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Structure and anti-influenza A (H1N1) virus activity of three polysaccharides from Eucheuma denticulatum

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Abstract

Three polysaccharides (EW, EH and EA) were prepared from a red alga Eucheuma denticulatum by sequential extraction with cold water, hot water and sodium hydroxide water solution. Their monosaccharide compositions, relative molecular mass and structural characterization were determined by gas chromatography, high performance 1iquid chromatography, fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy methods. EW was hybrid ı/κ/ν-carrageenan (70 ı/17κ/13ν-carrabiose), EH was mainly ı-carrageenan, and EA was mainly α-1,4-Glucan (88%) but mixed with small amount of ı-carrageenan (12%). The relative molecular mass of EW, EH and EA was 480, 580 and 510 kDa, respectively. The anti-influenza A (H1N1) virus activity of these three polysaccharides was evaluated using the Madin-Darby canine kidney cells model. EW showed good anti-H1N1 virus activity, its IC50 was 276.5 μg mL−1, and the inhibition rate to H1N1 virus was 52% when its concentration was 250 μg mL−1. The IC50 of ı-carrageenan EH was 366.4 μg mL−1, whereas EA showed lower anti-H1N1 virus activity (IC50>430 μg mL−1). Available data obtained give positive evidence that the hybrid carrageenan EW from Eucheuma denticulatum can be used as potential anti-H1N1 virus inhibitor in future.

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Authors and Affiliations

  1. Key Laboratory of Marine Drugs, Ministry of Education, Qingdao, 266003, P. R. China

    Guangli Yu, Miaomiao Li, Youjing Lv, Guangsheng Li, Guangling Jiao & Xia Zhao

  2. Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, P. R. China

    Guangli Yu, Miaomiao Li, Wei Wang, Xin Liu, Xiaoliang Zhao, Youjing Lv & Xia Zhao

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  1. Guangli Yu
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  2. Miaomiao Li
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  3. Wei Wang
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  4. Xin Liu
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  5. Xiaoliang Zhao
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  7. Guangsheng Li
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  8. Guangling Jiao
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  9. Xia Zhao
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Correspondence to Guangli Yu.

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Yu, G., Li, M., Wang, W. et al. Structure and anti-influenza A (H1N1) virus activity of three polysaccharides from Eucheuma denticulatum . J. Ocean Univ. China 11, 527–532 (2012). https://doi.org/10.1007/s11802-012-2107-3

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  • DOI: https://doi.org/10.1007/s11802-012-2107-3

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