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Influence of copper solvent extractant on microbial community structure of acidophilic microorganisms

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Abstract

The influence of different concentrations of copper solvent extractant ZJ 988 on the growth and activity of acidophilic microorganisms was studied and the microbial community structures were compared by 16S rRNA gene clone library analysis. The total bacteria numbers are reduced when 0.5% (volume fraction) extractant is added. The proportions of Acidithiobacillus ferrooxidans and Acidiphilium organovorum are increased, whereas the proportion of Leptospirillum ferriphilum is reduced. When the concentration of extractant is elevated to 1%, growth of all bacteria is inhibited. Clone library results reveal that the dominant bacteria in the culture solution with/without the extractant are At. ferrooxidans, A. organovorum and L. ferriphilum. The sensitivity order of the three bacteria to the extractant from the most to the least is found to be L. ferriphilum>At. ferrooxidans>A. organovorum.

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Authors and Affiliations

  1. National Engineering Laboratory of Biohydrometallurgy, General Research Institute for Nonferrous Metals, Beijing, 100088, China

    Bo-wei Chen  (陈勃伟), Wen-juan Li  (李文娟), Xing-yu Liu  (刘兴宇), Gui-ying Zhou  (周桂英) & Jian-kang Wen  (温建康)

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  1. Bo-wei Chen  (陈勃伟)
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  2. Wen-juan Li  (李文娟)
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  3. Xing-yu Liu  (刘兴宇)
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  4. Gui-ying Zhou  (周桂英)
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  5. Jian-kang Wen  (温建康)
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Correspondence to Jian-kang Wen  (温建康).

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Foundation item: Project(50904011) supported by the National Natural Science Foundation of China

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Chen, Bw., Li, Wj., Liu, Xy. et al. Influence of copper solvent extractant on microbial community structure of acidophilic microorganisms. J. Cent. South Univ. Technol. 17, 1196–1200 (2010). https://doi.org/10.1007/s11771-010-0618-x

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  • DOI: https://doi.org/10.1007/s11771-010-0618-x

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