Abstract
Thirty-nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP, IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram. They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good for some special enzymes, such as LDH, EST, MPI, etc. Twenty-six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao sample (P<0.99). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017, while those of the Rizhao sample are 0.023 and 0.025 respectively.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Buth, D. G., 1992. Genetic principles and the interpretation of electrophoretic data. In: Electrophoretic and Electro Focusing Technique in Fisheries Management. D. H. Whitmore, ed., CRC Press, Florida, 23–84.
Carvalho, G. R., and Ch. M. Nigmatullin, 1998. Stock structure analysis and species identification. In: Squid Recruitment Dynamics. P. G. Rodhouse, et al., eds., FAO Fish Tech. Pap., Rome, 199–232.
Carvalho, G.R., and T.J. Pitcher, 1989. Biochemical genetic studies on the Patagonian squid, Loligo gahi d ’Orbigny. II. Population structure in Falkland waters using isozymes, morphometrics, and life history date. J. Exp. Biol. Ecol., 126: 243–258.
Carvalho, G. R., A. Thompson, and A. L. Stoner, 1992. Genetic diversity and population differentiation of the shortfin squid Illex angentinus in the south-west Atlantic. J. Exp. Mar. Biol. Ecol., 158: 105–121.
Dong, Z. Z., 1987. Fauna Sinica, Phylum Mollusca-Class Cephalopoda. Committee of Fauna Sinica. Academia Sinica, eds., Science Press, Beijing, 111–114.
Dong, Z.Z., 1991. Biology of the Economic Species of Cephalopods in the World Oceans. Shandong Science and Technology Press, Jinan, 216–227.
Hughes, C. R., and D. C. Queller, 1993. Detection of highly polymorphic microsatellite loci in a species with little allozyme polymorphism. Mol. Ecol., 2: 131–137.
Li, J. Y., 1963. On the breeding and migration of the golden cuttlefish, Sepia esculenta Hoyle, Living in the Yellow Sea. J. Shandong College of Oceanology, 2:69–108.
May, B., 1992. Starch gel electrophoresis of isozymes. In: Molecular Genetic Analysis of Populations: A Practical Approach. A. R. Hoelzel, ed., IRL Press, Oxford, 1–27; 271–280.
Natsukari, Y., S. Hirata, and M. Washizaki, 1991. Growth and seasonal change of shell characters of Sepia esculenta. In: The Cuttlefish, 1st International Symposium on the Cuttlefish Sepia. E. Boucaud, ed., Publication Center, University of Caen, Caen, 49–67.
Nei, M., 1987. Molecular Evolutionary Genetics. Columbia Univ. Press, New York, 128–134.
Nesis, Kir N., 1987. Cephalopods of the World. T. F. H. Publications, Inc., Ltd., Neptune city, 109–110.
Okutani, T., 1995. Cuttlefish and Squids of the World in Color. Published by National Cooperative Association of Squid Processors, Tokyo, 43 pp.
Perez-losada, M., A. Guerra, and A. Sanjuan, 1996. Allozyme electrophoretic technique and phylogenetic relationship in three species of Sepia (Cephalopoda: Sepi-idae). Comp. Biochem. Physiol., 114B(1): 11–18.
Shaklee, J.B., F.W. Allendorf, D.C. Morizot., and G.S. Whitt, 1990. Gene nomenclature for protein-coding loci in fish. Transactions of the American Fisheries Society, 119:2–15.
Zhang, G. F., and Zhang, F. S., 1993. Genetic diversity and sustainable utilization of marine shellfish. Mar. Sci., (5): 17–21.
Zheng, X.D., Y. Natsukari, Wang, R.C., Wang, Z. P., and Li, Y., 2001a. Biochemical genetic studies on cuttlefish Sepiella maindroni de Rochebrune, 1884 (Cephalopoda: Sepiidae) — Active loci screening of isozyme in S. maindroni. Oceanologia et limnologia sinica, 46(4): 345–355.
Zheng, X.D., Wang, R.C., and Wang, Z.P., 2001b. Advances of studies on Cephalopoda genetic variation. J. Fisheries of China, 25(1): 84–89.
Zheng, X.D., Wang, R.C., Wang, X.F., Xiao, S., and Chen, B., 2001c. Genetic variation in populations of the common Chinese cuttlefish Sepiella maindroni (Mollusca: Cephalopoda) using allozymes and mitochondrial DNA sequence analysis. J. Shellfish Res., 20(3): 1159–1165.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zheng, X., Zhao, J., Xiao, S. et al. Isozymes analysis of the golden cuttlefish Sepia esculenta (Cephalopoda: Sepiidae). J Ocean Univ. China 3, 48–52 (2004). https://doi.org/10.1007/s11802-004-0007-x
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11802-004-0007-x