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More severe toxicity of gold nanoparticles with rougher surface in mouse hippocampal neurons

金纳米颗粒表面越粗糙对小鼠海马神经元的毒性越强

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Abstract

Gold nanoparticles (GNPs) have been extensively used in nanomedicine and neuroscience owing to their biological inertness, peculiar opto-electronic and physico-chemical features. However, the effect of GNPs shape on the neurophysiological properties of single neuron is still unclear. To tackle this issue, different shape GNPs (nanosphere, nanotriakisoctahedron and nanoflower) were synthesized to investigate the effect of GNPs on the voltage-dependent sodium channel and the action potential (AP) of hippocampal CA1 neurons in mice. The results indicated that GNPs inhibited the amplitudes of voltage-gated sodium current (INa) and led to a hyperpolarizing shift in the voltage-dependence curve of both activation and inactivation of INa. GNPs also increased neuronal excitability and altered some properties of AP. Moreover, most alterations in AP properties were observed in nanoflower GNPs treated CA1 neurons, suggesting that the neurotoxicity of gold nanoparticles is surface roughness-dependent. These results may provide a valuable direction in the clinical application of GNPs.

摘要

金纳米颗粒(GNPs), 由于其具有良好的生物稳定性、独特的光电和物理化学特性, 已被广泛用 于纳米医学和神经科学。但是, 不同形貌的GNPs对神经元的神经生理特性的影响仍不清楚。为解决 此问题, 合成了不同形状的GNPs(纳米球, 纳米六面体和纳米花)以研究不同表面粗糙度的GNPs对小 鼠海马CA1 神经元电压依赖性钠通道和动作电位(AP)的影响。结果表明, GNPs抑制了电压门控钠电 流(INa)的电流幅度, 使INa激活和失活的电压依赖性激活和失活曲线向超级化方向偏移。GNPs还增加了 神经元兴奋性并改变了动作电位的幅度、阈值、半波宽等参数变化。此外, 在纳米花GNPs处理过的 CA1 神经元中观察到了强度最大的AP改变(六面体次之, 球最小), 这表明金纳米颗粒的神经毒性与表 面粗糙度呈正相关。这些结果可能为GNPs的临床应用提供有价值的参考。

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Funding

Project(LY19C090004) supported by the Natural Science Foundation of Zhejiang Province, China; Projects(BK20200710, BK2018077) supported by the Natural Science Foundation of Jiangsu Province, China; Project(NHKY-2019-19) supported by the Nanjing Polytechnic Institute Start Fund, China; Project(202012920026Y) supported by the Innovation and Entrepreneurship Training Program of Jiangsu Province College Students, China

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Authors and Affiliations

  1. School of Optometry and Ophthalmology and Eye Hospital, Wenzhou Medical University, State Key Laboratory of Optometry, Ophthalmology, and Vision Science, Wenzhou, 325027, China

    Xin Lin  (林鑫), Rong Cui  (崔蓉) & Bo Chen  (陈波)

  2. School of Physical and Mathematical Sciences, Institute of Advances Materials, Nanjing Tech University, Nanjing, 211816, China

    Yan-ling Hu  (胡艳玲) & Dong-liang Yang  (杨栋梁)

  3. Nanjing Polytechnic Institute, Nanjing, 210048, China

    Yan-ling Hu  (胡艳玲), Chi Zhang  (张驰) & Jie Yin  (殷杰)

  4. Shenzhen Key Laboratory of Drug Addiction, CAS Key Laboratory of Brain Connectome and Manipulation, the Brain Cognition and Brain Disease Institute (BCBDI), Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences; Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions, Shenzhen, 518055, China

    Bo Chen  (陈波)

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  1. Xin Lin  (林鑫)
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Lin, X., Hu, Yl., Zhang, C. et al. More severe toxicity of gold nanoparticles with rougher surface in mouse hippocampal neurons. J. Cent. South Univ. 28, 3642–3653 (2021). https://doi.org/10.1007/s11771-021-4844-1

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