Skip to main content
Log in

Bioleaching of sphalerite by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans cultured in 9K medium modified with pyrrhotite

  • Published:
Journal of Central South University of Technology Aims and scope Submit manuscript

Abstract

Elective culture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans in 9K medium modified with pyrrhotite was studied. Bioleaching of flotation concentrate of sphalerite by the selected bacteria was carried out. The results show that the microorganisms cultured by pyrrhotite are a mixture of Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans, of which the capability to oxidize ferrous to ferric irons is enhanced by the high mass ratio of Fe to S in pyrrhotite. Three pyrrhotite samples were separated into various parts with corresponding S/Fe ratios by magnetic separation and were used to culture the elective bacteria as the substrate. The association of the cultures could provide a more rapid and complete oxidation of sphalerite than that of bacteria cultivated by conventional methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. MILLER P C, RHODES M K, WINBY R. Commercialization of bioleaching for base-metal extraction [J]. Minerals & Metallurgical Processing, 1999, 16(4): 42–50.

    Google Scholar 

  2. EHRLICH H L. Past, present and future of bioleaching [J]. Hydrometallurgy, 2001, 59(2/3): 127–134.

    Article  Google Scholar 

  3. BOON M, BRASSER H J, HANSFORD G. Comparison of the oxidation kinetics of different pyrite in the presence of Thiobacillus ferrooxidans or Leptospirillum ferrooxidans [J]. Hydrometallurgy, 1999, 53(1): 57–72.

    Article  Google Scholar 

  4. SAND W, RHODE K, SOBOTKE B. Evaluation of Leptospirillum ferrooxidants for leaching [J]. Appl Environ Microbiol, 1992, 58(1): 85–92.

    Google Scholar 

  5. LUO Lian-ming, WANG Jun, XU Jing. Study on methods of improving bacterial leaching velocity of gold ores [J]. Conservation and Utilization of Mineral Resources, 1994(4): 40–43. (in Chinese)

  6. GARCIA J O, MIGHAM B J, TUOVIMEN O. Sphalerite oxidation by Thiobacillus ferrooxidans and thiobacillus thiooxidans [J]. Can J Microbiol, 1995, 41: 578–584.

    Article  Google Scholar 

  7. KONISHI Y, KUBO H, ASIA S. Bioleaching of zinc sulfide concentration by Thiobacillus ferrooxidants [J]. Biotechnol Bioeng, 1992, 39: 66–74.

    Article  Google Scholar 

  8. CHOI W K, TORMA A E, OHILINE R. Electrochemical aspects of zinc sulphide leaching by Thiobacillus ferrooxidans [J]. Hydrometallurgy, 1993, 33(1/2): 137–152.

    Article  Google Scholar 

  9. LAN Zhuo-yue, HU Yue-hua, LIU Jian-she, WANG Jun. Solvent extraction of copper and zinc from bioleaching solutions with LIX984 and D2E [J]. Journal of Central South University of Technology, 2005, 12(1): 45–49.

    Article  Google Scholar 

  10. QIN Wen-qing, LAN Zhuo-yue, LI Wei-zhong. Recovery of zinc from low-grade zinc oxide ores by solvent extraction [J]. Journal of Central South University of Technology, 2003, 10(2): 98–102.

    Article  Google Scholar 

  11. LIANG Dong-yun, HE Guo-wei, ZOU Ni. The isomeromorphism of pyrrhotite and its treatment feature differentia [J]. Journal of Guangdong Nonferrous Metals, 1997, 7(1): 1–5. (in Chinese)

    Google Scholar 

  12. XIA Xue-hui. A kind of pyrrhotite from pyrite deposits in east Liaoning rift mineralogical implications [J]. Geology of Chemical Minerals, 1996, 18(4): 263–270. (in Chinese)

    MathSciNet  Google Scholar 

  13. JI S, SHANG Z C. The determination of Zn2+ in compound of Zn2+ and Fe2+ by EDTA titration [J]. Shandong Chem Ind, 2000, 29: 40–41. (in Chinese)

    Google Scholar 

  14. KARAMANEV D G, NIKOLOV L N, MAMATARKOVA V. Rapid simultaneous quantitative determination of ferric and ferrous ions in drainage waters and similar solutions [J]. Mineral Engineering, 2002, 15(5): 341–346.

    Article  Google Scholar 

  15. VEGLIÒ F, BEOLCHINI F, NARDINI A, TORO L. Bioleaching of a pyrrhotite ore by a sulfooxidans strains: Kinetic analysis [J]. Chemical Engineering Science, 2000, 55(4): 783–795.

    Article  Google Scholar 

  16. LI Hong-mei. Bioleaching of pyrrhotite bearing nickel—A review [J]. Hydrometallurgy, 1999(3): 8–12. (in Chinese)

  17. FOWLER T A, CRUNDWELL F K. Leaching of zinc sulfide by thiobacillus ferrooxidans: Experiments with a controlled redox potential indicate no direct bacterial mechanism [J]. Appl Environ Microbiol, 1998, 64(10): 3570–3575.

    Google Scholar 

  18. FOWLER T A, CRUNDWELL F K. Leaching of zinc sulfide by thiobacillus ferrooxidans: Bacterial oxidation of the sulfur product layer increases the rate of zinc sulfide dissolution at high concentration of ferrous ions [J]. Appl Environ Microbiol, 1999, 65(12): 5285–5292.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Wen-qing Qin  (覃文庆).

Additional information

Foundation item: Project(50621063) supported by the National Natural Science Foundation of China; Project(2004CD619205) supported by the Major State Basic Research Development Program of China

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, S., Qiu, Gz., Qin, Wq. et al. Bioleaching of sphalerite by Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans cultured in 9K medium modified with pyrrhotite. J. Cent. South Univ. Technol. 15, 503–507 (2008). https://doi.org/10.1007/s11771-008-0095-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11771-008-0095-7

Key words

Navigation