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Electrochimical determination of uric acid, xanthine and hypoxanthine by poly(xylitol) modified glassy carbon electrode

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Abstract

The novel covalently modified glassy carbon electrode with poly(xylitol) was prepared using an electropolymerization technique for the simultaneous determination of uric acid (UA), xanthine (XA) and hypoxanthine (HX). This new electrode presents an excellent electrocatalytic activity towards the oxidation of UA, XA and HX by cyclic voltammetry (CV) method. The oxidation peaks of the three compounds were well defined and had enhanced the peak currents. The separation potentials of the oxidation peak potentials for UA-XA and XA-HX were 380 and 370 mV in CV, respectively. Using differential pulse voltammetry (DPV) method, the calibration curves in the ranges of 5–55, 1.3–75.3 and 4–59 μmol/L were obtained for HX, XA and UA, respectively. The lowest detection limits (S/N=3) were 4.5, 0.75 and 3.75 μmol/L for HX, XA and UA, respectively. The practical application of the modified electrode was demonstrated by the determination of UA, XA, HX in human urine samples.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Engineering, Changchun University of Science and Technology, Changchun, 130022, China

    Zhi-yu Dou  (窦志宇), Li-li Cui  (崔丽莉) & Xing-quan He  (何兴权)

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  1. Zhi-yu Dou  (窦志宇)
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  2. Li-li Cui  (崔丽莉)
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  3. Xing-quan He  (何兴权)
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Correspondence to Xing-quan He  (何兴权).

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Foundation item: Project(201215135) supported by the Natural Science Foundation of Jilin Province, China

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Dou, Zy., Cui, Ll. & He, Xq. Electrochimical determination of uric acid, xanthine and hypoxanthine by poly(xylitol) modified glassy carbon electrode. J. Cent. South Univ. 21, 870–876 (2014). https://doi.org/10.1007/s11771-014-2012-6

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  • DOI: https://doi.org/10.1007/s11771-014-2012-6

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