Abstract
This study investigated the effect of dietary lipid on the growth, fatty acid composition and Δ5 fatty acyl desaturase genes (Fads) expression of juvenile abalone (Haliotis discus hannai Ino) hepatopancreas. Six purified diets were formulated to contain tripalmitin (TP), olive oil (OO, 72.87% 18:1n-9), grape seed oil (GO, 68.67% 18:2n-6), linseed oil (LO, 70.48% 18:3n-3), ARA oil (AO, 41.81% ARA) or EPA oil (EO, 44.09% EPA and 23.67% DAH). No significant difference in survival rate was observed among abalone fed with different diets. Weight gain rate (WGR) and daily growth rate of shell length (DGR SL) were significantly increased in abalone fed with diets containing OO, AO and EO, but decreased in abalone fed with LO diet (P < 0.05) in comparison with those fed with TP. High level of dietary 18:2n-6 resulted in higher content of n-6 polyunsaturated fatty acids (PUFAs) in abalone fed with GO than those fed with TP, OO, LO and EO (P < 0.05). n-3 PUFAs in abalone fed with LO was significantly higher than those in abalone fed with TP, OO, GO and AO (P < 0.05). The highest contents of 20:1n-9 and 22:1n-9 were observed in abalone fed with OO. The expression of Δ5 Fads in hepatopancreas of abalone was enhanced by high concentration of 18:3n-3 and suppressed by dietary LC-PUFAs; however it was not affected by dietary high concentration of 18:1n-9 or 18:2n-6. These results provided valuable information for understanding the synthesis of LC-PUFAs and nutritional regulation of Δ5 Fads expression in abalone.
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Agaba, M. K., Tocher, D. R., Zheng, X., Dickson, C. A., Dick, J. R., and Teale, A. J., 2005. Cloning and functional characterisation of polyunsaturated fatty acid elongases of marine and freshwater teleost fish. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 142: 342–352.
Ai, Q. H., Zhao, J. Z., Mai, K. S., Xu, W., Tan, B. P., Ma, H. M., and Liufu, Z. G., 2008. Optimal dietary lipid level for large yellow croaker (Pseudosciaena crocea) larvae. Aquaulture Nutrition, 14: 515–522.
Association of Official Analytical Chemists (AOAC), 1995. Official Methods of Analysis of Official Analytical Chemists International. 16th edition. Association of Official Analytical Chemists, Arlington, VA, USA.
Bahurmiz, O. M., and Ng, W. K., 2007. Effects of dietary palm oil source on growth, tissue fatty acid composition and nutrient digestibility of red hybrid tilapia, Oreochromis sp., raised from stocking to marketable size. Aquaculture, 262: 382–392.
Bautista-Teruel, M. N., Koshio, S. S., and Ishikawa, M., 2011. Diet development and evaluation for juvenile abalone, Haliotis asinina Linne: Lipid and essential fatty acid levels. Aquaculture, 312: 172–179.
Bell, J. G., Henderson, R. J., Tocher, D. R., McGhee, F., Dick, J. R., Porter, A., Smullen, R. P., and Sargent, J. R., 2002. Substituting fish oil with crude palm oil in the diet of Atlantic salmon (Salmo salar) affects hepatopancreas fatty acid composition and hepatic fatty acid metabolism. The Journal of nutrition, 132: 222–230.
Durazo-Beltrán, E., D’Abramo, L. R., Toro-Vazquez, J. F., Vasquez-Peláez, C., and Viana, M. T., 2003. Effect of triacylglycerols in formulated diets on growth and fatty acid composition in tissue of green abalone (Haliotis fulgens). Aquaculture, 224: 257–270.
González-Rovira, A., Mourente, G., Zheng, X., Tocher, D. R., and Pendón, C., 2009. Molecular and functional characterization and expression analysis of a Δ6 fatty acyl desaturase cDNA of European Sea Bass (Dicentrarchus labrax L.). Aquaculture, 298: 90–100.
Gregory, M. K., See, V. H., Gibson, R. A., and Schuller, K. A., 2010. Cloning and functional characterisation of a fatty acyl elongase from southern bluefin tuna (Thunnus maccoyii). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 155: 178–185.
Hastings, N., Agaba, M., Tocher, D. R., Leaver, M. J., Dick, J. R., Sargent, J. R., and Teale, A. J., 2001. A vertebrate fatty acid desaturase with Δ5 and Δ6 activities. Proceedings of the National Academy of Sciences, 98: 14304–14309.
Hastings, N., Agaba, M., Tocher, D., Zheng, X., Dickson, C., Dick, J., and Teale, A., 2004. Molecular cloning and functional characterization of fatty acyl desaturase and elongase cDNAs involved in the production of eicosapentaenoic and docosahexaenoic acids from α-linolenic acid in Atlantic salmon (Salmo salar). Marine Biotechnology, 6: 463–474.
Henderson, R., and Tocher, D. R., 1987. The lipid composition and biochemistry of freshwater fish. Progress in Lipid Research, 26: 281–347.
Izquierdo, M., Robaina, L., Juárez-Carrillo, E., Oliva, V., Hernández-Cruz, C. M., and Afonso, J. M., 2008. Regulation of growth, fatty acid composition and delta 6 desaturase expression by dietary lipids in gilthead seabream larvae (Sparus aurata). Fish Physiol Biochem, 34: 117–127.
Kamarudin, M., Ramezani-Fard, E., Saad, C., and Harmin, S., 2012. Effects of dietary fish oil replacement by various vegetable oils on growth performance, body composition and fatty acid profile of juvenile Malaysian mahseer, Tor tambroides. Aquaculture Nutrition, 18: 532–543.
Li, M. Z., Mai, K. S., Ai, Q. H., He, G., Xu, W., Zhang, W. B., Zhang, Y. J., and Zhou, H. H., 2013a. Effects of dietary grape seed oil and linseed oil on growth, muscle fatty acid composition and expression of putative Δ5 fatty acyl desaturase in abalone Haliotis discus hannai Ino. Aquaculture, 406–407: 105–114.
Li, M. Z., Mai, K. S., He, G., Ai, Q. H., Zhang, W. B., Xu, W., Wang, J., Liufu, Z. G., Zhang, Y. J., and Zhou, H. H., 2013b. Characterization of two Δ5 fatty acyl desaturases in abalone (Haliotis discus hannai Ino). Aquaculture, 416–417: 48–56.
Li, Y., Monroig, O., Zhang, L., Wang, S., Zheng, X., Dick, J. R., You, C., and Tocher, D. R., 2010. Vertebrate fatty acyl desaturase with Δ4 activity. Proceedings of the National Academy of Sciences, 107: 16840–16845.
Lim, P. K., Boey, P. L., and Ng, W. K., 2001. Dietary palm oil level affects growth performance, protein retention and tissue vitamin E concentration of African catfish, Clarias gariepinus. Aquaculture, 202: 101–112.
Livak, K. J., and Schmittgen, T. D., 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25: 402–408.
Mai, K., Mercer, J. P., and Donlon, J., 1995. Comparative studies on the nutrition of two species of abalone, Haliotis tuberculata L. and Haliotis discus hannai Ino. III. Response of abalone to various levels of dietary lipid. Aquaculture, 134: 68–80.
Mai, K., Mercer, J. P., and Donlon, J., 1996. Comparative studies on the nutrition of two species of abalone, Haliotis tuberculata L. and Haliotis discus hannai Ino. V. The role of polyunsaturated fatty acids of macroalgae in abalone nutrition. Aquaculture, 139: 77–89.
Mateos, H. T., Lewandowski, P. A., and Su, X. Q., 2011. Dietary fish oil supplements increase tissue n-3 fatty acid composition and expression of delta-6 desaturase and elongase-2 in Jade Tiger hybrid abalone. Lipids, 46: 741–751.
Mateos, H. T., Lewandowski, P. A., and Su, X. Q., 2012a. Effects of dietary fish oil replacement with flaxseed oil on tissue fatty acid composition and expression of desaturase and elongase genes. Journal of the Science of Food and Agriculture, 92: 418–426.
Mateos, H. T., Lewandowski, P. A., and Su, X. Q., 2012b. The effect of replacing dietary fish oil with canola oil on fatty acid composition and expression of desaturase and elongase genes in Jade Tiger hybrid abalone. Food Chemistry, 131: 1217–1222.
Matsuzaka, T., Shimano, H., Yahagi, N., Amemiya-Kudo, M., Yoshikawa, T., Hasty, A. H., Tamura, Y., Osuga, J. I., Okazaki, H., and Iizuka, Y., 2002. Dual regulation of mouse Δ5-and Δ6-desaturase gene expression by SREBP-1 and PPARα. Journal of Lipid Research, 43: 107–114.
Metcalfe, L. D., Schmitz, A. A., and Pelka, J. R., 1966. Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analytical Chemistry, 38: 514–515.
Mishra, K., and Samantaray, K., 2004. Interacting effects of dietary lipid level and temperature on growth, body composition and fatty acid profile of rohu, Labeo rohita (Hamilton). Aquaculture Nutrition, 10: 359–369.
Monroig, Ó., Navarro, J. C., Dick, J. R., Alemany, F., and Tocher, D. R., 2012. Identification of a Δ5-like fatty acyl desaturase from the cephalopod Octopus vulgaris (Cuvier 1797) involved in the biosynthesis of essential fatty acids. Marine Biotechnology, 14: 411–422.
Monroig, Ó., Zheng, X., Morais, S., Leaver, M. J., Taggart, J. B., and Tocher, D. R., 2010. Multiple genes for functional Δ6 fatty acyl desaturases (Fad) in Atlantic salmon (Salmo salar L.): Gene and cDNA characterization, functional expression, tissue distribution and nutritional regulation. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1801: 1072–1081.
Morais, S., Monroig, O., Zheng, X., Leaver, M. J., and Tocher, D. R., 2009. Highly unsaturated fatty acid synthesis in Atlantic salmon: Characterization of ELOVL5-and ELOVL2-like elongases. Marine Biotechnology, 11: 627–639.
Mourente, G., and Tocher, D. R., 1993. Incorporation and metabolism of 14 C-labelled polyunsaturated fatty acids in juvenile gilthead sea bream Sparus aurata L. in vivo. Fish Physiol Biochem, 10: 443–453.
Ng, W. K., Lim, P. K., and Boey, P. L., 2003. Dietary lipid and palm oil source affects growth, fatty acid composition and hepatopancreas α-tocopherol concentration of African catfish, Clarias gariepinus. Aquaculture, 215: 229–243.
Olsen, Y., 2011. Resources for fish feed in future mariculture. Aquaculture Environment Interactions, 1: 187–200.
Pickova, J., and Morkore, T., 2007. Alternate oils in fish feeds. European Journal of Lipid Science and Technology, 109: 256–263.
Portolesi, R., Powell, B. C., and Gibson, R. A., 2008. Δ6 desaturase mRNA abundance in HepG2 cells is suppressed by unsaturated fatty acids. Lipids, 43: 91–95.
Ren, H. T., Yu, J. H., Xu, P., and Tang, Y. K., 2012. Influence of dietary fatty acids on hepatopancreas fatty acid composition and expression levels of Δ6 desaturase-like and Elovl5-like elongase in common carp (Cyprinus carpio var. Jian). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 163: 184–92.
Riediger, N. D., Othman, R. A., Suh, M., and Moghadasian, M. H., 2009. A Systemic Review of the Roles of n-3 Fatty Acids in Health and Disease. Journal of the American Dietetic Association, 109: 668–679.
Rosenlund, G., Corraze, G., Izquierdo, M., and Torstensen, B. E., 2011. The effects of fish oil replacement on nutritional and organoleptic qualities of farmed fish. In: Fish Oil Replacement and Alternative Lipid Sources in Aquaculture Feeds. Turchini, G. M., et al., eds., Boca Raton, FL, USA: CRC Press; Taylor and Francis group, 487–522.
Sales, J., and Janssens, G., 2004. Use of feed ingredients in artificial diets for abalone: A brief update. Nutrition Abstracts and Reviews: Series B, 74: 13N–21N.
Seiliez, I., Panserat, S., Corraze, G., Kaushik, S., and Bergot, P., 2003. Cloning and nutritional regulation of a Δ6-desaturase-like enzyme in the marine teleost gilthead seabream (Sparus aurata). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 135: 449–460.
Seiliez, I., Panserat, S., Kaushik, S., and Bergot, P., 2001. Cloning, tissue distribution and nutritional regulation of a Δ6-desaturase-like enzyme in rainbow trout. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 130: 83–93.
Smink, W., Gerrits, W., Gloaguen, M., Ruiter, A., and van Baal, J., 2011. Linoleic and α-linolenic acid as precursor and inhibitor for the synthesis of long-chain polyunsaturated fatty acids in liver and brain of growing pigs. Animal, 1: 1–9.
Su, X., Antonas, K., and Li, D., 2004. Comparison of n-3 polyunsaturated fatty acid contents of wild and cultured Australian abalone. International Journal of Food Sciences and Nutrition, 55: 149–154.
Tocher, D. R., 2003. Metabolism and functions of lipids and fatty acids in teleost fish. Reviews in Fisheries Science, 11: 107–184.
Tocher, D. R., and Ghioni, C., 1999. Fatty acid metabolism in marine fish: Low activity of fatty acyl Δ5 desaturation in gilthead sea bream (Sparus aurata) cells. Lipids, 34: 433–440.
Tocher, D. R., Zheng, X., Schlechtriem, C., Hastings, N., Dick, J. R., and Teale, A. J., 2006. Highly unsaturated fatty acid synthesis in marine fish: Cloning, functional characterization, and nutritional regulation of fatty acyl Δ6 desaturase of Atlantic cod (Gadus morhua L.). Lipids, 41: 1003–1016.
Vagner, M., and Santigosa, E., 2011. Characterization and modulation of gene expression and enzymatic activity of delta-6 desaturase in teleosts: A review. Aquaculture, 315: 131–143.
Xu, W., Mai, K. S., Zhang, W. B., Liufu, Z. G., Tan, B. P., Ma, H. M., and Ai, Q. H., 2004. Influence of dietary lipid sources on growth and fatty acid composition of juvenile abalone, Haliotis discus hannai Ino. Journal of Shellfish Research, 127: 29–40.
Zheng, X., Ding, Z., Xu, Y., Monroig, O., Morais, S., and Tocher, D. R., 2009. Physiological roles of fatty acyl desaturases and elongases in marine fish: Characterisation of cDNAs of fatty acyl Δ6 desaturase and elovl5 elongase of cobia (Rachycentron canadum). Aquaculture, 290: 122–131.
Zheng, X., Tocher, D. R., Dickson, C. A., Bell, J. G., and Teale, A. J., 2004. Effects of diets containing vegetable oil on expression of genes involved in highly unsaturated fatty acid biosynthesis in liver of Atlantic salmon (Salmo salar). Aquaculture, 236: 467–483.
Zheng, X., Tocher, D. R., Dickson, C. A., Bell, J. G., and Teale, A. J., 2005a. Highly unsaturated fatty acid synthesis in vertebrates: New insights with the cloning and characterization of a Δ6 desaturase of Atlantic salmon. Lipids, 40: 13–24.
Zheng, X., Torstensen, B. E., Tocher, D. R., Dick, J. R., Henderson, R. J., and Bell, J. G., 2005b. Environmental and dietary influences on highly unsaturated fatty acid biosynthesis and expression of fatty acyl desaturase and elongase genes in liver of Atlantic salmon (Salmo salar). Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1734: 13–24.
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Li, M., Mai, K., Ai, Q. et al. Effect of dietary lipid on the growth, fatty acid composition and Δ5 Fads expression of abalone (Haliotis discus hannai Ino) hepatopancreas. J. Ocean Univ. China 14, 317–324 (2015). https://doi.org/10.1007/s11802-015-2356-z
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DOI: https://doi.org/10.1007/s11802-015-2356-z