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Activities of laccase produced by a strains Penicillium simplicissimum induced by chemical agentia and UV radiation

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Abstract

Penicillium simplicissimum was cultured and preserved on the potato dextrose agar (PDA) medium. PDA-RBBR (Remazal Brilliant Blue R) medium was used for the screening of the strains, which is able to produce enzymes. After the mutation process in Penicillium simplicissimum induced by chemical reagent and ultraviolet radiation, a high laccase-producing strains Penicillium simplicissimum was obtained. When 5 mL diethyl sulfate (2%) was mixed along with 5 mL spore suspension for 30 min, chemical mutagenesis reached its best condition. And the optimum conditions of UV mutagenesis were that spore suspension was irradiated for 4 min under 15 W UV lamp at a distance of 30 cm. The highest activity of C5E4 strains was 4.80 U/g over 18% higher than the maximum laccase activity of original microorganism. Five generations of the mutant strains were cultured, and the laccase activity of the strains was measured. The result showed that C5E4 strains can product laccase of the five subcultures stably.

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Authors and Affiliations

  1. Department of Biological and Environmental Engineering, Changsha University, Changsha, 410003, China

    Xue Li  (李雪), Ya Pang  (庞娅), Kun Luo  (罗琨) & Xing-sheng Liao  (廖兴盛)

  2. School of Information and Safety Engineering, Zhongnan University of Economics and Law, Wuhan, 430073, China

    Fei Li  (李飞)

  3. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, China

    Cui Lai  (赖萃) & Jin-hui Huang  (黄瑾辉)

Authors
  1. Xue Li  (李雪)
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  2. Fei Li  (李飞)
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  3. Cui Lai  (赖萃)
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  4. Jin-hui Huang  (黄瑾辉)
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  5. Ya Pang  (庞娅)
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  6. Kun Luo  (罗琨)
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  7. Xing-sheng Liao  (廖兴盛)
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Corresponding authors

Correspondence to Xue Li  (李雪) or Fei Li  (李飞).

Additional information

Foundation item: Projects(51178172, 51308076, 51408206, 51578222) supported by the National Natural Science Foundation of China; Project(113049A) supported by the Ministry of Education of China

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Li, X., Li, F., Lai, C. et al. Activities of laccase produced by a strains Penicillium simplicissimum induced by chemical agentia and UV radiation. J. Cent. South Univ. 24, 1953–1958 (2017). https://doi.org/10.1007/s11771-017-3603-9

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  • DOI: https://doi.org/10.1007/s11771-017-3603-9

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