Abstract
This study examined the effect of salinity on the expression of Na+/K+-ATPase (NKA) α-subunit and vacuolar-type H+-ATPase (V-ATPase) β-subunit gene in the gill of Litopenaeus vannamei. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay showed that the expression of NKA α-subunit and V-ATPase β-subunit gene was significantly influenced by salinity. It was found that the NKA activity significantly varied with salinity in time and dose dependent manner; whereas the V-ATPase activity did not. The abundance of NKA α-subunit gene transcript increased rapidly when the salinity decreased from 26b to 21, and slowly when the salinity decreased from 26 to 31 within the first 24 h. When the salinity decreased from 26 to 21, the transcription of NKA α-subunit gene in gill epithelium was higher at 12 h than that at 0 h, which was consistent with the result of immunoblotting assay of NKA α-subunit. In addition, salinity had a significant time- and dose-dependent effect on the concentration of biogenic amines in both hemolymph and gill. As compared to other parameters, the concentration of dopamine (DA) and 5-hydroxytryptamine (5-HT) varied in different patterns when the salinity decreased from 26 to 21 or increased from 26 to 31, suggesting that DA and 5-HT played different regulatory roles in osmotic adaption and modulation of shrimp when salinity varies.
Similar content being viewed by others
References
Babili, G., and Zwingolstein, G., 1996. Does sphingomyelin participant in signal transduction in the gill cells of euryhaline crabs during salinity changes. Comparative Biochemistry and Physiology, 115(1): 7–12.
Carlos, M. L., Dirk, W., Mihaela, S., and David, W. T., 2005. Induction of branchial ion transporter mRNA expression during acclimation to salinity change in the euryhaline crab Chasmagnathus granulatus. The Journal of Experimental Biology, 208: 3627–3636.
Castiho, P. C., Martins, I. A., and Bianchini, A., 2001. Gill Na+/K+-ATPase and osmoregulation in the estuarine crab, Chasmagnathus granulata Dana, 1851 (Decapoda, Grapsidae). Journal of Experimental Marine Biology and Ecology, 256: 215–227.
Chiu, H. T., Yeh, S. P., Huang, S. H., Chang, C. C., Kuo, C. M., and Cheng, W., 2006. Dopamine induces transient modulation of the physiological responses of whiteleg shrimp, Litopenaeus vannamei. Aquaculture, 251: 558–566.
Freire, C. A., Mcnamara, J. C., Rosa, J. C., and Greene, L. J., 1995. Neuroendocrine control of osmotic regulation in the freshwater shrimp Macrobrachium olfersii (Wiegmann) (Crustacea, Decapoda): Free amino acid concentrations in the hemolymph. General and Comparative Endocrinology, 100: 83–91.
Freire, C. A., Onken, H., and McNamara, J. C., 2008. A structure-function analysis of ion transport in crustacean gill and excretory organs. Comparative Biochemistry and Physiology, Part A, 151: 272–304.
Gong, H., Jiang, D. H., Lightner, D. V., Collins, C., and Brock, D., 2004. A dietary modification approach to improve the osmoregulatory capacity of Litopenaeus vannamei cultured in the Arizona desert. Aquaculture Nutrition, 10: 227–236.
Halperin, J., Genovese, G., Tresguerres, M., and Luquet, C. M., 2004. Modulation of ion uptake across posterior gill of the crab Chasmagnathus granulatus by dopamine and cAMP. Comparative Biochemistry and Physiology, Part A, 139: 103–109.
Kazuyuki, H., and Shigehisa, H., 2007. Expression of endocrine genes in zebrafish larvae in response to environmental salinity. Journal of Endocrinology, 193: 481–491.
Kumlu, M., and Eroldogan, O. T., 2000. Effects of temperature and salinity on larval growth survival and development of Penaeus semisulcatus. Aquaculture, 188: 167–173.
Liu, H. Y., Pan, L. Q., and Zheng, D. B., 2009. Effects of injection of biogenic amines on expression of gill related ion transporter mRNA and α-subunit protein in Litopenaeus vannamei. Comparative Biochemistry and Physiology, Part A, 154: 29–36.
Lovett, D. L., and Watts, S. A., 1995. Changes in polyamine levels in response to acclimation salinity in gill of the blue crab Callinectes sapidus. Comparative Biochemistry and Physiology, 110B: 115–119.
Lucu, C., and Devescovi, M., 1999. Osmoregulation and branchial Na+/K+-ATPase in the lobster Homarus gammarus acclimated to dilute seawater. Journal of Experimental Marine Biology and Ecology, 234: 291–304.
Matthew, B. J., 1975. Synergistic effects of salinity, temperature and heavy metals on mortality and osmoregulation in marine and estuarine isopods (Crustacea). Marine Biology, 30: 13–20.
Mo, J. L., Devos, P., and Trausch, G., 1998. Dopamine as a modulator of ionic transport and Na+/K+-ATPase activity in the gill of the Chinese crab Eriocheir sinensis. Journal of Crustacean Biology, 18: 442–448.
Mo, J. L., Devos, P., and Trausch, G., 2003. Active absorption of Cl− and Na+ in posterior gill of chinese crab, Eriocheir sinensis: Modulation by dopamine and cAMP. Journal of Crustacean Biology, 23: 505–512.
Morris, S., 2001. Neuroendocrine regulation of osmoregulation and the evolution of air-breathing in decapod crustaceans. The Journal of Experimental Biology, 204: 979–989.
Morris, S., and Edwards, T., 1995. Control of osmoregulation via regulation of Na+/K+-ATPase activity in the amphibious purple shore crab Leptograpsus variegates. Comparative Biochemistry and Physiology, 112C: 129–136.
Onken, H., and Putzenlechner, M., 1995. V-ATPase drives active, electrogenic and Na super(+)-independent Cl super(−) absorption across the gill of Eriocheir sinensis. The Journal of Experimental Biology, 198(3): 767–774.
Onken, H., and Riestenpatt, S., 1998. NaCl absorption across split gill lamellae of hyperregulating crabs: Transport mechanisms and their regulation. Comparative Biochemistry and Physiology, 119A: 883–893.
Pan, L. Q., Zhang, L. J., and Liu, H. Y., 2007. Effects of salinity and pH on ion-transport enzyme activities, survival and growth of Litopenaeus vannamei postlarvae. Aquaculture, 273: 711–720.
Péqueux, A., 1995. Osmotic regulation in crustaceans. Journal of Crustacean Biology, 15: 1–60.
Péqueux, A., Bras, P. L., Cann-Moisan, C., Caroff, J., and Sébert, P., 2002. Polyamines, indolamines, and catecholamines in gill and haemolymph of the euryhaline crab, Eriocheir sinensis. Effects of high pressure and salinity. Crustaceana, 75: 567–578.
Riestenpatt, S., Petrausch, G., and Siebers, D., 1995. Cl super(-) influx across posterior gill of the Chinese crab (Eriocheir sinensis): Potential energization by a V-type H super(+)-ATPase. Comparative Biochemistry and Physiology, Part A, 110A(3): 235–241.
Robsertson, L., Bray, W., Leung-Trujillo, J., and Lawrence, A., 1987. Practical molt staging of Penaeus setiferus and Penaeus stylirostris. Journal of the World Aquaculture Society, 18: 180–185.
Sommer, M. J., and Mantel, L. H., 1991. Effects of dopamine and acclimation to reduced salinity on the concentration of cyclic AMP in the gill of the green crab, Carcinus maenas (L). General and Comparative Endocrinology, 82: 364–368.
Saoud, I. P., Davis, D. A., and Rouse, D. B., 2003. Suitability studies of inland well waters for Litopenaeus vannamei culture. Aquaculture, 217: 373–383.
Simonetta, L., Paolo, E., Piero, G. G., Romina, M., and Enrico, A. F., 2005. Role of biogenic amines and cHH in the crustacean hyperglycemic stress response. The Journal of Experimental Biology, 208: 3341–3347.
Spanings-Pierrot, C., Soyez, D., Van Herp, F., Gompel, M., Skaret, G., Grousset, E., and Charmantier, G., 2000. Involvement of crustacean hyperglycemic hormone in the control of gill ion transport in the crab Pachygrapsus marmoratus. General and Comparative Endocrinology, 119: 340–350.
Tierney, A. J., Kim, T., and Abrams, R., 2003. Dopamine in crayfish and other crustaceans: Distribution in the central nervous system and physiological functions. Microscopy Research and Technique, 60: 325–335.
Towle, D. W., Paulsen, R. S., Weihrauch, D., Kordylewski, M., Salvador, C., Lignot, J. H., and Spanings-Pierrot, C., 2001. Na+/K+-ATPase in gill of the blue crab Callinectes sapidus: cDNA sequencing and salinityrelated expression of α-subunit mRNA and protein. Journal of Experimental Biology, 204: 4005–4012.
Towle, D. W., Rushton, M. E., Heidysch, D., Magnani, J. J., Rose, M. J., Amstutz, A., Jordan, M. K., Shearer, D. W., and Wu, W. S., 1997. Sodium/proton antiporter in the euryhaline crab Carcinus maenas: Molecular cloning, expression and tissue distribution. Journal of Experimental Biology, 200(6): 1003–1014.
Trausch, G., Forget, M. C., and Devosm, P., 1989. Bioamines-stimulated phosphorylation and Na+/K+-ATPase in the gill of Chinese crabs, Eriocheir sinensis. Comparative Biochemistry and Physiology, 94B: 487–492.
Weihrauch, D., Ziegler, A., Siebers, D., and Towle, D. W., 2002. Active ammonia excretion across the gill of the green shore crab Carcinus maenas: Participation of Na+/K+-ATPase, V-type H+-ATPase and functional microtubules. The Journal of Experimental Biology, 205: 2765–2775.
Zatta, P., 1987. Dopamine, noradrenaline and serotonin during hypoosmotic stress of Carcinus maenas. Marine Biology, 96: 479–481.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Pan, L., Liu, H. & Zhao, Q. Effect of salinity on the biosynthesis of amines in Litopenaeus vannamei and the expression of gill related ion transporter genes. J. Ocean Univ. China 13, 453–459 (2014). https://doi.org/10.1007/s11802-014-2013-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11802-014-2013-y