Abstract
The synthesis of 2-butenal, which is a vital raw material for the production of sorbic acid as a food preservative, generates some toxic by-products, so it is urgent to seek better detoxification strategies for the treatment of 2-butenal manufacture wastewater. In this study, batch experiments were carried out to investigate the inhibition effect of wastewater on the methanogenic activity. To understand the wastewater toxicity to anaerobic granular sludge, variations of the specific methanogenic activity (SMA) and extracellular polymeric substance (EPS) constituents at various wastewater CODs were investigated. Ultraviolet-visible (UV-vis) spectra and Fourier transform infrared (FT-IR) spectra were employed to analyze the structure of the EPS. The results showed that the inhibitory ratio of 2-butenal manufacture wastewater was less than 8.4% on the anaerobic granular sludge when the CODs were less than 959 mg/L. However, the inhibitory ratio increased from 36.4% to 93.6% when CODs increased from 2396 mg/L to 9585 mg/L, with the SMA decreasing from 39.1 mL CH4/(gVSS∙d) to 3.2 mL CH4/(gVSS∙d). The diversity of the microbial community under various CODs was researched by Illumina 16S rRNA Miseq sequencing and the results demonstrated that Proteiniphilum, Petrimonas and Syntrophobacter were the dominant bacteria genera in all sample. Regarding archaea, Methanobacterium was the most dominated archaea genera, followed by the Methanosaeta group in all samples. Moreover, the bacterial communities had changed obviously with increasing CODs, which indicated high CODs played a negative impact on the richness and diversity of bacterial community in the sludge samples.
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Aquino S F, Stuckey D C (2004). Soluble microbial products formation in anaerobic chemostats in the presence of toxic compounds. Water Research, 38(2): 255–266
Castello E, Santos C G Y, Iglesias T, Paolino G, Wenzel J, Borzacconi L, Etchebehere C (2009). Feasibility of biohydrogen production from cheese whey using a UASB reactor: Links between microbial community and reactor performance. International Journal of Hydrogen Energy, 34(14): 5674–5682
Chen S Y, Dong X Z (2005). Proteiniphilum acetatigenes gen. nov., sp. nov., from a UASB reactor treating brewery wastewater. International Journal of Systematic and Evolutionary Microbiology, 55(Pt 6): 2257–2261
Cheng J Y, Kanjo Y, Suidan M T, Venosa A D (1996). Anaerobic biotransformation of 2,4-dinitrotoluene with ethanol as primary substrate: Mutual effect of the substrates on their biotransformation. Water Research, 30(2): 307–314
Díaz E, Amils R, Sanz J L (2003). Molecular ecology of anaerobic granular sludge grown at different conditions. Water Science and Technology, 48(6): 57–64
Dong F, Zhao Q B, Zhao J B, Sheng G P, Tang Y, Tong Z H, Yu H Q, Li Y Y, Harada H (2010). Monitoring the restart-up of an upflow anaerobic sludge blanket (UASB) reactor for the treatment of a soybean processing wastewater. Bioresource Technology, 101(6): 1722–1726
DuBois M, Gilles K A, Hamilton J K, Rebers P A, Smith F (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3): 350–356
Foladori P, Bruni L, Tamburini S (2014). Toxicant inhibition in activated sludge: fractionation of the physiological status of bacteria. Journal of Hazardous Materials, 280: 758–766
Grabowski A, Tindall B J, Bardin V, Blanchet D, Jeanthon C (2005). Petrimonas sulfuriphila gen. nov., sp. nov., a mesophilic fermentative bacterium isolated from a biodegraded oil reservoir. International Journal of Systematic and Evolutionary Microbiology, 55(Pt 3): 1113–1121
Gu A Z, Hedlund B P, Staley J T, Strand S E, Stensel H D (2004). Analysis and comparison of the microbial community structures of two enrichment cultures capable of reductively dechlorinating TCE and cis-DCE. Environmental Microbiology, 6(1): 45–54
Ioannou L A, Li Puma G, Fatta-Kassinos D (2015). Treatment of winery wastewater by physicochemical, biological and advanced processes: A review. Journal of Hazardous Materials, 286: 343–368
Klindworth A, Pruesse E, Schweer T, Peplies J, Quast C, Horn M, Glöckner F O (2013). Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencingbased diversity studies. Nucleic Acids Research, 41(1): e1
Krakat N, Schmidt S, Scherer P (2011). Potential impact of process parameters upon the bacterial diversity in the mesophilic anaerobic digestion of beet silage. Bioresource Technology, 102(10): 5692–5701
Li Y F, Shi J, Nelson M C, Chen P H, Graf J, Li Y B, Yu Z T (2016). Impact of different ratios of feedstock to liquid anaerobic digestion effluent on the performance and microbiome of solid-state anaerobic digesters digesting corn stover. Bioresource Technology, 200: 744–752
Lin R C, Cheng J, Ding L K, Song W L, Zhou J H, Cen K F (2015). Sodium borohydride removes aldehyde inhibitors for enhancing biohydrogen fermentation. Bioresource Technology, 197: 323–328
Liu C, Li H, Zhang Y Y, Si D D, Chen Q W (2016). Evolution of microbial community along with increasing solid concentration during high-solids anaerobic digestion of sewage sludge. Bioresource Technology, 216: 87–94
Mizuno K, Morishita Y, Ando A, Tsuchiya N, Hirata M, Tanaka K (2012). Genus-specific and phase-dependent effects of nitrate on a sulfate-reducing bacterial community as revealed by dsrB-based DGGE analyses of wastewater reactors. World Journal of Microbiology & Biotechnology, 28(2): 677–686
Morgan J W, Forster C F, Evison L (1990). A comparative study of the nature of biopolymers extracted from anaerobic and activated sludges. Water Research, 24(6): 743–750
Ohtsuki T, Sato K, Sugimoto N, Akiyama H, Kawamura Y (2012). Absolute quantitative analysis for sorbic acid in processed foods using proton nuclear magnetic resonance spectroscopy. Analytica Chimica Acta, 734: 54–61
Oliveira S VWB, Moraes E M, Adorno MA T, Varesche MB A, Foresti E, Zaiat M (2004). Formaldehyde degradation in an anaerobic packed-bed bioreactor. Water Research, 38(7): 1685–1694
Owen WF, Stuckey D C, Healy Jr J B, Young L Y, McCarty P L (1979). Bioassay for monitoring biochemical methane potential and anaerobic toxicity. Water Research, 13(6): 485–492
Pereira N S, Zaiat M (2009). Degradation of formaldehyde in anaerobic sequencing batch biofilm reactor (ASBBR). Journal of Hazardous Materials, 163(2–3): 777–782
Shakoori A R, Makhdoom M, Haq R U (2000). Hexavalent chromium reduction by a dichromate-resistant gram-positive bacterium isolated from effluents of tanneries. Applied Microbiology and Biotechnology, 53(3): 348–351
Tran T, Bolto B, Gray S, Hoang M, Ostarcevic E (2007). An autopsy study of a fouled reverse osmosis membrane element used in a brackish water treatment plant. Water Research, 41(17): 3915–3923
Venkiteshwaran K, Milferstedt K, Hamelin J, Fujimoto M, Johnson M, Zitomer D H (2017). Correlating methane production to microbiota in anaerobic digesters fed synthetic wastewater. Water Research, 110: 161–169
Wang Y, Wang Q H, Li M, Yang Y N, He W, Yan G X, Guo S H (2016). An alternative anaerobic treatment process for treatment of heavy oil refinery wastewater containing polar organics. Biochemical Engineering Journal, 105: 44–51
Wang Z C, Gao M C, Wang S, Xin Y J, Ma D, She Z L, Wang Z, Chang Q B, Ren Y (2014). Effect of hexavalent chromium on extracellular polymeric substances of granular sludge from an aerobic granular sequencing batch reactor. Chemical Engineering Journal, 251: 165–174
Wang Z W, Wu Z C, Tang S J (2009). Extracellular polymeric substances (EPS) properties and their effects on membrane fouling in a submerged membrane bioreactor. Water Research, 43(9): 2504–2512
Wei D, Li MT, Wang X D, Han F, Li L S, Guo J, Ai L J, Fang L L, Liu L, Du B, Wei Q (2016). Extracellular polymeric substances for Zn (II) binding during its sorption process onto aerobic granular sludge. Journal of Hazardous Materials, 301: 407–415
Xie S B, Wu Y Q, Wang WT, Wang J S, Luo Z P, Li S Y (2014). Effects of acid/alkaline pretreatment and gamma-ray irradiation on extracellular polymeric substances from sewage sludge. Radiation Physics and Chemistry, 97: 349–353
Zhang D Q, Tan S K, Gersberg R M (2010). Municipal solid waste management in China: status, problems and challenges. Journal of Environmental Management, 91(8): 1623–1633
Zhang X W, Yue Q Y, Yue D T, Gao B Y, Wang X J (2015). Application of Fe0/C/Clay ceramics for decoloration of synthetic Acid Red 73 and Reactive Blue 4 wastewater by micro-electrolysis. Frontiers of Environmental Science & Engineering, 9(3): 402–410
Zhao X G, Jiang G W, Li A, Li Y (2016). Technology, cost, a performance of waste-to-energy incineration industry in China. Renewable & Sustainable Energy Reviews, 55: 115–130
Zhu L, Qi H Y, Lv M L, Kong Y, Yu Y W, Xu X Y (2012). Component analysis of extracellular polymeric substances (EPS) during aerobic sludge granulation using FTIR and 3D-EEM technologies. Bioresource Technology, 124: 455–459
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We offer our appreciation to project supported by the China Special S&T Project on Treatment and Control of Water Pollution (2017ZX07402-002).
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Song, G., Xi, H., Sun, X. et al. Effect of 2-butenal manufacture wastewater to methanogenic activity and microbial community. Front. Environ. Sci. Eng. 12, 10 (2018). https://doi.org/10.1007/s11783-018-1056-5
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DOI: https://doi.org/10.1007/s11783-018-1056-5