Skip to main content
SpringerLink
Log in

Isolation of prawn (Exopalaemon carinicauda) lipopolysaccharide and β-1, 3-glucan binding protein gene and its expression in responding to bacterial and viral infections

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

The pattern recognition proteins (PRPs) play a major role in immune response of crustacean to resist pathogens. In the present study, as one of PRPs, lipopolysaccharide and β-1, 3-glucan binding protein (LGBP) gene in the ridge tail white prawn (Exopalaemon carinicauda) (EcLGBP) was isolated. The full-length cDNA of EcLGBP was 1338 bp, encoding a polypeptide of 366 amino acid residules. The deduced amino acid sequence of EcLGBP shared high similarities with LGBP and BGBP from other crustaceans. Some conservative domains were predicted in EcLGBP sequence. EcLGBP constitutively expressed in most tissues at different levels, and the highest expression was observed in hepatopancreas. With infection time, the cumulative mortality increased gradually followed by the proliferation of Vibrio parahaemolyticus and white spot syndrome virus (WSSV). The expression of EcLGBP in response to V. parahaemolyticus infection was up-regulated in hemocytes and hepatopancreas, and the up-regulation in hepatopancreas was earlier than that in hemocytes. EcLGBP expression after WSSV infection increased at 3 h, then significantly decreased in both hemocytes and hepatopancreas. The results indicated that EcLGBP was involved in the immune defense against bacterial and viral infections.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Akira, S., Takeda, K., and Kaisho, T., 2001. Toll-like receptors: Critical proteins linking innate and acquired immunity. Nature Immunology, 2(8): 675–680.

    Article  Google Scholar 

  • Akira, S., Uematsu, S., and Takeuchi, O., 2006. Pathogen recognition and innate immunity. Cell, 124(4): 783–801.

    Article  Google Scholar 

  • Amparyup, P., Sutthangkul, J., and Charoensapsri, W., 2012. Pattern recognition protein binds to lipopolysaccharide and ß-1, 3-glucan and activates shrimp prophenoloxidase system. The Journal of Biological Chemistry, 287(13): 10060–10069.

    Article  Google Scholar 

  • Bachère, E., 2000. Shrimp immunity and disease control. Aquaculture, 191: 3–11.

    Article  Google Scholar 

  • Cheng, W., Liu, C. H., Tsai, C. H., and Chen, J. C., 2005. Molecular cloning and characterization of a pattern recognition molecule, lipopolysaccharide-and ß-1, 3-glucan binding protein (LGBP) from the white shrimp Litopenaeus vannamei. Fish & Shellfish Immunology, 18(4): 297–310.

    Article  Google Scholar 

  • Christophides, G. K., Vlachou, D., and Kafatos, F. C., 2004. Comparative and functional genomics of the innate immune system in the malaria vector Anopheles gambiae. Immunological Reviews, 198(1): 127–148.

    Article  Google Scholar 

  • Dimopoulos, G., Richman, A., Müller, H. M., and Kafatos, F. C., 1997. Molecular immune responses of the mosquito Anopheles gambiae to bacteria and malaria parasites. Proceedings of the National Academy of Sciences, 94(21): 11508–11513.

    Article  Google Scholar 

  • Du, X. J., 2007. Pattern recognition and effectors of innate immunity in Chinese shrimp (Fenneropenaeus chinensis). PhD thesis. Shandong University, Jinan (in Chinese).

    Google Scholar 

  • Du, X. J., Zhao, X. F., and Wang, J. X., 2007. Molecular cloning and characterization of a lipo-polysaccharide and ß-1, 3-glucan binding protein from fleshy prawn (Fenneropenaeus chinensis). Molecular Immunology, 44: 1085–1094.

    Article  Google Scholar 

  • Duan, Y. F., Liu, P., Li, J., Li, J., Gao, B. Q., and Chen, P., 2013. cDNA cloning, characterization and expression analysis of peroxiredoxin 5 gene in the ridgetail white prawn Exopalaemon carinicauda. Molecular Biology Reports, 40(12): 6569–6577.

    Article  Google Scholar 

  • Duvic, B., and Söderhäll, K., 1992. Purification and partial characterization of a ß-1, 3-glucan-binding-protein membrane receptor from blood cells of the crayfish Pacifastacus leniusculus. European Journal of Biochemistry, 207(1): 223–228.

    Article  Google Scholar 

  • Dziarski, R., 2004. Peptidoglycan recognition proteins (PRGPs). Molecular Immunology, 40: 877–886.

    Article  Google Scholar 

  • Escobedo-Bonilla, C. M., Audoorn, L., Wille, M., Alday-Sanz, V., Sorgeloos, P., Pensaert, M. B., and Nauwynck, H. J., 2006. Standardized white spot syndrome virus (WSSV) inoculation procedures for intramuscular or oral routes. Diseases of Aquatic Organisms, 68: 181–188.

    Article  Google Scholar 

  • Figueroa-Pizano, M. D., Peregrino-Uriarte, A. B., Yepiz-Plascencia, G., Martínez-Porchas, M., Gollas-Galván, T., and Martínez-Córdova, L. R., 2014. Gene expression responses of white shrimp (Litopenaeus vannamei) infected with necrotizing hepatopancreatitis bacterium. Aquaculture, 420: 165–170.

    Article  Google Scholar 

  • Flegel, T. W., 2012. Historic emergence, impact and current status of shrimp pathogens in Asia. Journal of Invertebrate Pathology, 110(2): 166–173.

    Article  Google Scholar 

  • Flegel, T. W., Lightner, D. V, and Lo, C. F., 2008. Owens L. shrimp disease control: Past, present and future. Diseases in Asian Aquaculture, 4: 355–378.

    Google Scholar 

  • Ge, Q., Liang, J., Li, J., Li, J., Duan, Y., Zhao, F., and Ren, H., 2015. Molecular cloning and expression analysis of Relish gene from the ridgetail white prawn Exopalaemon carinicauda. Fisheries Science, 81(4): 699–711.

    Article  Google Scholar 

  • Gross, P. S., Bartlett, T. C., Browdy, C. L., Chapman, R. W., and Warr, G. W., 2001. Immune gene discovery by expressed sequence tag analysis of hemocytes and hepatopancreas in the Pacific white shrimp, Litopenaeus vannamei, and the Atlantic white shrimp, L. setiferus. Developmental & Comparative Immunology, 25(7): 565–577.

    Article  Google Scholar 

  • He, N. H., Qin, Q. W., and Xu, X., 2005. Differential profile of genes expressed in hemocytes of white spot syndrome virus-resistant shrimp (Penaeus japonicus) by combining suppression subtractive hybridization and differential hybridization. Antiviral Research, 66(1): 39–45.

    Article  Google Scholar 

  • Holmblad, T., Thörnqvist, P. O., Söderhäll, K., and Johanßson, M. W., 1997. Identification and cloning of an integrin ß subunit from hemocytes of the freshwater crayfish Pacifastacus leniusculus. Journal of Experimental Zoology, 277(3): 255–261.

    Article  Google Scholar 

  • Jiang, H., Cai, Y. M., Chen, L. Q., Zhang, X. W., Hu, S. N., and Wang, Q., 2009. Functional annotation and analysis of expressed sequence tags from the hepatopancreas of mitten crab (Eriocheir sinensis). Marine Biotechnology, 11(3): 317–326.

    Article  Google Scholar 

  • Kim, Y. S., Ryu, J. H., Han, S. J., Choi, K. H., Nam, K. B., Jang, I. H., and Lee, W. J., 2000. Gram-negative bacteria-binding protein, a pattern recognition receptor for lipopolysaccharide and ß-1, 3-glucan that mediates the signaling for the induction of innate immune genes in Drosophila melanogaster cells. Journal of Biological Chemistry, 275(42): 32721–32727.

    Article  Google Scholar 

  • Kwang, J., 2011. Oral vaccination of baculovirus-expressed VP28 displays enhanced protection against white spot syndrome virus in Penaeus monodon. PloS One, 6 (11): e26428.

    Article  Google Scholar 

  • Leaño, E. M., and Mohan, C. V., 2012. Early mortality syndrome threatens Asia’s shrimp farms. Global Aquaculture Advocate, 2012 (7/8): 38–39.

    Google Scholar 

  • Lee, S. Y., Wang, R., and Söderhäll, K., 2000. A lipopolysaccharide-and ß-1, 3-glucan-binding protein from hemocytes of the freshwater crayfish Pacifastacus leniusculus purification, characterization, and cDNA cloning. Journal of Biological Chemistry, 275(2): 1337–1343.

    Article  Google Scholar 

  • Li, F., Wang, D., Li, S., Yan, H., Zhang, J., Wang, B., and Xiang, J., 2010. A dorsal homolog (FcDorsal) in the Chinese shrimp Fenneropenaeus chinensis is responsive to both bacteria and WSSV challenge. Developmental & Comparative Immunology, 34(8): 874–883.

    Article  Google Scholar 

  • Lin, Y. C., Vaseeharan, B., and Chen, J. C., 2008. Identification and phylogenetic analysis on lipopolysaccharide and ß-1, 3-glucan binding protein (LGBP) of kuruma shrimp Marsupenaeus japonicus. Developmental & Comparative Immunology, 32(11): 1260–1269.

    Article  Google Scholar 

  • Livak, K. J., and Schmittgen, T. D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods, 25: 402–408.

    Article  Google Scholar 

  • Medzhitov, R., and Janeway, C., 2000. Innate immune recognition: Mechanisms and pathways. Immunological Reviews, 173(1): 89–97.

    Article  Google Scholar 

  • Roux, M. M., Pain, A., Klimper, K. R., and Dhar, A. Y., 2002. The lipopolysaccharide and ß-1, 3-glucan binding protein gene is upregulated in white spot virus-infected shrimp (Penaeus stylirostris). Journal of Virology, 76(14): 7140–7149.

    Article  Google Scholar 

  • Sánchez-Martínez, J. G., Aguirre-Guzmán, G., and Mejía-Ruiz, H., 2007. White spot syndrome virus in cultured shrimp: A review. Aquaculture Research, 38: 1339–1354.

    Article  Google Scholar 

  • Spann, K. M., and Lester, R. J. G., 1997. Viral diseases of penaeid shrimp with particular reference to four viruses recently found in shrimp from Queensland. World Journal of Microbiology and Biotechnology, 13(4): 419–426.

    Article  Google Scholar 

  • Takeuchi, O., and Akira, S., 2010. Pattern recognition receptors and inflammation. Cell, 140(6): 805–820.

    Article  Google Scholar 

  • Tamura, K., Dudley, J., Nei, M., and Kumar, S., 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8): 1596–1599.

    Article  Google Scholar 

  • Taßsanakajon, A., Somboonwiwat, K., Supungul, P., and Tang, S., 2013. Discovery of immune molecules and their crucial functions in shrimp immunity. Fish & Shellfish Immunology, 34: 954–967.

    Article  Google Scholar 

  • Thörnqvist, P. O., Johansson, M. W., and Söderhäll, K., 1994. Opsonic activity of cell adhesion proteins and ß-1, 3-glucan binding protein from two crustaceans. Developmental and Comparative Immunology, 18: 3–12.

    Article  Google Scholar 

  • Vargas, A. F., Jiménez, V. F., and Söderhäll, K., 1996. A plasma protein isolated from brown shrimp (Penaeus californiensis) which enhances the activation of prophenoloxidase system by ß-1, 3-glucan. Developmental and Comparative Immunology, 20: 299–306.

    Article  Google Scholar 

  • Vaseeharan, B., 2012. cDNA cloning, characterization and expreßsion of lipopolysaccharide and ß-1, 3-glucan binding protein (LGBP) gene from the Indian white shrimp Fenneropenaeus indicus. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 163(1): 74–81.

    Google Scholar 

  • Wang, B., Li, F., Luan, W., Xie, Y., Zhang, C., Luo, Z., and Xiang, J., 2008. Comparison of gene expression profiles of Fenneropenaeus chinensis challenged with WSSV and Vibrio. Marine Biotechnology, 10(6): 664–675.

    Article  Google Scholar 

  • Xia, D. Q., 1999. Biological characteristics and aquaculture technique of Exopalaemon carinicauda. China Fisheries, 3: 42–43 (in Chinese).

    Google Scholar 

  • Yan, D. C., Dong, S. L., Huang, J., and Zhang, J. S., 2007. White spot syndrome virus (WSSV) transmission from rotifer inoculum to crayfish. Journal of Invertebrate Pathology, 94: 144–148.

    Article  Google Scholar 

  • Yang, F., He, J., Lin, X., Li, Q., Pan, D., Zhang, X., and Xu, X., 2001. Complete genome sequence of the shrimp white spot bacilliform virus. Journal of Virology, 75(23): 11811–11820.

    Article  Google Scholar 

  • Yeh, M. S., Chang, C. C., and Cheng, W., 2009. Molecular cloning and characterization of lipopolysaccharide-and ß-1, 3-glucan-binding protein from the giant freshwater prawn Macrobrachium rosenbergii and its transcription in relation to foreign material injection and the molt stage. Fish & Shellfish Immunology, 27: 701–706.

    Article  Google Scholar 

  • Yeh, S. P., Chen, Y. N., Hsieh, S. L., Cheng, W., and Liu, C. H., 2009. Immune response of white shrimp, Litopenaeus vannamei, after a concurrent infection with white spot syndrome virus and infectious hypodermal and hematopoietic necrosis virus. Fish & Shellfish Immunology, 26(4): 582–588.

    Article  Google Scholar 

  • Zhang, Y., Jin, S., and Zhao, Q. S., 2010. Cloning and expreßsion pattern analysis of a lipopolysaccharide-and ß-1, 3-glucan-binding protein in Portunus trituberculatus. Zoological Research, 31(6): 587–594 (in Chinese).

    Google Scholar 

  • Zhao, D., Chen, L., Qin, C., Zhang, H., Wu, P., Li, E., and Qin, J., 2009. Molecular cloning and characterization of the lipopolysaccharide and ß-1, 3-glucan binding protein in Chinese mitten crab (Eriocheir sinensis). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 154(1): 17–24.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Fisheries College, Ocean University of China, Qingdao, 266003, P. R. China

    Qianqian Ge, Jitao Li & Ming Sun

  2. Key Laboratory for Sustainable Utilization of Marine Fisheries Resources of Ministry of Agriculture, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China

    Qianqian Ge, Jian Li, Ming Sun & Fazhen Zhao

  3. Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization of Ministry of Agriculture, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, P. R. China

    Yafei Duan

  4. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, P. R. China

    Qianqian Ge, Jian Li, Ming Sun & Fazhen Zhao

Authors
  1. Qianqian Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jian Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yafei Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jitao Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ming Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Fazhen Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jian Li.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ge, Q., Li, J., Duan, Y. et al. Isolation of prawn (Exopalaemon carinicauda) lipopolysaccharide and β-1, 3-glucan binding protein gene and its expression in responding to bacterial and viral infections. J. Ocean Univ. China 15, 288–296 (2016). https://doi.org/10.1007/s11802-016-2741-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-016-2741-2

Keywords

Search

Navigation