Abstract
Water-soluble algal polysaccharides (APS) (alginic acid, fucoidan and laminaran) possess many pharmacological activities. The results of this study showed that the APS-Ce4+ complexes have some enzyme-like activities. Fucoidan and its complex with Ce4+ have activities similar to those of SOD. The activities of laminaran, alginic acid and their complexes are not measurable. The APS do not show measurable activities in the digestion of plasmid DNA. In contrast, the APS - Ce4+ complexes show these measurable activities under the comparable condition when APS bind Ce4+ and form homogenous solutions. The laminaran - Ce4+ complex shows the most obvious activity in the digestion of plasmid DNA, pNPP and chloropy-rifos under neutral conditions.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chen, X.H., X.M. Luo, and Y.C. Song, 1998. Hydrolysis of methionine-containing peptides in binuclear and mononuclear palladium (U) complexes. Polyhedron, 17 (13-14): 2271–2278.
Fan, X., S.C. Zhang, S. Qin, and X.J. Yan, 1999. Advances on Marine Biotechnology. China Ocean Press, Beijing, 182–188.
Hasan, H. M., 1984. Determination of microbial damage caused by oxygen free radical, and the protective rule of superoxide dismutase. In: Methods in Enzymology. Packer, L., et al., ed. Academic Press, New York, 105, 404 -4412.
He, S. G., X. L. Huang, and J. R. Fu, 2001. Molecular properties of polyamine oxidase from cowper primary leaves. Acta Phytophysiologica Sinica, 27(5): 425–430.
Horton, N.C., K.J. Newberry, and J. Perona, 1998. Metal ion-mediated substrate-assisted catalysis in type I restriction endonucleases. Proc. Natl. Acad. Sci. USA, 95: 13489–13494.
Jiang, X. L., S. Q. Liu, Z. H. Zhang, and H. S. Guan, 1999. Effects of polysaccharides on immunological activities of Penaeus chinens. Journal of Fishery Sciences of China, 6(1): 66–68.
Kajimura, A., J. Sumaoka, and M. Komiyama, 1998. DNA hydrolysis by cerium (IV )-saccharide complexes. Carbohydrate Research, 309: 305–351.
Kimura, E., and T. Koike, 1997. Intrinsic properties of zinc (II) ion pertinent to zinc enzymes. In: Advances in Inorganic Chemistry. Sykes, A.G., and Sam, D. eds., Academic Press, 44, 229–261.
Komiyama, M., 1995. Sequence-specific and hydrolytic scission of DNA and RNA by lanthanide complex-oligo DNA hybrids. Biochem., 118: 665–670.
Li, Z. J., C. H. Xue, L. Chen, Y. Fang, and H. Lin, 2001. Scavenging effects of fucoidan fractions of low molecular weight extracted from Laminaria japonica on radicals of active oxygen and antioxidation in vivo. Journal of Fisheries of China, 25(1): 64–68.
Liu, H., and G. Y. Li, 1998. The effect of immunopoly-saccharide as a food additive on the penaeid sharimp, Penaeus Vanname. Oceanologia et Limnologia Sinica, 29(3): 113–119.
Liu, W., J. Fang, and P.J. Gao, 1998. Purification and characterization of soybeen hull peroxidase. Chinese Journal of Biochemistry and Melecular Biology, 14(5): 576–582.
Sumaoka, J., T. Igawa, K. Furuki, and M. Komiyama, 2000. Homogeneous Ce (IV) complexes for efficient hydrolysis of plasmid DNA. Chemistry Letter, 56–57.
Tatiana, N. Z., M. S. Nataliya, B. P. Irina, B. P. Irina, and V.I. Vladimir, 1999. A new procedure for the separation of water-soluble polysaccharides from brown seaweeds. Carbohydrate Research, 322: 32–39.
Tatiana, N. P., and M. K. Nenad, 1996. New selectivity and turnoverin peptide hydrolysis by metal complexes. A palladium (II) aqua complex catalyzes cleavage of peptides next to the histidine residue. J. Am. Chem. Soc, 118: 51 -58.
Takarada, T., M. Yashira, and M. Komiyama, 2000. Catalytic hydrolysis of peptides by cerium (IV). Chem. Eur.J., 6(21): 3906–3913.
Wang, D. F., and F. D. Lu, 1997. Basis Experiment and Studies on Tea Biochemistry. Science, Technology and Document Press, Beijing, 16–21, 66–68.
Wu, D.R., 1987. Saccharides Biochemistry. High Education Press, Beijing, 582–583.
Xue, C.H., G.L. Yu, T. Hirata, M. Sakaguchi, and J. Terao, 1998a. Antioxidative activity of carp blood plasma on lipid peroxidation. Biosci. Biotechnol. Biochem., 62(2): 201–205.
Xue, C.H., G.L. Yu, T. Hirata, M. Sakaguchi, J. Terao, et al., 1998b. Antioxidative activities of several marine polysaccharides evaluated in a phosphatidylcholine-liposomal suspension and organic solvents. Biosci. Biotechnol. Biochem., 62(2): 206–209.
Xue, C. H., Y. Fang, H. Lin, C. Lei, Z. J. Li, et al., 2001. Chemical characters and antioxidative properties of sulfated polysaccharides from Laminaria japonica. J. Applied Phycol, 13(1): 67–70.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Wang, D., Sun, J., Du, D. et al. Enzyme-like activities of algal polysaccharide - cerium complexes. J Ocean Univ. China 4, 29–33 (2005). https://doi.org/10.1007/s11802-005-0020-8
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11802-005-0020-8