Detection of oxidative stress and DNA damage in freshwater snail Lymnea leuteola exposed to profenofos
- 27 Downloads
Extensive production and use of organophosphate pesticide in agriculture, has risen concerned about its ecotoxicity and risk assessment of insecticides, which are more important. Therefore, the present investigation was aimed to study the induction of oxidative stress and DNA damage by organophosphate insecticide profenofos (PFF) in freshwater snail Lymnea luteola (L. luteola). The median lethal value (96 h LC50) of PFF was estimated as 1.26 mg/L for L. luteola in a semi-static system and on the basis of LC50 value three concentrations viz., 0.126 (1/10 of LC50, Sublethal I), 0.63 (1/2 of LC50, Sublethal II) and 0.84 mg/L (2/3 of LC50, Sublethal III) were determined. Snails were exposed to above-mentioned concentrations of PFF along with solvent control (acetone) and negative control for 96 h. The haemolymph was collected at 24 and 96 h of after treatment. In heamolymph of PFF exposed snail, lipid peroxide, glutathione reduced glutathione S transferase and superoxide dismutase activities at the tested concentrations significantly differ from those in the control. The genotoxicity induced in hemocytes of treated snails was measured by alkaline single cell gel electrophoresis assay. The data of this experiment demonstrated significantly enhancement of oxidative stress and DNA damage in the treated snails as compared to controls. Also, we observed statistically significant correlations of ROS with DNA damage (% tail DNA) (R2 = 0.9708) for 24 h and DNA damage (R2 = 0.9665) for 96 h.
KeywordsAcute toxicity Profenofos ROS oxidative stress DNA damage Lymnea luteola
The authors are grateful to the Deanship of Scientific Research at King Saud University for funding this research (RG-1435-076).
- Ali D, Nagpure N S, Kumar S, Kumar R, Kushwaha B, Lakra W S (2009). Assessment of genotoxic and mutagenic effects of chlorpyrifos in freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Food and Chemical Toxicology, 47(3): 650–656CrossRefGoogle Scholar
- APHA AWWA, WPCF (1998). Standard methods for examination of water and wastewater, 20th ed. American Public Health Association, New YorkGoogle Scholar
- Becker J S, Füllner K, Seeling U D, Fornalczyk G, Kuhn A J (2008). Measuring magnesium, calcium and potassium isotope ratios using ICP-QMS with an octopole collision cell in tracer studies of nutrient uptake and translocation in plants. Analytical Bioanalytical Chemistry, 390(2): 571–578CrossRefGoogle Scholar
- Finney D J (1971). Probit Analysis. Cambridge: Cambridge University Press, 333 pGoogle Scholar
- Malla T M, Senthikumar C S, Akhtar S, Ganesh N (2011). Micronuclei as an evidence of DNA damage in fresh water catfish Heteroneustes fossilis (Blotch) exposed to synthetic sindoor, ARPN Journal of Agricultural and Biological Science, 95: 351–358Google Scholar
- Miller G T (2004). Chapter 9, Sustaining the Earth, 6th ed. Pacific Grove, California: Thompson Learning, Inc., 211–216Google Scholar
- Monteiro D A, de Almeida J A, Rantin F T, Kalinin A L (2006). Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology—Part C, 143(2): 141–149Google Scholar
- Palmer W E, Bromley P T, Brandenburg R L (2007). Wildlife and pesticides-peanuts. North Carolina Cooperative Extension ServiceGoogle Scholar
- Rank J, Jensen K, Jespersen PH (2005). Monitoring DNA damage in indigenous blue mussels (Mytilus edulis) sampled from coastal sites in Denmark Mutation Research, 585: 33–42Google Scholar
- Yassi A, Kjellstrom T (1997). Linkages between environmental and occupational health. Chapter 53 environmental health hazards PartVII-The environment. Encyclopaedia of occupational health and safety, 4th ed. www.ilocis.org/documents/chpt53e.htmGoogle Scholar