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Acidophilic bacterial community reflecting pollution level of sulphide mine impacted by acid mine drainage

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Abstract

To reveal the impact of mining on bacterial ecology around mining area, bacterial community and geochemical characteristics about Dabaoshan Mine (Guangdong Province, China) were studied. By amplified ribosomal DNA restriction analysis and phylogenetic analysis, it is found that mining pollution greatly impacts the bacterial ecology and makes the habitat type of polluted environments close to acid mine drainage (AMD) ecology. The polluted environment is acidified so greatly that neutrophil and alkaliphilic microbes are massively dead and decomposed. It provided organic matters that can make Acidiphilium sp. rapidly grow and become the most bacterial species in this niche. Furthermore, Acidithiobacillus ferrooxidans and Leptospirillum sp. are also present in this niche. The amount of Leptospirillum sp. is far more than that of Acidithiobacillus ferrooxidans, which indicates that the concentration of toxic ions is very high. The conclusions of biogeochemical analysis and microbiological monitor are identical. Moreover, because the growth of Acidithiobacillus ferrooxidans and Leptospirillum sp. depends on ferrous iron or inorganic redox sulfur compounds which can be supplied by continual AMD, their presence indicates that AMD still flows into the site. And the area is closer to the outfalls of AMD, their biomasses would be more. So the distinction of their biomasses among different areas can help us to find the effluent route of AMD.

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Authors and Affiliations

  1. School of Resources Processing and Bioengineering, Central South University, Changsha, 410083, China

    Min-xi Wan  (万民熙), Yu Yang  (杨 宇), Guan-zhou Qiu  (邱冠周), Ai-ling Xu  (徐爱玲), Lin Qian  (钱 林), Zhi-ying Huang  (黄芝英) & Jin-lan Xia  (夏金兰)

  2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha, 410083, China

    Yu Yang  (杨 宇), Guan-zhou Qiu  (邱冠周) & Jin-lan Xia  (夏金兰)

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  1. Min-xi Wan  (万民熙)
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  2. Yu Yang  (杨 宇)
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  3. Guan-zhou Qiu  (邱冠周)
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  4. Ai-ling Xu  (徐爱玲)
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  5. Lin Qian  (钱 林)
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  6. Zhi-ying Huang  (黄芝英)
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  7. Jin-lan Xia  (夏金兰)
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Corresponding author

Correspondence to Guan-zhou Qiu  (邱冠周).

Additional information

Foundation item: Project(50621063) supported by the Science Fund for Creative Research Groups of China; Project(2004CB619201) supported by the Major State Basic Research Development Program of China

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Wan, Mx., Yang, Y., Qiu, Gz. et al. Acidophilic bacterial community reflecting pollution level of sulphide mine impacted by acid mine drainage. J. Cent. South Univ. Technol. 16, 223–229 (2009). https://doi.org/10.1007/s11771-009-0038-y

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  • DOI: https://doi.org/10.1007/s11771-009-0038-y

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