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Sensitive determination of DNA based on resonance light scattering enhancement of azocarmine G and CTAB

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Abstract

A new method for the determination of DNA was developed with azocarmine G(AG) in the presence of cetyltrimethylammonium bromide(CTAB) by the resonance light scattering (RLS) technique. The synthetic samples were determined with satisfactory results, and the reaction mechanism was also studied. The results show that under the optimum conditions, the weak RLS signal of AG can be enhanced by DNA, which results from the formation of a new ternary complex AG-CTAB-DNA with large size. Moreover, the enhanced RLS intensity at 552 nm is directly proportional to the concentration of DNA in the range of 0–1.0 µg/mL for fish sperm DNA (fsDNA) and 0–1.5 µg/mL for calf thymus DNA (ctDNA). Based on this, a new assay of DNA can be established. The detection limits (3σ) are 2.1 ng/mL for fsDNA and 2.2 ng/mL for ctDNA, respectively.

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References

  1. Pasternack R F, Bustamante C, Collings P J, et al. Porphyrin assemblies on DNA as studied by a resonance light scattering technique[J]. Journal of American Chemical Society, 1993, 115: 5393–5399.

    Article  Google Scholar 

  2. Pasternack R F, Schaefer K F, Hambright P. Resonance light scattering studies of porphyrin diacid aggregates[J]. Inorganic Chemistry, 1994, 33: 2062–2065.

    Article  Google Scholar 

  3. Pasternack R F, Collings P J. Resonance light scattering: a new technique for studying chromophore aggregation[J]. Science, 1995, 269: 935–939.

    Article  Google Scholar 

  4. HUANG Cheng-zhi, LI Ke-an, TONG Shen-yang. Determination of nucleic acids by a resonance light-scattering technique with α, β, γ, δ-tetrakis [4-(trimethylammoniumyl) phenyl] porphyrin [J]. Analytical Chemistry, 1996, 68: 2259–2263.

    Article  Google Scholar 

  5. HUANG Cheng-zhi, LI Ke-an, TONG Shen-yang. Determination of nanograms of nucleic acids by their enhancement effect on the resonance light scattering of the cobalt (II)/4-[(5-chloro-2-pyridyl)azo]-1,3-diaminobenzene complex[J]. Analytical Chemistry, 1997, 69: 514–520.

    Article  Google Scholar 

  6. MA Chun-qi, LI Ke-an, TONG Shen-yang. Microdetermination of proteins by resonance light scattering spectro-scopy with bromophenol blue [J]. Analytical Biochemistry, 1996, 239: 86–91.

    Article  Google Scholar 

  7. LIU Shao-pu, LUO Hong-qun, LI Nian-bing, et al. Resonance Rayleigh scattering study of the interaction of heparin with some basic diphenyl naphthylmethane dyes [J]. Analytical Chemistry, 2001, 73: 3907–3914.

    Article  Google Scholar 

  8. FENG Ping, SHU Wei-qun, HUANG Cheng-zhi, et al. Total internal reflected resonance light scattering determination of chlortetracycline in body fluid with the complex cation of chlortetracycline-europium-trioctylphosphine oxide at the water/tetrachloromethane interface [J]. Analytical Chemistry, 2001, 73: 4307–4312.

    Article  Google Scholar 

  9. LIU Shao-pu, HU Xiao-li, LUO Hong-qun. Resonance light scattering measurement of aminoglycoside antibiotics with evans blue[J]. Analytical Sciences, 2003, 19: 927–932.

    Article  Google Scholar 

  10. LIU Shao-pu, LONG Xiu-fen. Recent advances of molecular spectrometric methods for the determination of nucleic acids[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2002, 38 (2): 101–107. (in Chinese)

    Google Scholar 

  11. HUANG Cheng-zhi, LI Yuan-fang, PU Qing-hai, et al. Interactions of Nile blue sulphate with nucleic acids as studied by resonance light scattering measurements and determinations of nucleic acids at nanogram levels[J]. Analytical Letters, 1999, 32: 2395–2415.

    Article  Google Scholar 

  12. HUANG Cheng-zhi, LI Yuan-fang, HUANG Xinhua, et al. Interactions of Janus green B with double stranded DNA and the determination of DNA based on the mea-surement of enhanced resonance light scattering [J]. The Analyst, 2000, 125: 1267–1272.

    Article  Google Scholar 

  13. WANG Min, YANG Jing-he, WU Xia, et al. Study of the interaction of nucleic acids with acridine red and CTMAB by a resonance light scattering technique and determination of nucleic acids at nanogram levels [J]. Analytica Chimica Acta, 2000, 422: 151–158.

    Article  Google Scholar 

  14. LI Yuan-fang, HUANG Cheng-zhi, HUANG Xinhua, et al. Enhanced resonance light scattering of Alcian blue 8GX as an assay of DNA[J]. Analytical Letters, 2001, 34: 1117–1132.

    Article  Google Scholar 

  15. LIU Ru-tao, YANG Jing-he, WU Xia, et al. Interaction of morin-cetyltrimethylammoni umbromide with nucleic acids and determination of nucleic acids at nanograms per milliliter levels based on the enhancement of preresonance light scattering [J]. The Analyst, 2001, 126: 1367–1371.

    Article  Google Scholar 

  16. LI Zheng-ping, LI Ke-an, TONG Shen-yang. Determination for micro amounts of nucleic acids by a resonance light scattering technique with dequalinium chloride[J]. Talanta, 2001, 55: 669–675.

    Article  Google Scholar 

  17. CHEN Zhan-guang, DING Wei-feng, REN Fenglian, et al. Microdetermination of nucleic acids by enhanced resonance light scattering spectroscopy of μ-oxotetraphenyl porphyrinatoiron [J]. Canadian Journal of Analytical Sciences and Spectroscopy, 2005, 50(1): 36–41.

    Google Scholar 

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Central South University, 410083, Changsha, China

    Chen Zhan-guang & Ren Feng-lian (Professor)

  2. Department of Chemistry, Shantou University, 515063, Shantou, China

    Chen Zhan-guang, Liao Xu-hong, Ding Wei-feng & Liu Jin-bin

Authors
  1. Chen Zhan-guang
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  2. Ren Feng-lian
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  3. Si Shi-hui
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  4. Liao Xu-hong
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  5. Ding Wei-feng
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  6. Liu Jin-bin
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Corresponding author

Correspondence to Ren Feng-lian.

Additional information

Foundation item: Project(20475065) supported by the National Natural Science Foundation of China

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Chen, Zg., Ren, Fl., Si, Sh. et al. Sensitive determination of DNA based on resonance light scattering enhancement of azocarmine G and CTAB. J Cent. South Univ. Technol. 12, 688–692 (2005). https://doi.org/10.1007/s11771-005-0070-5

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  • DOI: https://doi.org/10.1007/s11771-005-0070-5

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