Journal of Central South University

, Volume 23, Issue 11, pp 2784–2791 | Cite as

Synthesis of size-controllable Fe3O4 magnetic submicroparticles and its biocompatible evaluation in vitro

  • Qing-hua Tian (田庆华)
  • Wen-bo Ning (宁文博)
  • Wei-jia Wang (王惟嘉)
  • Xiu-hong Yuan (袁秀洪)
  • Zhi-ming Bai (白志明)
Materials, Metallurgy, Chemical and Environmental Engineering


Large scaled uniform and size-controllable magnetic submicroparticles (MSPs) were synthesized via solvothermal method with ferric chloride as iron source and sodium acetate as trapping agent. The influence of Fe3+ and NaAc contents on the size distribution of MSPs was investigated. The structural and morphological properties of the synthesized particles were studied by scanning electron microscopy (SEM), X-ray power diffraction (XRD) and vibrating sample magnetometer (VSM). The well-dispersed MSPs with size of 100-1000 nm were obtained by simply adjusting the contents of Fe3+ and NaAc. In addition, the hemolysis and cytotoxicity of Fe3O4 MSPs, and their ability to case arrest in cell life-cycles were studied. The results indicate that larger size could lead to lower hemolysis. From MTT(3-(4,5-dimethylthuazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the interactions between MSPs and adhesive mouse fibroblast cell line(L929) were probed. Larger size of Fe3O4 MSPs demonstrates lower cell viability following an exposure to the cells.


magnetite submicroparticles biocompatibility hemolysis cytotoxicity 


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Copyright information

© Central South University Press and Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Qing-hua Tian (田庆华)
    • 1
  • Wen-bo Ning (宁文博)
    • 1
  • Wei-jia Wang (王惟嘉)
    • 1
  • Xiu-hong Yuan (袁秀洪)
    • 1
  • Zhi-ming Bai (白志明)
    • 2
  1. 1.School of Metallurgy and EnviromentCental South UniversityChangshaChina
  2. 2.Haikou Municipal People’s HospitalHaikouChina

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