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Response of enzymatic and non-enzymatic antioxidant defense systems of Polygonum hydropiper to Mn stress

  • Materials, Metallurgy, Chemical and Environmental Engineering
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Abstract

The response of enzyme and non-enzymatic antioxidants of Mn hyperaccumuator, Polygonum hydropiper (P. hydropiper), to Mn stress was studied using hydroponics culture experiments to explore the mechanism of Mn tolerance in this species. Results showed that both chlorophyll and carotenoid contents significantly (p<0.05) decreased with increasing Mn treatment levels (0, 0.5, 1, 2, 4, and 8 mg/L) in hydroponics. The concentrations of malondialdehyde (MDA) and hydrogen peroxide (H2O2) in the root and shoot of P. hydropiper were accumulated under Mn stress. Meanwhile, the anti-oxidative functions of several important enzymes, including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and peroxidase (POD) in plants were stimulated by Mn spike in leaves and roots, especially at low Mn stress; while sulfhydryl group (—SH) and glutathion (GSH) were likely involved in Mn detoxification of P. hydropiper under high Mn stress.

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References

  1. RASKIN I, ENSLEY B D. Phytoremediation of toxic metals: Using plants to clean up the environment [M]. New York: John Wiley & Sons, Inc, 2000: 198.

    Google Scholar 

  2. SALT D E, SMITH R D, RASKIN I. Phytoremediation [J]. Annu Rev Plant Physiol: Plant Molecular Biology 1998, 49: 643–668.

    Article  Google Scholar 

  3. YANG Qing-wei, ZENG Qing, XIAO Fei, LIU Xue-lian, PAN Jin, HE Jin-feng, LI Zhi-yong. Investigation of manganese tolerance and accumulation of two Mn hyperaccumulators Phytolacca americana L. and Polygonum hydropiper L. in the real Mn-contaminated soils near a manganese mine [J]. Environmental Earth Sciences 2013, 68: 1127–1134.

    Article  Google Scholar 

  4. ZHOU Jian-hua, YANG Qing-wei, LAN Chong-yu, YE Zhi-hong. Heavy metal uptake and extraction potential of two Bechmeria nivea (L.) Gaud. (Ramie) varieties associated with chemical reagents [J]. Water Air and Soil Pollution 2010, 211: 359–366.

    Article  Google Scholar 

  5. ZHUANG Ping, YANG Qin-wen, WANG Hong-bin, SHU Wen-sheng. Phytoextraction of heavy metals by eight plant species in the field [J]. Water Air and Soil Pollution 2007, 184(1): 235–242.

    Article  Google Scholar 

  6. YU Fang-ming, LIU Ke-hui, ZHOU Zhen-ming, DENG Hua, CHEN Bin. Effects of cadmium on enzymatic and non-enzymatic antioxidative defenses of rice (Oryza saliva L.)[J]. International Journal of Phytoremediation 2013, 15: 513–521.

    Article  Google Scholar 

  7. LIU Jie, SHANG Wei-wie, ZHANG Xue-hong, ZHU Yin-ian, YU Ke. Mn accumulation and tolerance in Celosia argentea Linn: A new Mn-hyperaccumulating plant species [J]. Journal of Hazardous Materials 2014, 267: 136–141.

    Article  Google Scholar 

  8. STOCHS S J, BAGCHI D. Oxidative mechanism in the toxicity of metal ions [J]. Free Radical Biology and Medicine 1995, 18: 321–336.

    Article  Google Scholar 

  9. DIXIT V, PANDEY V, SHYAM R. Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad) [J]. Journal of Experiment Botany 2001, 52: 1101–1109.

    Article  Google Scholar 

  10. MITTLER R. Oxidative stress, antioxidants and stress tolerance [J]. Trends in Plant Science 2002, 7: 405–410.

    Article  Google Scholar 

  11. WÓJCIK M, SKÓRZYNSKA-POLIT E, TUKIENDORF A. Organic acids accumulation and antioxidant enzyme activities in Thlaspi caerulescens under Zn and Cd stress [J]. Plant Growth Regulation 2006, 48: 145–155.

    Article  Google Scholar 

  12. WANG Zi, ZHANG Yu-xiu, HUANG Zi-bo, HUANG Lin. Antioxidative response of metal-accumulator and non-accumulator plants under cadmium stress [J]. Plant Soil 2008, 310: 137–149.

    Article  Google Scholar 

  13. WANG Hua, TANG Shu-mei, LIAO Xiang-jun, CAO Qi-min, YANG An-fu, WANG Ting-zhong. A new manganese-hyperaccumulator: Polygonum hydropiper L [J]. Ecology and Environment 2007, 16: 830–834. (in Chinese)

    Google Scholar 

  14. VELIKOVA V, YORDANOV I, EDREVA A. Oxidative stress and some antioxidant system in acid rain treated bean plants: Protective role of exogenous polyamines [J]. Plant Science 2000, 151: 59–66.

    Article  Google Scholar 

  15. SNELLER F E C, van HEERWAARDEN L M, KOEVOETS P L M, VOOIJS R, SCHAT H, VERKLEIJ J A C. Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmium: Comparison of derivatization with Ellman’s reagent and monobromobimane [J]. Journal of Agricultural and Food Chemistry 2000, 48: 4014–4019.

    Article  Google Scholar 

  16. BAKER A J M, BROOKS R R. Terrestrial higher plants which hyperaccumulate metallic elements—A review of their distribution, ecology and phytochemistry [J]. Biorecover 1989, 1: 81–126.

    Google Scholar 

  17. GALLEGO S M, BENAVÍDES M P, TOMARO M L. Effect of heavy metal ion excess on sunflower leaves: Evidence for involvement of oxidative stress [J]. Plant Science 1996, 121: 151–159.

    Article  Google Scholar 

  18. HALLIWELL B, GUTTERIDGE J M C. Free radicals in biology and medicine [M]. 2nd ed. Oxford: Clarendon Press, 2001: 136.

    Google Scholar 

  19. SHI Qing-hua, ZHU Zhu-jun. Effects of exogenous salicylic acid on manganese toxicity, element contents and antioxidative system in cucumber [J]. Environment Experiment Botany 2008, 63: 317–326.

    Article  Google Scholar 

  20. TIRYAKIOGLU M, EKER S, OZKUTKU F, HUSTED S I. Cakmak Antioxidant defence system and cadmium uptake in barley genotypes differing in cadmium tolerance [J]. Journal of Trace Elements in Medicine and Biology 2006, 20: 181–189.

    Article  Google Scholar 

  21. SANDALIO L M, DALURZO H C, GÓMEZ M, ROMERO-PUERTAS M C, DEL RÍO L A. Cadmium-induced changes in the growth and oxidative metabolism of pea plants [J]. Journal of Experimental Botany 2001, 52: 2115–2126.

    Google Scholar 

  22. MISHRA S, SRIVASTAVA S, TRIPATHI R D, GOVINDARAJAN R, KURIAKOSE S V, PRASAD M N V. Phytochelatin synthesis and response of antioxidants during cadmium stress in Bacopa monnieri L [J]. Plant Physiology and Biochemistry 2006, 44: 25–37.

    Article  Google Scholar 

  23. EBBS S, AHNER L I B, KOCHIAN L. Phytochelatin synthesis is not responsible for Cd tolerance in the Zn/Cd hyperaccumulator Thlaspi caerulescenes (J. and C. Presl)[J]. Planta 2002, 214: 635–640.

    Article  Google Scholar 

  24. SETH C S, CHATURVEDI P K, MISRA V. The role of phytochelatins and antioxidants in tolerance to Cd accumulation in Brassica juncea L [J]. Ecotoxicology and Environmental Safety 2008, 71: 76–85.

    Article  Google Scholar 

  25. XIANG C B, WERNER B L, CHRISTENSEN E M, OLIVER D J. The biological functions of glutathione revisited in Arabidopsis transgenic plants with altered glutathione levels [J]. Plant Physiology 2001, 126: 564–574.

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of City Construction, Shaoyang University, Shaoyang, 422004, China

    Xian-jun Yang  (杨贤均)

  2. College of Resource and Environment, Guangxi Normal University, Guilin, 541004, China

    Dong-mei Deng  (邓冬梅) & Fang-ming Yu  (于方明)

  3. College of Life and Environmental Science, Guilin University of Electronic Technology, Guilin, 541004, China

    Ke-hui Liu  (刘可慧)

Authors
  1. Xian-jun Yang  (杨贤均)
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  2. Dong-mei Deng  (邓冬梅)
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  3. Ke-hui Liu  (刘可慧)
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  4. Fang-ming Yu  (于方明)
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Corresponding author

Correspondence to Fang-ming Yu  (于方明).

Additional information

Foundation item: Project(41161057) supported by the National Natural Science Foundation of China; Project(Guikezhuan 14122008-2) supported by Guangxi Provincial Science and Technology Development, China; Project(2014GXNSFAA118303) supported by the Natural Science Foundation of Guangxi Province, China; Projects(YRHJ15K002, YRHJ15Z026) supported by Key Laboratory of Karst Ecology and Environment Change of Guangxi Normal University, China; Project(2016JJ6135) supported by the Natural Science Foundation of Hunan Province, China

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Yang, Xj., Deng, Dm., Liu, Kh. et al. Response of enzymatic and non-enzymatic antioxidant defense systems of Polygonum hydropiper to Mn stress. J. Cent. South Univ. 23, 793–797 (2016). https://doi.org/10.1007/s11771-016-3125-x

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  • DOI: https://doi.org/10.1007/s11771-016-3125-x

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