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Microalgae cultivation and culture medium recycling by a two-stage cultivation system

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Abstract

Nutrients and water play an important role in microalgae cultivation. Using wastewater as a culture medium is a promising alternative to recycle nutrients and water, and for further developing microalgae-based products. In the present study, two species of microalgae, Chlorella sp. (high ammonia nitrogen tolerance) and Spirulina platensis (S. platensis, high growth rate), were cultured by using poultry wastewater through a two-stage cultivation system for algal biomass production. Ultrafiltration (UF) or centrifuge was used to harvest Chlorella sp. from the first cultivation stage and to recycle culture medium for S. platensis growth in the second cultivation stage. Results showed the two-stage cultivation system produced high microalgae biomass including 0.39 g·L–1Chlorella sp. and 3.45 g·L–1S. platensis in the first-stage and second-stage, respectively. In addition, the removal efficiencies of NH4+ reached 19% and almost 100% in the first and the second stage, respectively. Total phosphorus (TP) removal reached 17% and 83%, and total organic carbon (TOC) removal reached 55% and 72% in the first and the second stage, respectively. UF and centrifuge can recycle 96.8% and 100% water, respectively. This study provides a new method for the combined of pure microalgae cultivation and wastewater treatment with culture medium recycling.

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References

  • Abelson P H, Hoering T C (1961). Carbon isotope fractionation in formation of amino acids by photosynthetic organisms. Proceedings of the National Academy of Sciences of the United States of America, 47(5): 623–632

    CAS  Google Scholar 

  • Bhave R, Kuritz T, Powell L, Adcock D (2012). Membrane-based energy efficient dewatering of microalgae in biofuels production and recovery of value added co-products. Environmental Science & Technology, 46(10): 5599–5606

    CAS  Google Scholar 

  • Bilad M R, Arafat H A, Vankelecom I F J (2014). Membrane technology in microalgae cultivation and harvesting: A review. Biotechnology Advances, 32(7): 1283–1300

    CAS  Google Scholar 

  • Bilad M R, Discart V, Vandamme D, Foubert I, Muylaert K, Vankelecom I F (2014a). Coupled cultivation and pre-harvesting of microalgae in a membrane photobioreactor (MPBR). Bioresource Technology, 155: 410–417

    CAS  Google Scholar 

  • Canizares R O, Dominguez A R (1993). Growth of Spirulina maxima on swine waste. Bioresource Technology, 45(1): 73–75

    CAS  Google Scholar 

  • Chang Y, Wu Z, Bian L, Feng D, Leung Y C D (2013). Cultivation of Spirulina platensis for biomass production and nutrient removal from synthetic human urine. Applied Energy, 102: 427–431

    CAS  Google Scholar 

  • Cheryan M (1998). Ultrafiltration and Microfiltration Handbook. CRC Press: Boca Raton, FL.

    Google Scholar 

  • Dassey A J, Theegala C S (2013). Harvesting economics and strategies using centrifugation for cost effective separation of microalgae cells for biodiesel applications. Bioresource Technology, 128: 241–245

    CAS  Google Scholar 

  • Farooq W, Lee Y C, Ryu B G, Kim B H, Kim H S, Choi Y E, Yang J W (2013). Two-stage cultivation of two Chlorella sp. strains by simultaneous treatment of brewery wastewater and maximizing lipid productivity. Bioresource Technology, 132: 230–238

    CAS  Google Scholar 

  • Fon Sing S, Isdepsky A, Borowitzka M A, Lewis D M (2014). Pilotscale continuous recycling of growth medium for the mass culture of a halotolerant Tetraselmis sp. in raceway ponds under increasing salinity: A novel protocol for commercial microalgal biomass production. Bioresource Technology, 161: 47–54

    CAS  Google Scholar 

  • Fret J, Roef L, Blust R, Diels L, Tavernier S, Vyverman W, Michiels M (2017). Reuse of rejuvenated media during laboratory and pilot scale cultivation of Nannochloropsis sp. Algal Research, 27: 265–273

    Google Scholar 

  • GAQSIQ(General Administration of Quality Supervision, Inspection and Quarantine of P.R. China) (2005). Standards for Irrigation Water Quality (in Chinese)

  • Gerardo M L, Oatley-Radcliffe D L, Lovitt R W (2014). Minimizing the energy requirement of dewatering scenedesmus sp. by microfiltration: Performance, costs, and feasibility. Environmental Science & Technology, 48(1): 845–853

    CAS  Google Scholar 

  • Gudin C, Thepenier C (1986). Bioconversion of solar energy into organic chemicals by microalgae. Advances in biotechnological processes, Alan R. Liss, USA, (6): 73–110

    CAS  Google Scholar 

  • Hadj-Romdhane F, Jaouen P, Pruvost J, Grizeau D, Van Vooren G, Bourseau P (2012). Development and validation of a minimal growth medium for recycling Chlorella vulgaris culture. Bioresource Technology, 123: 366–374

    CAS  Google Scholar 

  • Hadj-Romdhane F, Zheng X, Jaouen P, Pruvost J, Grizeau D, Croué J P, Bourseau P (2013). The culture of Chlorella vulgaris in a recycled supernatant: Effects on biomass production and medium quality. Bioresource Technology, 132: 285–292

    CAS  Google Scholar 

  • Huang C, Chen X, Liu T, Yang Z, Xiao Y, Zeng G, Sun X (2012). Harvesting of Chlorella sp. using hollow fiber ultrafiltration. Environmental Science and Pollution Research International, 19(5): 1416–1421

    CAS  Google Scholar 

  • Huang Y, Sun Y, Liao Q, Fu Q, Xia A, Zhu X (2016). Improvement on light penetrability and microalgae biomass production by periodically pre-harvesting Chlorella vulgaris cells with culture medium recycling. Bioresource Technology, 216: 669–676

    CAS  Google Scholar 

  • Hung M T, Liu J C (2006). Microfiltration for separation of green algae from water. Colloids and Surfaces. B, Biointerfaces, 51(2): 157–164

    CAS  Google Scholar 

  • Hwang T, Park S J, Oh Y K, Rashid N, Han J I (2013). Harvesting of Chlorella sp. KR-1 using a cross-flow membrane filtration system equipped with an anti-fouling membrane. Bioresource Technology, 139: 379–382

    CAS  Google Scholar 

  • Kim D G, La H J, Ahn C Y, Park Y H, Oh H M (2011). Harvest of Scenedesmus sp. with bioflocculant and reuse of culture medium for subsequent high-density cultures. Bioresource Technology, 102(3): 3163–3168

    CAS  Google Scholar 

  • Konig A, Pearson H W, Silva S A (1987). Ammonia toxicity to algal growth in waste stabilization ponds. Water Science and Technology, 19(12): 115–122

    CAS  Google Scholar 

  • Kovalcik D J (2013). Algal Harvesting for Biodiesel Production: Comparing Centrifugation and Electrocoagulation. Dissertation for the Doctoral Degree. College Station: Texas A&M University.

    Google Scholar 

  • Lee Y K (2004). Algal Nutrition‒Heterotrophic Carbon Nutrition. Handbook of Microalgal Culture: Biotechnology and Applied Phycology, 116–124

    Google Scholar 

  • Loftus S E, Johnson Z I (2017). Cross-study analysis of factors affecting algae cultivation in recycled medium for biofuel production. Algal Research, 24: 154–166

    Google Scholar 

  • Mackay D, Salusbury T (1988). Choosing between centrifugation and crossflow microfiltration. Chemical Engineering (Albany, N.Y.), 477: 45–50

    Google Scholar 

  • Milledge J J, Heaven S (2013). A review of the harvesting of micro-algae for biofuel production. Reviews in Environmental Science and Biotechnology, 12(2): 165–178

    Google Scholar 

  • Mo W, Soh L, Werber J R, Elimelech M, Zimmerman J B (2015). Application of membrane dewatering for algal biofuel. Algal Research, 11: 1–12

    Google Scholar 

  • Monte J, Sá M, Galinha C F, Costa L, Hoekstra H, Brazinha C, Crespo J G (2018). Harvesting of Dunaliella salina by membrane filtration at pilot scale. Separation and Purification Technology, 190: 252–260

    CAS  Google Scholar 

  • Norsker N H, Barbosa M J, Vermuë M H, Wijffels R H (2011). Microalgal production–A close look at the economics. Biotechnology Advances, 29(1): 24–27

    CAS  Google Scholar 

  • Park J B K, Craggs R J, Shilton A N (2013). Investigating why recycling gravity harvested algae increases harvestability and productivity in high rate algal ponds. Water Research, 47(14): 4904–4917

    CAS  Google Scholar 

  • Petrusevski B, Bolier G, Van Breemen A N, Alaerts G J (1995). Tangential flow filtration: a method to concentrate freshwater algae. Water Research, 29(5): 1419–1424

    CAS  Google Scholar 

  • Pires J C M, Alvim-Ferraz M C M, Martins F G, Simões M (2012). Carbon dioxide capture from flue gases using microalgae: engineering aspects and biorefinery concept. Renewable & Sustainable Energy Reviews, 16(5): 3043–3053

    CAS  Google Scholar 

  • Richardson J W, Johnson M D, Lacey R, Oyler J, Capareda S (2014). Harvesting and extraction technology contributions to algae biofuels economic viability. Algal Research, 5: 70–78

    Google Scholar 

  • Rippka R, Deruelles J, Waterbury J B, Herdman M, Stanier R Y (1979). Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology, 111(1): 1–61

    Google Scholar 

  • Rossignol N, Vandanjon L, Jaouen P, Quemeneur F (1999). Membrane technology for the continuous separation microalgae/culture medium: Compared performances of cross-flow microfiltration and ultrafiltration. Aquacultural Engineering, 20(3): 191–208

    Google Scholar 

  • SEPA (State Environmental Protection Administration of China) (2002). Methods for Monitoring and Analysis of Water and Wastewater (4th ed). Beijing: China Environmental Science Press (in Chinese)

    Google Scholar 

  • Sun X, Wang C, Tong Y, Wang W, Wei J (2013). A comparative study of microfiltration and ultrafiltration for algae harvesting. Algal Research, 2(4): 437–444

    Google Scholar 

  • Udom I, Zaribaf B H, Halfhide T, Gillie B, Dalrymple O, Zhang Q, Ergas S J (2013). Harvesting microalgae grown on wastewater. Bioresource Technology, 139: 101–106

    CAS  Google Scholar 

  • Wang T, Yabar H, Higano Y (2013). Perspective assessment of algae-based biofuel production using recycled nutrient sources: the case of Japan. Bioresource Technology, 128: 688–696

    CAS  Google Scholar 

  • Wang X F, Lu H F, Zhang L, Wang M Z, Zhao Y, Li B M (2015). Combination of Electrolysis and Microalgae cultivation to treat effluent from anaerobic digestion of poultry manure. In: Proceedings of the International Symposium on Animal Environment and Welfare 2015, Chongqing. Beijing: China Agriculture Press, 179–186

    Google Scholar 

  • Watanabe Y, Hall D O (1995). Photosynthetic CO2 fixation technologies using a helical tubular bioreactor incorporating the filamentous cyanobacterium Spirulina platensis. Energy Conversion and Management, 36(6): 721–724

    CAS  Google Scholar 

  • Xia A, Murphy J D (2016). Microalgal cultivation in treating liquid digestate from biogas systems. Trends in Biotechnology, 34(4): 264–275

    CAS  Google Scholar 

  • Yang J, Xu M, Zhang X, Hu Q, Sommerfeld M, Chen Y (2011). Lifecycle analysis on biodiesel production from microalgae: Water footprint and nutrients balance. Bioresource Technology, 102(1): 159–165

    CAS  Google Scholar 

  • Yuan X, Kumar A, Sahu A K, Ergas S J (2011). Impact of ammonia concentration on Spirulina platensis growth in an airlift photobioreactor. Bioresource Technology, 102(3): 3234–3239

    CAS  Google Scholar 

  • Zhang X, Hu Q, Sommerfeld M, Puruhito E, Chen Y (2010). Harvesting algal biomass for biofuels using ultrafiltration membranes. Bioresource Technology, 101(14): 5297–5304

    CAS  Google Scholar 

  • Zhu L (2015). Microalgal culture strategies for biofuel production: A review. Biofuels, Bioproducts & Biorefining, 9(6): 801–814

    CAS  Google Scholar 

  • Zhu L D, Takala J, Hiltunen E, Wang Z M (2013). Recycling harvest water to cultivate Chlorella zofingiensis under nutrient limitation for biodiesel production. Bioresource Technology, 144: 14–20

    CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the Program of Outstanding Talents and Innovative Research Teams in Agriculture (2011-049), China Agricultural Research System (CARS-40) and National Natural Science Foundation of China (51576206). The authors thank the technical and financial support from Minhe Animal Husbandry Incorporated Company. Thanks to Dr. Jiqin Ni (Purdue University) and Dr. Maung Thein Myint (New Mexico State University) for improving the paper. Xinfeng Wang also thanks the China Scholarship Council for financial support of visiting scholar studies in the USA.

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Authors and Affiliations

  1. Laboratory of Environment-Enhancing Energy (E2E), Key Laboratory of Agricultural Engineering in Structure and Environment, Ministry of Agriculture, College of Water Resources and Civil Engineering, China Agricultural University, Beijing, 100083, China

    Xinfeng Wang, Haifeng Lu, Zhidan Liu, Na Duan & Baoming Li

  2. Beijing Engineering Research Center for Animal Healthy Environment, Beijing, 100083, China

    Xinfeng Wang & Baoming Li

  3. Department of Civil Engineering, New Mexico State University, Las Cruces, NM, 88003, USA

    Xinfeng Wang, Lu Lin & Pei Xu

  4. Shandong Minhe Biotechnology Limited Company, Yantai, 265600, China

    Taili Dong

  5. School of Engineering, Cardiff University, Queen’s Building, Cardiff, CF24 3AA, UK

    Hua Xiao

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  1. Xinfeng Wang
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  2. Lu Lin
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Correspondence to Haifeng Lu or Baoming Li.

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Wang, X., Lin, L., Lu, H. et al. Microalgae cultivation and culture medium recycling by a two-stage cultivation system. Front. Environ. Sci. Eng. 12, 14 (2018). https://doi.org/10.1007/s11783-018-1078-z

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  • DOI: https://doi.org/10.1007/s11783-018-1078-z

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