    Article  Google Scholar 

  • Yan, H. W., Li, Q., Yu, R. H., and Kong, L. F., 2010b. Seasonal variations in biochemical composition and reproductive activity of venus clam Cyclina sinensis (Gmelin) from the Yellow River Delta in northern China in relation to environmental factors. Journal of Shellfish Research, 29(1): 91–99.

    Article  Google Scholar 

  • Yoon, J. M., and Park, S. Y., 2006. Genetic differences and variation in two purple Washington clam (Saxidomus purpuratus) populations from south and north Korea. The Korean Journal of Malacology, 22(2): 97–108.

    Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao, 266003, P. R. China

    Jinhong Bi & Qi Li

  2. Rongcheng Fishery Technical Extension Station, Rongcheng, 264300, P. R. China

    Jinhong Bi, Xinjun Zhang, Zhixin Zhang, Jinling Tian & Yushan Xu

  3. Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, P. R. China

    Wenguang Liu

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  1. Jinhong Bi
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Correspondence to Qi Li.

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Bi, J., Li, Q., Zhang, X. et al. Seasonal variation of biochemical components in clam (Saxidomus purpuratus Sowerby 1852) in relation to its reproductive cycle and the environmental condition of Sanggou Bay, China. J. Ocean Univ. China 15, 341–350 (2016). https://doi.org/10.1007/s11802-016-2855-6

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  • DOI: https://doi.org/10.1007/s11802-016-2855-6

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