Skip to main content
SpringerLink
Log in

Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites

  • Published:
Journal of Central South University of Technology Aims and scope Submit manuscript

Abstract

The solution chemical and optical characteristics of formation of amine-terminated polyamidoamine dendrimer G2.0(NH2-PAMAM G2.0)-Au nanocomposites in the aqueous solution of NH2-PAMAM G2.0 at various mole ratios of Au(III) to NH2-PAMAM G2.0 were studied by both UV-visible spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle is surrounded by several NH2-PAMAM G2.0 dendrimers, emit strong bluish violet fluorescence, and are uniform, water soluble and biocompatible as well as very stable in frozen conditions. The size of gold nanoparticles in the nanocomposites is about 2.5 nm and decreases with the increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2. 0 plays an important role in acting as host or micro-reactor for Au(III) before Au(III) reduction and acting as dispersant and stabilizer for gold nanoparticles after Au(III) reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast cell lines show that the NH2-PAMAM G2.0-Au nanocomposites can be used as optical imaging markers for bioanalyses and medical diagnoses.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Alivisatos A P. Semiconductor clusters, nanocyrstals, and quantum dots [J]. Science, 1996, 271: 933–937.

    Article  Google Scholar 

  2. Warren C W C, Nie S M. Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J]. Science, 1998, 281: 2016–2018.

    Article  Google Scholar 

  3. Taton T A, Mirkin C A, Letsinger R L. Scanometric DNA array detection with nanoparticle probes [J]. Science, 2000, 289: 1757–1760.

    Article  Google Scholar 

  4. Sandhu K K, Mcintosh C M, Simard J M, et al. Gold nanoparticles mediated transfection of mammalian cells [J]. Bioconjug Chem, 2002, 13(1): 3–6.

    Article  Google Scholar 

  5. JIN Rong-chao, WU Guo-sheng, LI Zhi, et al. What controls the melting properties of DNA-Linked gold nanoparticle assemblies? [J]. J Am Chem Soc, 2003, 125(6): 1643–1654.

    Article  Google Scholar 

  6. Liz-Marzan L M, Giersig M, Mulvaney P. Synthesis of nanosized gold-silica core-shell particles[J]. Langmuir, 1996, 12(18): 4329–4335.

    Article  Google Scholar 

  7. Esumi K, Takei N, Yoshimura T. Antioxidant-potentiality of gold-chitosan nanocomposites [J]. Colloids Surf B, 2003, 32(2): 117–123.

    Article  Google Scholar 

  8. Shankar S S, Rai A, Ahmad A, et al. Rapid synthesis of Au, Ag, and bimetallic Au core Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth[J]. J Colloid Interface Sci, 2004, 275(2): 496–502.

    Article  Google Scholar 

  9. Roberts J C, Bhalgat M K, Zera R T. Preliminary biological evaluation of polyamidoamine (PAMAM) Starburst™ dendrimers [J]. J Biomed Mater Res, 1996, 30(1): 53–65.

    Article  Google Scholar 

  10. Torigoe K, Suzuki A, Esumi K. Au (III)-PAMAM interaction and formation of Au-PAMAM nanocomposites in ethyl acetate[J]. J Colloid Interface Sci, 2001, 241(2): 346–356.

    Article  Google Scholar 

  11. Balogh L, Valluzzi R, Laverdure K S, et al. Formation of silver and gold dendrimer nanocomposites[J]. J Nanoparticle Res, 1999, 1(3): 353–368.

    Article  Google Scholar 

  12. Zhao M, Sun L, Crooks R M. Preparation of Cu nanoclusters within dendrimer templates[J]. J Am Chem Soc, 1998, 120(19): 4877–4878.

    Article  Google Scholar 

  13. Esumi K, Isono R, Yoshimura T. Preparation of PAMAM- and PPI-Metal (silver, platinum and palladium) nanocomposites and their catalytic activities for reduction of 4-nitrophenol[J]. Langmuir, 2004, 20(1): 237–243.

    Article  Google Scholar 

  14. Esumi K, Kameo A, Suzuki A, et al. Preparation of gold nanoparticles in formamide and N, N-dimethyl-formamide in the presence of poly(amidoamine) dendrimers with surface methyl ester groups[J]. Colloids Surfaces A, 2001, 189(1–3): 155–161.

    Article  Google Scholar 

  15. Esumil K, Hosoya T, Suzuki A, et al. Preparation of hydrophobically modified poly (amidoamine) dendrimer-encapsulated gold nanoparticles in organic solvents[J]. J Colloid Interface Sci, 2000, 229(1): 303–306.

    Article  Google Scholar 

  16. Esumi K, Miyamoto K, Yoshimura T. Comparison of PAMAM-Au and PPI-Au nanocomposites and their catalytic activity for reduction of 4-nitrophenol[J]. J Colloid Interface Sci, 2002, 254(2): 402–405.

    Article  Google Scholar 

  17. Zheng J, Petty J T, Dickson R M. High quantum yield blue emission from water-soluble Au8 nanodots [J]. J Am Chem Soc, 2003, 125(26): 7780–7781.

    Article  Google Scholar 

  18. Tomalia D A, Baker H, Dewld J, et al. A new class of polymers: starbuest-dendritic macromolecules[J]. Polymer J, 1985, 17(1): 117–132.

    Article  Google Scholar 

  19. SHEN Li, HU Nai-fei. Heme Protein films with polyamidoamine dendrimer: direct electrochemistry and electrocatalysis [J]. Biochim Biophys Acta, 2004, 1608(1): 23–33.

    Article  Google Scholar 

  20. Esumi K, Suzuki A, Yamahira A, et al. Role of poly-(amidoamine) dendrimers for preparing nanoparticles of gold, platinum and silver [J]. Langmuir, 2000, 16(6): 2604–2608.

    Article  Google Scholar 

  21. Huang T, Murray R W. Quenching of [Ru(bpy)3]2+ fluorescence by binding to Au nanoparticles[J]. Langmuir, 2002, 18(18): 7077–7081.

    Article  Google Scholar 

  22. JIANG Zhi-liang, FENG Zhong-wei, LI Yan-sheng, et al. Resonance scattering spectroscopic study of gold nanoparticle[J]. Sci China B, 2001, 31(2): 183–188. (in Chinese)

    MathSciNet  Google Scholar 

  23. West R, Wang Y, Goodson T. Nonlinear absorption properties in novel gold nanostructured topologies [J]. J Phys Chem B, 2003, 107(15): 3419–3426.

    Article  Google Scholar 

  24. Kim Y G, Oh S K, Crooks R M. Preparation and characterization of 1–2 nm dendrimer-encapsulated gold nanoparticles having very narrow size distributions[J]. Chem Mater, 2004, 16(1): 167–172.

    Article  Google Scholar 

  25. Grohn F, Bauer B J, Akpalu Y A, et al. Dendrimer templates for the formation of gold nanoclusters[J]. Macromolecules, 2000, 33(16): 6042–6050.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Resources Processing and Bioengineering, Central South University, 410083, Changsha, China

    Xia Jin-lan (Professor), Fu Jin-dian, Nie Zhen-yuan & Shen li

Authors
  1. Xia Jin-lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Fu Jin-dian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Nie Zhen-yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shen li
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xia Jin-lan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xia, Jl., Fu, Jd., Nie, Zy. et al. Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites. J Cent. South Univ. Technol. 12, 641–646 (2005). https://doi.org/10.1007/s11771-005-0061-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11771-005-0061-6

Key words

CLC number

Search

Navigation