Abstract
Paclitaxel in southern yew tree was quantitatively determined by high performance liquid chromatography (HPLC) with ODS-C18 column. A mixture of CH3OH-H2O-CH3COOH(volume ratio: 55 : 44 : 1) is used as mobile phase and UV detection is carried out at 227 nm, and the column temperature is 20°C. The results show that there is a good linear relationship between the area of paclitaxel and the concentration of the sample in the range 50–500 mg/L for paclitaxel. The corresponding regression equation is Y=13021.7+1.01×106X, r=0.9990. The average recovery is 95.3% and the relative standard deviation is 2.08%.
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TIAN Cheng-guo, WU Jian. A study of taxols of taxus wallichinna zucc [J]. Journal of Gui-zhou Normal University (Natural Science) (in Chinese), 1999, 17(4): 19–20.
Wani M C, Taylor H L, Wall M E, et al. The isolation and structure of taxol, a nonel antileukemic and antitumor agent from taxus previfolia [J]. J Am Chem Soc, 1971, 93: 2325–2332.
CHAN Hai-tao, WEI Zuo-jun, YUAN Ying-jin. Basic study on taxol separation from the organic solvents in two liquid-phase cultures of taxus cells [J]. Chemical Industry and Engineering (in Chinese), 2001, 18(2): 99–102.
YU Guang-ao, MEI Xing-guo, KE Tie. Research on different solvent extraction taxol from taxus Cell [J]. J Huazhong Univ of Sci & Tech (in Chinese), 2000, 28(11): 83–84.
XIAO Guo-yong, LIN Bing-chang, MA Zi-dou. The analysis of extracting process of taxol from the northeastern taxus leaves [J]. Journal of Anshan Institute of I & S Technology (in Chinese), 1999, 22(1): 15–17.
KE Tie, CHEN Jing, ZHOU Ai-wen, et al. Determination of taxol from taxus callus cultures [J]. Biotechnology Information(in Chinese), 2000, (5): 26–29.
ZHANG Zhi-qiang, TIAN Gui-lian, FENG Xiao-li. Studies on initial separation of paclitaxel from the taxus yunnanensis extract [J]. Chinese Journal of Biochemical Pharmaceutics (in Chinese), 1999, 20(2): 58–61.
GUAN Zhi, WANG Ming-yan, SU Jing-yu, et al. Study on chemical composition of taxus chinensis (Pilger) rehd. [J]. Journal of Tropical and Subtropical Botany (in Chinese), 1999, 7(3): 252–254.
HONG Kun, HONG Hua-peng. A Simple Method for Separation of Paclitaxel and Cephalomannine [J]. Journal of Guizhou Normal University (Natural Science) (in Chinese), 2001, 19(3): 99–101.
SHEN Ya-ching, HUANG Meng-qing. Taxane diterpenoids from the root bark of taiwanese yew taxus mairei[J]. Journal of the Chinese Chemical Society (in Chinese), 2000, 47: 1125–1130.
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Foundation item: Project (00KNY1010) supported by the Key Program of Science and Technology Office of Hunan Province
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Shi, Sy., Zhou, cs. Determination of paclitaxel in southern yew tree by HPLC. J Cent. South Univ. Technol. 10, 207–210 (2003). https://doi.org/10.1007/s11771-003-0010-1
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DOI: https://doi.org/10.1007/s11771-003-0010-1