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RNA/DNA ratio and LPL and MyoD mRNA expressions in muscle ofOreochromis niloticus fed with elevated levels of palm oil

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Abstract

Palm oil is of great potential as one of the sustainable alternatives to fish oil (FO) in aquafeeds. In this present study, five isonitrogenous diets (32% crude protein) with elevated palm oil levels of 0%, 2%, 4%, 6% and 8% were used during an 8-week feeding trial to evaluate its effects on RNA/DNA ratio and lipoprotein lipase (LPL) and MyoD mRNA expressions in muscle of Oreochromis niloticus. The results showed that RNA, DNA content as well as ratio of RNA to DNA were significantly affected (P < 0.05), in each case the highest was recorded in fish group subjected to 6% palm oil level. There was a strong positive correlation between nucleic acid concentration (RNA concentration and RNA: DNA ratio) and specific growth rate (SGR), protein efficiency ratio (PER), while a negative correlation existed between nucleic acid concentration (RNA concentration and RNA: DNA ratio) and feed conversion ratio (FCR). The mRNA expressions of LPL and MyoD in muscle were not significantly affected by the different palm oil levels, although the highest expression was observed in fish fed with 6% palm oil level. There also existed a strong positive correlation between the mRNA expression of LPL, MyoD and SGR, PER, while their correlation with FCR was negative. In conclusion, elevated palm oil affected the RNA, DNA concentration as well as RNA/DNA ratio significantly, although the mRNA expression of LPL and MyoD were not affected significantly by elevated palm oil levels.

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  1. Key Laboratory of Freshwater Fishery Germplasm Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai, 201306, P. R. China

    Christian Larbi Ayisi & Jinliang Zhao

  2. L & A Aquaculture Research and Training Centre, P.O Box 2, Adukrom Akuapem, Eastern Region, Ghana

    Christian Larbi Ayisi

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Ayisi, C.L., Zhao, J. RNA/DNA ratio and LPL and MyoD mRNA expressions in muscle ofOreochromis niloticus fed with elevated levels of palm oil. J. Ocean Univ. China 15, 184–192 (2016). https://doi.org/10.1007/s11802-016-2752-z

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