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Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck)

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Abstract

The pathological changes of hemocytes in the haemolymph and hepatopancreas were examined in experimentally and naturally WSSV (white spot syndrome virus) infected Fenneropenaeus chinensis. The results showed that the pathological manifestations of hemocytes were similar among moribund shrimps infected via injection, feeding and by nature. Firstly, the total hemocyte counts (THCs) in WSSV-infected shrimp were significantly lower than those in healthy shrimp. Secondly, necrotic, broken and disintegrated cells were often observed, and a typical hematolysis was present in the haemolymph smear of WSSV-infected shrimp. Thirdly, necrosis and typical apoptosis of hemocytes were detected with TEM in the peripheral haemolymph of WSSV-infected shrimp. Hyalinocytes and semi-granulocytes with masses of WSSVs in their nuclei often appeared, whereas no granular hemocytes with WSSV were found in the hepatopancreas of moribund infected shrimps. All our results supported that hemocytes were the main target cells of WSSV, and hyalinocytes and semigranular hemocytes seemed to be more favorable for WSSV infection in F. chinensis.

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References

  • Chakraborty, A., S. K. Otta, B. Joseph, S. Kumar, M. S. Hossain, I. Karunasagar, et al., 2002. Prevalence of white spot syndrome virus in wild crustaceans along the coast of India. Curr. Sci., 82(11):1392–1397.

    Google Scholar 

  • Chang, P. S., H. C. Chen, and Y. C. Wand, 1998. Detection of white spot syndrome associated baculovirus WSBV in experimentally infected wild shrimps crabs and lobsters by in-situ hybridization. Aquaculture, 164: 23–43.

    Article  Google Scholar 

  • Evelyne, B., 2003. Shrimp immunity and disease control. Aquculture, 191: 3–11.

    Google Scholar 

  • Feng, S. M., X. L. Yang, J. Li, R. Wang, and Y. C. Zhao, 2006. Haemolymph pathological research on white spot syndrome (WSS) of Litopenaeus vannamei. J. Fish. Chin., 30(2): 108–112 (in Chinese with English abstract).

    Google Scholar 

  • Granja, C. B., L. F. Aranguren, O. M. Vidal, L. Aragon, and M. Salazar, 2003. Does hyperthermia increase apoptosis in white spot syndrome virus (WSSV)-infected Litopenaeus vannamei? Dis. Aquat. Org., 54: 13–18.

    Article  Google Scholar 

  • Hu, Y. B., Y. Wang, and N. B. Jiang, 2005. Effects of ammonia-N and nitrite-N on the hemocyte count and ultrastructure of Macrobrachium rosenbergii. J. Zhejiang Univ. (Science Edition), 32: 691–697 (in Chinese with English abstract).

    Google Scholar 

  • Jiang, Y. S., W. B. Zhan, S. B. Wang, and J. Xing, 2006. Development of primary shrimp hemocyte cultures of Penaeus chinensis to study white spot syndrome virus (WSSV) infection. Aquaculture, 253: 114–119.

    Article  Google Scholar 

  • Jiravanichpaisal, P., S. Sricharoen, I. Söderhäll, and K. Söderhäll, 2006. White spot syndrome virus (WSSV) interaction with crayfish haemocytes. Fish Shellfish Immunol., 20(5): 718–727.

    Article  Google Scholar 

  • Khanobdee, K., C. Soowannayan, T. W. Flegel, S. Ubol, and B. Withyachumnarnkul, 2002. Evidence for apoptosis correlated with mortality in the giant black tiger shrimp Penaeus monodon infected with yellow head virus. Dis. Aquat. Org., 48: 79–90.

    Article  Google Scholar 

  • Lightner, D. V., K. W. Hasson, B. L. White, and R. M. Redman, 1998. Experimental infection of western hemisphere penaeid shrimp with Asian white spot syndrome virus and Asian yellow head virus. J. Aquat. Anim. Health, 10: 271–281.

    Article  Google Scholar 

  • Lo, C. F., J. H. Leu, C. H. Ho, C. H. Chen, S. E. Peng, Y. T. Chen, et al., 1996. Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using poly-merase chain reaction. Dis. Aquat. Org., 25: 133–141.

    Article  Google Scholar 

  • Mialhe, E., E. Bachère, V. Boulo, and J. P. Cadoret, 1995. Strategy for research and international cooperation in marine invertebrate pathology, immunology and genetics. Aquaculture, 132: 3341.

    Article  Google Scholar 

  • Needham, A. E., 1970. Haemostatic mechanisms in the invertebrata. In: Symposia of the Zoological Society of London. Maefarlane, R.G., et al., eds., Academic Press, London, 19–14.

    Google Scholar 

  • Office International des epizooties (OIE), 2000. Diagnostic Manual for Aquatic Animal Diseases. 3rd edition, Agriculture Technology Publishing Company, Beijing, 222–227.

    Google Scholar 

  • Pantoja, C. R., and D. V. Lightner, 2003. Similarity between the histopathology of white spot syndrome virus and yellow head syndrome virus and its relevance to diagnosis of YHV disease in the Americas. Aquaculture, 218: 47–54.

    Article  Google Scholar 

  • Rajendran, K. V., K. K. Vijayan, T. C. Santiagoand, and J. J. S. Rajan, 2005. White spot syndrome virus (WSSV) infection in tiger shrimp Penaeus monodon: A non-lethal histopathological rapid diagnostic method using paraffin and frozen sections. Aquac. Int., 13: 341–349.

    Article  Google Scholar 

  • Sahtout, A., M. D. Hassan, M. Shariff, 2001. DNA fragmentation, an indicator of apoptosis, in cultured black tiger shrimp Penaeus monodon infected with white spot syndrome virus (WSSV). Dis. Aquat. Org., 44: 155–159.

    Article  Google Scholar 

  • Sahul, H. A. S., M. Sarathi, R. Sudhakaran, G. Balasubramanian, M. S. Syed, 2006. Quantitative assessment of apoptotic hemocytes in white spot syndrome virus (WSSV)-infected penaeid shrimp, Penaeus monodon and Penaeus indicus, by flow cytometric analysis. Aquaculture, 256: 111–120.

    Google Scholar 

  • Sarathi, M., V. P. I. Ahmed, C. Venkatesan, G. Balasubramanian, J. Prabavathy, and H. A. S. Sahul, 2007. Comparative study on immune response of Fenneropenaeus indicus to Vibrio alginolyticus and white spot syndrome virus. Aquaculture, 271: 8–20.

    Article  Google Scholar 

  • Smith, V. J., and P. A. Johnston, 1992. Differential haemotoxic effect of PCB congeners in the common shrimp, Crangon crangon. Comp. Biochem. Physiol., 101C: 641–649.

    Google Scholar 

  • Supamattaya, K., S. Kiriratnikom, M. Boonyaratpalin, and L. Borowitzka, 2005. Effect of a Dunaliella extract on growth performance, health condition, immune response and disease resistance in black tiger shrimp (Penaeus monodon). Aquaculture, 248: 207–216.

    Article  Google Scholar 

  • Wang, C. H., K. F. J. Tang, G. H. Kou, and S. N. Chen, 1997. Light and electron microscopic evidence of white spot disease in the giant tiger shrimp, Penaeus monodon (Fabricius), and the kuruma shrimp, Penaeus japonicus (Bate), cultured in Taiwan. J. Fish Dis. 203: 23–321.

    Google Scholar 

  • Wang, Y. C., C. F. Lo, P. S. Chang, and G. H. Kou, 1998. Experimental infection of white spot baculovirus in some cultured and wild decapods in Taiwan. Aquaculture, 164: 221–231.

    Article  Google Scholar 

  • Wang, Y. T., W. Liu, J. N. Seah, C. S. Lam, J. H. Xiang, V. Korzh, et al., 2002. White spot syndrome virus (WSSV) infects specific hemocytes of the shrimp Penaeus merguiensis. Dis. Aquat. Org., 52: 249–259.

    Article  Google Scholar 

  • Wongprasert, K., K. Khanobdee, S. S. Glunukarnl, P. Meeratana, and B. Withyachumnarnkull, 2003. Time-course and levels of apoptosis in various tissues of black tiger shrimp Penaeus monodon infected with white-spot syndrome virus. Dis. Aquat. Org., 55: 3–10.

    Article  Google Scholar 

  • Wu, J. L., and K. Muroga, 2004. Apoptosis does not play an important role In the resistance of ‘immune’ Penaeus japonicus against white spot syndrome virus. J. Fish Dis., 27: 15–21.

    Article  Google Scholar 

  • Yoganandhan, K., S. Thirupathi., and A. S. S. Hameed, 2003. Biochemical, physiological and hematological changes in white spot syndrome virus-infected shrimp. Penaeus indicus. Aquaculture, 221: 1–11.

    Article  Google Scholar 

  • Zhan, W. B., Y. H. Wang, K. K. Yu, H. Fukuda, and Q. X. Meng, 1998. White spot syndrome virus infection of cultured shrimp in China. J. Aquat. Anim. Health, 10: 405–410.

    Article  Google Scholar 

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Authors and Affiliations

  1. Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao, 266003, P. R China

    Shouming Feng, Wenbin Zhan & Jing Xing

  2. Tianjin Fishery Institute, Tianjin, 300221, P. R. China

    Shouming Feng, Jun Li, Kai Yang & Jing Wang

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  1. Shouming Feng
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  2. Wenbin Zhan
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  3. Jing Xing
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  4. Jun Li
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  5. Kai Yang
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  6. Jing Wang
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Correspondence to Wenbin Zhan.

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Feng, S., Zhan, W., Xing, J. et al. Hematological changes in white spot syndrome virus-infected shrimp, Fenneropenaeus chinensis (Osbeck). J. Ocean Univ. China 7, 287–293 (2008). https://doi.org/10.1007/s11802-008-0287-7

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  • DOI: https://doi.org/10.1007/s11802-008-0287-7

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