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Characterization and expression analysis of B Cell receptor accessory molecule CD79 gene in humphead snapper (Lutjanus sanguineus)

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Abstract

CD79, a key component of the B cell antigen receptor complex, is composed of CD79α(Igα) and CD79β(Igβ) encoded by mb-1 and B29 respectively, and plays an important role in B cell signaling. In this study, we isolated and characterized mb-1 and B29 from humphead snapper (Lutjanus sanguineus). Their tissue distribution and expression profiles after stimulations in vitro and in vivo were also investigated. The humphead snapper mb-1 and B29 contain open reading frames of 684 bp and 606 bp, encoding 227 amino acids and 201 amino acids, respectively. Both CD79α and CD79β possess signal peptide, extracellular Ig domain, transmembrane region and immunoreceptor tyrosine kinase activation motif (ITAM). Mb-1 is highly expressed in lymphoid organs (thymus, posterior kidney and spleen) and mucosal-associated lymphoid tissues (gill and intestine), while B29 is mainly detected in posterior kidney, spleen, gill and skin. Furthermore, transcription of mb-1 and B29 in head kidney leucocytes was up-regulated following lipopolysaccharide (LPS), pokeweed mitogen (PWM), and polyinosinic-polycytidylic acid (PolyI:C) stimulation, respectively, and their expression level in anterior kidney and spleen was also increased after challenged with formalin-inactived Vibrio harveyi. These results indicated that humphead snapper CD79 molecule might play an important role in immune response to pathogen infection.

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Authors and Affiliations

  1. South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, P. R. China

    Yucong Huang

  2. University of Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Yucong Huang

  3. Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, P. R. China

    Yucong Huang, Xiuying Yan, Shuanghu Cai, Jia Cai, Jichang Jian, Yishan Lu & Jufen Tang

  4. Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals of Guangdong, Zhanjiang, 524088, P. R. China

    Yucong Huang, Xiuying Yan, Shuanghu Cai, Jia Cai, Jichang Jian, Yishan Lu, Jufen Tang & Zaohe Wu

  5. Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, P. R. China

    Zaohe Wu

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  1. Yucong Huang
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Huang, Y., Yan, X., Cai, S. et al. Characterization and expression analysis of B Cell receptor accessory molecule CD79 gene in humphead snapper (Lutjanus sanguineus). J. Ocean Univ. China 15, 318–326 (2016). https://doi.org/10.1007/s11802-016-2816-0

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