Abstract
The microsatellite-enriched library was constructed using magnetic bead hybridization selection method, and the microsatellite DNA sequences were analyzed in Pacific abalone Haliotis discus hannai. Three hundred and fifty white colonies were screened using PCR-based technique, and 84 clones were identified to potentially contain microsatellite repeat motif. The 84 clones were sequenced, and 42 microsatellites and 4 minisatellites with a minimum of five repeats were found (13.1% of white colonies screened). Besides the motif of CA contained in the oligoprobe, we also found other 16 types of microsatellite repeats including a dinucleotide repeat, two tetranucleotide repeats, twelve pentanucleotide repeats and a hexanucleotide repeat. According to Weber (1990), the microsatellite sequences obtained could be categorized structurally into perfect repeats (73.3%), imperfect repeats (13.3%), and compound repeats (13.4%). Among the microsatellite repeats, relatively short arrays (<20 repeats) were most abundant, accounting for 75.0%. The largest length of microsatellites was 48 repeats, and the average number of repeats was 13.4. The data on the composition and length distribution of microsatellites obtained in the present study can be useful for choosing the repeat motifs for microsatellite isolation in other abalone species.
Similar content being viewed by others
References
Bentzen, P., J. B. Olsen, J. E. McLean, T. R. Seamons, and T. P. Quinn, 2001. Kinship analysis of Pacific salmon: Insights into mating, homing, and timing of reproduction. J. Hered., 92: 127–136.
Brinkmann, B., M. Klintschar, F. Neuhuber, J. Hoehne, and R. Burkhard, 1998. Mutation rate in human microsatellites: Influence of the structure and length of the tandem repeat. Am. J. Hum. Genet., 62: 1408–1415.
Brooker, A. L., D. Cook, P. Bentzen, J. M. Wright, and R. W. Doyle, 1994. Organization of microsatellites differs between mammals and cold-water teleost fishes. Canad. J. Fish. Aquatic Sci., 51: 1959–1966.
Bull, L. N., C. R. Pabon-Pena, and N. B. Freimer, 1999. Compound microsatellite repeats: practical and theoretical features. Genome Res., 9: 830–838.
Edwards, A., A. Civitello, H. A. Hammond, and C. T. Caskey, 1991. DNA typing and genetic mapping with trimeric and tetrameric tandem repeats. Am. J. Hum. Genet., 49: 746–756.
Ellegren, H., 2002. Microsatellite evolution: a battle between replication slippage and point mutation. Trends Genet., 18: 70.
Evans, B., J. Bartlett, N. Sweijd, P. Cook, and N.G. Elliott, 2004. Loss of genetic variation at microsatellite loci in hatchery produced abalone in Australia (Haliotis rubra) and South Africa (Haliotis midae). Aquaculture, 233: 109–127.
Evans, B., R. W. White, and N. G. Elliott, 2000. Characterization of microsatellite loci in the Australian blacklip abalone (Haliotis rubra, Leach). Mol. Ecol., 9: 1183–1184.
Gardner, M. G., S. J. B. Cooper, C. M. Bull, and W. N. Grant, 1999. Isolation of microsatellite loci from a social lizard, Egernia stokesii, using a modified enrichment procedure. J. Hered., 90: 301–304.
Garza, J. C., and N. B. Freimer, 1996. Homoplasy for size at microsatellite loci in humans and chimpanzees. Genome Res., 6: 211–217.
Holland, B. S., 2001. Invasion without a bottleneck: Microsatellite variation in natural and invasive populations of the brown mussel Perna perna (L). Mar. Biotechnol., 3: 407–415.
Huang, B., and P. J. Hanna, 1998. Identification of three polymorphic microsatellite loci in blacklip abalone, Haliotis rubra (Leach), and detection in other abalone species. J. Shellfish Res., 17: 795–799.
Hubert, S., and D. Hedgecock, 2004. Linkage maps of microsatellite DNA markers for the Pacific oyster Crassostrea gigas. Genetics, 168: 351–362.
Kijas, J. M. H., J. C. S. Fowler, C. A. Garbett, and M. R. Thomas, 1994. Enrichment of microsatellites from the citrus genome using biotinylated oligonucleotide sequences bound to streptavidin-coated magnetic particles. Biotechniques, 16: 657–662.
Kirby, V. L., R. Villa, and D. A. Powers, 1998. Identification of microsatellites in the California red abalone, Haliotis rufescens. J. Shellfish Res., 17: 801–804.
Knapik, E. W., A. Goodman, M. Ekker, M. Chevrette, J. Delgado, et al., 1998. A microsatellite genetic linkage map for zebrafish (Danio rerio). Nat. Genet., 18: 338–343.
Kruglyak, S., R. T. Durrett, M. D. Schug, and C. F. Aquadro, 1998. Equilibrium distributions of microsatellite repeat length resulting from a balance between slippage events and point mutations. Proc. Natl. Acad. Sci. USA, 95: 10774–10778.
Li, Q. F., X. B. Zhao, and X. L. Luo, 2004. Construction and identification on enriched microsatellite library from yak genome. Acta Genetica Sinica, 31: 489–494 (in Chinese).
Li, Q., 2004. Advances in studies on microsatellite markers in the Pacific abalone. J. Ocean Univ. Chin., 34: 365–370 (in Chinese).
Li, Q., and A. Kijima, 2004. Rapid isolation and characteristic analysis of microsatellite clones in the Pacific oyster. Oceanol. Limnol. Sinica, 35: 364–370 (in Chinese).
Miller, K. M., K. Laberee, K. H. Kaukinen, S. Li, and R. E. Withler, 2001. Development of microsatellite loci in pinto abalone (Haliotis kamtschatkana). Mol. Ecol. Notes, 1: 315–317.
Naciri, Y., Y. Vigouroux, J. Dallas, E. Desmarais, C. Delsert, et al., 1995. Identification and inheritance of (GA/TC)n and (AC/GT)n repeats in the European flat oyster Ostrea edulis (L.). Mol. Mar. Biol. Biotechnol., 4: 83–89.
Norris, A., D. G. Bradley, and E. P. Cunningham, 2000. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture, 182: 73–83.
Nugroho, E., and N. Taniguchi, 1999. Isolation of greater amberjack microsatellite DNA and their application as genetic marker to species of genus Seriola from Japan. Fisheries Sci., 65: 353–357.
Ostrander, E. A., P. M. Jong, J. Rine, and G. Duyk, 1992. Construction of small-insert genomic DNA libraries highly enriched for microsatellite repeat sequences. Proc. Natl. Acad. Sci. USA, 89: 3419–3423.
Refseth, U. H., B. M. Fangan, and K. S. Jakobsen, 1997. Hybridization capture of microsatellites directly from genomic DNA. Electrophoresis, 18: 1519–1523.
Rico, C., K. M. Ibrahim, I. Rico, and G. M. Hewitt, 1997. Stock composition in North Atlantic populations of whiting using microsatellite markers. J. Fish Biol., 51: 462–475.
Roy, C. B., M. A. Nazeer, and T. Saha, 2004. Identification of simple sequence repeats in rubber (Hevea brasiliensis). Curr. Sci., 87: 807–811.
Sakamoto, T., R. G. Danzmann, K. Gharbi, P. Howard, A. Ozaki, et al., 2000. A microsatellite linkage map of rainbow trout (Oncorhynchus mykiss) characterized by large sex-specific differences in recombination rates. Genetics, 155: 1331–1345.
Sekino, M., and M. Hara, 2001. Microsatellite DNA loci in Pacific abalone Haliotis discus discus (Mollusca, Gastropoda, Haliotidae). Mol. Ecol. Notes, 1: 8–10.
Selvamani, M. J. P., S. M. Degnan, D. Paetkau, and B. M. Degnan, 2000. Highly polymorphic microsatellite loci in the Heron Reef population of the tropical abalone Haliotis asinina. Mol. Ecol., 9: 1184–1186.
Sokolov, E. P., I. M. Sokolova, and H. O. Portner. 2001. Composition and relative abundance of microsatellite repeats in genome of Littorina saxatilis (Olivi) (Gastropoda: Littorinidae). J. Moll. Stud., 67: 499–501.
Walsh, P. S., N. J. Fildes, and R. Reynolds, 1996. Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA. Nucleic Acids Res., 24: 2807–2812.
Weber, J. L., 1990. Informativeness of human (dC-dA)n (dG-dT)n polymorphisms. Genomics, 7: 524–530.
Xu, P., L. H. Zhou, and J. H. Xiang, 2001. Isolating microsatellite DNA of Chinese shrimp Penaeus chinensis. Oceanol. Limnol. Sinica, 32: 255–259.
Zane, L., L. Bargelloni, and T. Patarnello, 2002. Strategies for microsatellites isolation: a review. Mol. Ecol., 11: 1–16.
Zhang, Q., S. K. Jr. Allen, and K. S. Reece, 2005. Genetic variation in wild and hatchery stocks of Suminoe oyster (Crassostrea ariakensis) assessed by PCR-RFLP and microsatellite markers. Mar. Biotechnol., 7: 588–599.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, Q., Akihiro, K. Sequences characterization of microsatellite DNA sequences in Pacific abalone (Haliotis discus hannai). J Ocean Univ. China 6, 47–52 (2007). https://doi.org/10.1007/s11802-007-0047-0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11802-007-0047-0