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Mapping toll-like receptor signaling pathway genes of Zhikong scallop (Chlamys farreri) with FISH

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Abstract

Toll-like receptor (TLR) signaling pathway plays a pivotal role in the innate immune system. Studies on TLR signaling pathway genes in Zhikong scallop (Chlamys farreri) have mainly focused on sequence analysis and expression profiling, no research has been carried out on their localization. The chromosomal position of TLR signaling pathway genes can be valuable for assemblying scallop genome and analysizing gene regulatory networks. In the present study, five key TLR signaling pathway genes (CfTLR, CfMyd88, CfTRAF6, CfNFκB, and CfIκB) containing bacterial artificial chromosomes (BACs) were isolated and physically mapped through fluorescence in situ hybridization on five non-homologous chromosome pairs, showing a similar distribution to another five model species. The isolation and mapping of these key immune genes of C. farreri will aid to the research on innate immunity, assignment of interested genes to chromosomes, and integration of physical, linkage and cytogenetic maps of this species.

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Authors and Affiliations

  1. Key Laboratory of Marine Genetics and Breeding of Ministry of Education, College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, P. R. China

    Bosong Zhao, Liang Zhao, Huan Liao, Jie Cheng, Shanshan Lian, Xuan Li, Xiaoting Huang & Zhenmin Bao

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  1. Bosong Zhao
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  2. Liang Zhao
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  3. Huan Liao
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  4. Jie Cheng
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  5. Shanshan Lian
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  6. Xuan Li
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  7. Xiaoting Huang
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  8. Zhenmin Bao
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Correspondence to Xiaoting Huang.

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Zhao, B., Zhao, L., Liao, H. et al. Mapping toll-like receptor signaling pathway genes of Zhikong scallop (Chlamys farreri) with FISH. J. Ocean Univ. China 14, 1075–1081 (2015). https://doi.org/10.1007/s11802-015-2643-8

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  • DOI: https://doi.org/10.1007/s11802-015-2643-8

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