Skip to main content

Antibacterial activity of extracts from Zostera marina against pathogens of Apostichopus japonicus skin ulceration disease

Abstract

The purpose of this study was to investigate the antibacterial activity of extracts from Zostera marina against the pathogens of Apostichopus japonicus skin ulceration disease. When 95% ethanol (v/v) solvent was used to extract Zostera marina at 50°C, aqueous extract (ZA) showed obvious bacteriostatic effects on the tested bacterial strains (inhibition halo diameters between 8.23 mm and 13.62 mm), whereas the ethyl acetate extract (ZE) was almost inactive. The minimal inhibitory concentration (MIC) of ZA against four pathogens were homogeneous at 12.8 g L−1. ZA components were analyzed by thin layer chromatography (TLC) assay and six fractions were obtained. In another study, the six fractions showed inhibitory effects against the tested bacteria while their functions seemed to counteract the ZA activity.

This is a preview of subscription content, access via your institution.

References

  • Davis, J., 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science, 264: 375–382.

    Article  Google Scholar 

  • Hentzer, M., Riedel, K., and Rasmussen, T. B., 2002. Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology, 148: 87–102.

    Google Scholar 

  • Lin, X. P., Zhang, Y., and Zhang, X. Q., 2005. Antimicrobial activities of ethanol extracts from three brown algae against the phytopathogenic microorganisms and animal pathogenic bacteria. J. Fujian Normal Univ., 21(1): 112–116.

    Google Scholar 

  • Liu, Y. G., and Wang, G. H., 2006. The effect of the Zostera marina in the rearing of Apostichopus japonicus. Shandong Fish., 23(4):12.

    Google Scholar 

  • MA, H. Y., and Jiang, G. L., 2006. Microscopic and pathogenic research of reared Stichopus japonica suffers from a kind of skin ulceration disease. Master Thesis. Ocean University of China, Qingdao.

    Google Scholar 

  • Murray, P. R., Baron, E. J., and Pfaller, M. A., 1995. Manual of Clinical Microbiology. Mosby Year Book, London, 78–84.

    Google Scholar 

  • Robles, C. P. O., and Ballantine, D. L., 1996. Dynamics of antibacterial activity in three species of Caribbean marine algae as a function of habitat and life history. Hydrobiologia, 326/327: 457–462.

    Article  Google Scholar 

  • Robles, C. P. O., and Ballantine, D. L., 1999. Effects of culture conditions on production of antibiotically active metabolites by the marine alga Spyridea filamentosal (Ceramiaceae, Rhodophyta). J. Appl. Phycol., 11: 217–224.

    Article  Google Scholar 

  • Service, R. F., 1995. Antibiotics that resist resistance. Science, 270: 724–727.

    Article  Google Scholar 

  • Xu, N. J., and Fan, X., 2002. Study on the antibacterial activities of the ethanol of the seaweed. Oceanol. Limnol. Sin., 33(3): 265–270.

    Google Scholar 

  • Zhang, C. Y., Wang, Y. G., and Rong, X. J., 2004. Natural resources, culture and problems of sea cucumber worldwide. Mar. Fish. Res., 25(3): 89–97.

    Google Scholar 

  • Zhang, Y., and Jiang, G. L., 2009. Quorum sensing research of dominant microflora of Apostichopus japonica suffers from the skin ulceration syndrom. Master Thesis. Ocean University of China, Qingdao.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Guoliang Jiang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Liu, Y., Jiang, G. & Wu, Z. Antibacterial activity of extracts from Zostera marina against pathogens of Apostichopus japonicus skin ulceration disease. J. Ocean Univ. China 9, 68–70 (2010). https://doi.org/10.1007/s11802-010-0068-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-010-0068-y

Key words

  • Zostera marina
  • Apostichopus japonicus skin ulceration disease
  • antibacterial activity