Abstract
Three kinds of polysaccharides: GFW, GFH and GFA, were sequentially extracted from a red alga Gloiopeltis furcata with 25°C and 85°C water, and 60 4% NaOH water solution. Based on the defatted alga, the yields of the polysaccharide were 57.9%, 2.5% and 2.6%, respectively. Their monosaccharide compositions, average molecular weights and structural characters were determined by gas chromatography (GC), high performance liquid chromatography (HPLC), fourier transform infrared spectroscopy (FTIR) or 13C-NMR spectroscopy. The results showed that GFW, GFH and GFA were all composed of D-galactose (Gal) and 3,6-anhydro-L-galactose (AnG), and particularly GFA also contained xylose (Xyl). The average molecular weights of GFW, GFH and GFA were 22.6 kD, 26.5 kD and 49.8 kD, respectively, with the respective sulfate content 31.2%, 25.1% and 22.7%. The data of FTIR and 13C-NMR confirmed the sulfate ester location at C6 of galactose. It is concluded that all the three polysaccharides extracted from Gloiopeltis furcata were sulfated galactans, two being sulfated-agarose, and one being xylose-containing sulfated galactan.
Similar content being viewed by others
References
Dodgson, K. S., and Price, R. G., 1962. A note on the determination of the ester sulfate content of sulfated polysaccharides. J. Biol. Chem., 84: 106–110.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F., 1956. Colorimetric method for determination of sugar and related substances. Anal. Biochem., 28: 350–356.
Hirase, S., and Araki, C., 1958. Isolation of agarobiose derivative from the mucilage of Gloiopeltis furcata. Bull. the Chem. Soc. Japn., 30: 68–70.
Izumi, K., 1973. Structural analysis of agar-type polysaeeharides by NMR spectroscopy. Biochim. Biophys. Acta, 320(2): 311–317.
Ji, M. H., and Gu, K. N., 1987. The 13C-NMR Analysis of funoran of Gloiopeltis furcata and carrageenan of Eucheuma gelatinae. Mar. Sci., 2: 50–52 (in Chinese with English abstract).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., 1951. Protein measurement with the Folin Phenol reaction. J. Biol. Chem., 193(1): 265–275.
Niu, R. L., Fan, X., and Han, L. J., 2003. A screening for the anti-inflammatory effect of algal extracts. Oceanol. Limnol. Sin., 34(2): 150–154 (in Chinese with English abstract).
Quemener, B., and Lahaye, M., 1998. Comparative analysis of sulfated galactans from red algae by reductive hydrolysis and mild methanolysis coupled to two different HPLC techniques. J. Appl. Phycol, 10(1): 75–81.
Song, J. B., and Yung, H. C., 2007. Methanol extract of the seaweed Gloiopeltis furcata induces G2/M arrest and inhibits cyclooxygenase-2 activity in human hepatocarcinoma HepG2 cells. Phytother. Res., 21: 52–57.
Stevenson, T. T., and Furneaux, R. H., 1991. Chemical methods for the analysis of sulphated galactans from red algae. Carbohydr. Res., 210: 277–298.
Wei, Y. N., and Fang, J. N., 1989. Determination of purity and molecular weight of polysaccharides by high performance gel permeation chromatography. Acta Pharm. Sin., 24(7): 532–536 (in Chinese with English abstract).
Yang, B., Yu, G., Zhao, X., Jiao, G., Ren, S., and Chai, W., 2009. Effect of the 3,6-Anhydro-Form of Galactose on Mild Acid Hydrolysis of Polysaccharides Carrageenan and Agarose. FEBS J., 276: 2125–2137.
Yaphe, W., and Arsenault, G. P., 1965. Improved resorcinol reagent for the determination of fructose, and of 3, 6-anhydro-galactose in polysaccharides. Anal. Biochem., 13: 143–148
Yin, L. M., Wei, Y. L., and Ding, W., 1988. Screening marine aigae from Qingdao coast for antitumor and antibacterial activity. Chin. J. Mar. Drugs, 7: 39–41 (in Chinese with English abstract).
Yu, G., Yang, B., Ren, W., Zhao, X., Zhang, J., Barrow, C., 2007. A comparative analysis of four kinds of polysaccharides purified from Furcellaria lumbricalis. J. Ocean Univ. Chin., 6(1): 16–20.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yu, G., Hu, Y., Yang, B. et al. Extraction, isolation and structural characterization of polysaccharides from a red alga Gloiopeltis furcata . J. Ocean Univ. China 9, 193–197 (2010). https://doi.org/10.1007/s11802-010-0193-7
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11802-010-0193-7