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Determination of selenium in marine aquatic products by hydride generation-atomic fluorescence spectrometry (HG-AFS)

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Abstract

A method for the analysis of selenium in marine aquatic products by HG-AFS has been investigated. The method is based on the reduction of inorganic selenium to volatile SeH2 which is bubbled out by carrier gas of pure argon, and then swept to Ar-H2 flame quarts atomizer to measure its fluorescence intensity. The hydride generation, transportation, atomization and some instrumental parameters were studied by a kind of orthogonal design. The optimum conditions selected are as follows: reactive acidity, 20% HC1; the amount of NaBH4, 4.9mL; gas flow of argon, 6OO mLmin−1; atomizing temperature, 200°C, negative high voltage,−300V; light current, 100 mA; integral time, 7 s. The detection limit of the presented method is 0.072µgL−1 for selenium. The calibration curve shows a satisfactory line in the concentration range from 0.000 to l.OOOµgL−1 Se. The recovery is 95.8%–102.2% .

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Authors and Affiliations

  1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Minato, 108-8477, Tokyo, Japan

    Zhang Zhaohui, Tashiro Yuri & Hiroo Ogawa

  2. Department of Food Engineering, Ocean University of China, 266003, Qingdao, P.R.China

    Zhang Zhaohui & Gao Xin

Authors
  1. Zhang Zhaohui
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  2. Gao Xin
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  3. Tashiro Yuri
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  4. Hiroo Ogawa
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Correspondence to Zhang Zhaohui.

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Zhang, Z., Gao, X., Yuri, T. et al. Determination of selenium in marine aquatic products by hydride generation-atomic fluorescence spectrometry (HG-AFS). J Ocean Univ. China 4, 252–256 (2005). https://doi.org/10.1007/s11802-005-0043-1

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  • DOI: https://doi.org/10.1007/s11802-005-0043-1

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