Skip to main content
SpringerLink
Log in

Histopathological observation of lymphocystis disease and lymphocystis disease virus (LCDV) detection in cultured diseased Sebastes schlegeli

  • Published:
Journal of Ocean University of China Aims and scope Submit manuscript

Abstract

Lymphocystis nodules occurring in the cultured sting fish Sebastes schlegeli were observed under light and electron microscope. Lymphocystis disease virus (LCDV) in the tissues of diseased fish was detected with indirect immunofluorescence test (IFAT). Results showed that lymphocystis cells had overly irregular nuclei, basophilic intracytoplasmic inclusion bodies with virions budding from the surface, and hyaline capsules outside the cell membrane. Numerous virus particles about 200 nm in diameter scattered in the cytoplasm, electron-dense particles 70–80 nm in diameter filled in perinuclear cisterna, and membrane-enveloped particles with electron-dense core of 70–80 nm appeared around cellular nucleus. IFAT using monoclonal antibody against LCDV from Paralichthys olivaceus revealed that specific green fluorescence was present in the cytoplasm of lymphocystis cells, epithelium of stomach, gill lamellae, and muscular fibers under epidermis of S. schlegeli, just as that in the cytoplasm of lymphocystis cells of P. olivaceus, suggesting the presence of LCDV in these tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anders, K., 1989. Lymphocystis disease in fishes. In: Viruses of lower vertebrates. Ahne W. and Kurstdk D., eds., Springer, Heidelbag, 141–160.

    Google Scholar 

  • Cheng, S.F., W. B. Zhan, J. Xing, and X. Z. Sheng, 2006. Development and characterization of monoclonal antibody to the lymphocystis disease virus of Japanese flounder Paralichthys olivaceus isolated from China. J. Virol. Methods, 135: 173–180

    Article  Google Scholar 

  • Colorni, A., and A. Diament, 1995. Splenic and cardiac lymphocystis in the red drum, Sciaenops ocellatus (L.). J. Fish Dis., 18: 467–471.

    Article  Google Scholar 

  • Garcia-Rosado, E., D. Castro, I. Cano, S. J. Perez-Prieto, and J. J. Borrego, 2002. Serological techniques for detection of lymphocystis virus in fish. Aquat. Living Resour., 15: 179–185.

    Article  Google Scholar 

  • Goorha, R. M., 1989. Frog virus 3 DNA replication. In: Viruses of lower vertebrates. Ahne W. and Kurstdk D., eds., Springer, Heidelbag, pp. 30–38.

    Google Scholar 

  • LaPatra, S. E., K. A. Roberti., J. S. Rohovec, and J. L. Fryer, 1989. Fluorescent antibody test for the rapid diagnosis of infectious hematopoietic necrosis. J. Aquat. Anim. Health, 1: 29–36.

    Article  Google Scholar 

  • Sano, T., H. Fukuda, and T. Komatsu, 1994. Thermal effect on lymphocystis of flounder (Paralichthys olivaceus). In: International symposium on aquatic animal health: program and abstract. University of California, School of Veterinary Medicine, Davis, CA (USA), 4–8.

    Google Scholar 

  • Sheng, X. Z., and W. B. Zhan, 2004. Occurrence, development and histochemical characteristics of lymphocystis in cultured Japanese flounder (Paralichthys Olivaceus). High Technol. Letters, 10(2): 92–96.

    Google Scholar 

  • Sheng, X. Z., and W. B. Zhan, 2006. Histopathological studies on target organs of lymphocystis disease virus of fish. J. Ocean Univ. China, 36(5): 749–753 (in Chinese with English abstract).

    Google Scholar 

  • Sun, X. Q., L. Y. Qu, and J. X. Zhang, 2000. Pathogenicity and immunogenicity of lymphocystis virus of Japanese flounder (Paralichthys olivaceus). High Technol. Letters, 9: 19–21 (in Chinese with English abstract).

    Google Scholar 

  • Tanaka, M., M. Yoshimizu, M. Kusakari, and T. Kimura, 1984. Lymphocystis disease in Sebastes schlegeli and hirame Paralichthys olivaceus in hokkaido, Japan. Bull. Jap. Soc. Sci. Fish., 50: 37–42.

    Google Scholar 

  • Tidona, C. A., and G. Darai, 1997. Molecular anatomy of lymphocystis disease virus. Arch. Virol., 13: 49–56.

    Google Scholar 

  • Wolf K., 1988. Fish viruses and fish viral disease, Cornell Univ. Press, New York, 268–291.

    Google Scholar 

  • Xu, S. J., J. Xing, S. F. Cheng, and W. B. Zhan, 2004. Detection of lymphocystis disease virus in flounder tissues by immune technique. J. Fish. China, 28: 101–105 (in Chinese with English abstract).

    Google Scholar 

  • Zhang, Q. Y., F. Xiao, J. Xie, Z. Q. Li, and J. F. Gui, 2004. Complete genome sequence of lymphocystis disease virus isolated from China. J. Virol., 78(13): 6982–6994.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Pathology and Immunology of Aquatic Animals, LMMEC, Ocean University of China, Qingdao, 266003, P. R. China

    Sheng Xiuzhen, Zhan Wenbin, Xu Songjuan & Cheng Shunfeng

Authors
  1. Sheng Xiuzhen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhan Wenbin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xu Songjuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Cheng Shunfeng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhan Wenbin.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sheng, X., Zhan, W., Xu, S. et al. Histopathological observation of lymphocystis disease and lymphocystis disease virus (LCDV) detection in cultured diseased Sebastes schlegeli . J Ocean Univ. China 6, 378–382 (2007). https://doi.org/10.1007/s11802-007-0378-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11802-007-0378-x

Key words

Search

Navigation