Skip to main content
SpringerLink
Log in

Biocompatibility evaluation in vitro. Part II: Functional expression of human and animal osteoblasts on the biomaterials

  • Published:
Journal of Central South University of Technology Aims and scope Submit manuscript

Abstract

DNA synthesis and collagen formations on the implant material by cell culture in vitro are the most important phenotypical expression to estimate the biocompatibility. In this part, DNA synthesis and collagen formation on implant materials were quantitatively and qualitatively estimated by radioactive isotope H+ -thymidine to incorporate into DNA chains, H+ -proline to incorporate into type I collagen proteins followed by scintillation counting and antibody-antigen immunocytochemistry staining, respectively. Research results demonstrate that hydroxyapatite (HA) stimulates DNA synthesis and collagen formation on the material whereas this stimulation is restricted by adding spinel to the materials. There are statistical differences between the influences of material components on both DNA synthesis and collagen formation. It is supposed that porous materials can supply more platforms for cell anchoring, and more DNA and collagen are synthesised on the porous materials. Immersion in culture medium results in new HA crystal formation on the porous HA materials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Itakura Y, Kasugi A, Sudo H, et al. Development of a new system for evaluating the biocompatibility of implant materials using an osteogenic cell line (MC3T3-E1)[J]. J Biomed Mater Res, 1994, 22: 613–622.

    Article  Google Scholar 

  2. Dubois J C, Exbrayat P, and Couble M L, et al. Effect of new machinable ceramic on behaviour of rat bone cells cultured in vitro[J]. J Biomed Mater Res, 1998, 43: 215–225.

    Article  Google Scholar 

  3. Sautier J M, Nefussi J R, Forest N. In vitro differentiation and mineralization of cartilaginous nodules from enzymatically released rat nasal cartilage cells[J]. Biol Cell, 1993, 78: 181–189.

    Article  Google Scholar 

  4. Van der Rest M. Bone matrix and bone specific products, bone (Vol. 3)[M]. Flowida: CRC Press, 1991, 187–233.

    Google Scholar 

  5. Buckwalter D J A, Cooper R R. Bone structure and function. In international course lectures[J]. The American academy of orthopaedic surgeons, 1987 36: 27–48.

    Google Scholar 

  6. Buckwalter D J A, Glimcher M J, Cooper R R, et al. Bone biology[J]. Bone and Joint Surg, 1995, 77–A(75): 1256–1275.

    Article  Google Scholar 

  7. Bauer G. Osseo-integrated implant (Vol. 1)[M]. Florida: CRC Press, 1990, 81–87.

    Google Scholar 

  8. Hunt J A, Williams D F. Quantifying the tissue response to implanted materials[J]. Biomaterials, 1995, 16: 167–170.

    Article  Google Scholar 

  9. Ruan J, Haung B, Mcgee T D, et al. A study of bioceramic composite materials[J]. Funct Mater, 1993, 24: 261–265.

    Google Scholar 

  10. Wen J, Huang B, Ruan J, et al. Study of bi-active CP-spinel composites[J]. J Central South Inst Min Metallur, 1993, 25: 348–352.

    Google Scholar 

  11. Ishaug S L, Grane G M, Miller M J, et al. Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffold[J]. J Biomed Mater Res, 1997, 36: 17–28.

    Article  Google Scholar 

  12. Bagambisa F B, Joos U. Preliminary studies on the phenomenological behaviour of osteoblasts cultured on hydroxyapatite ceramics[J]. Bimaterials, 1990, 11: 50–56.

    Article  Google Scholar 

  13. Knabe C, Gildenhaar R, Berger G, et al. Morphological evaluation of osteoblasts cultured on different calcium phosphate ceramics[J]. Biomaterials, 1997, 18: 1339–1347.

    Article  Google Scholar 

  14. Groessner S B, Tuan R S. Enhanced extracellular matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro[J]. J Cell Sci, 1992, 101: 209–217.

    Google Scholar 

  15. Carlsson L, Rostlund B, Albrektsson T, et al. Removal torques for polished and rough titanium implants[J]. Int J Oral Maxillofac Implant, 1988, (3): 21–24.

  16. Wilke H J, Claes L, Steineman S. Clinical implant materials: advances in biomaterials, (Vol. 9)[M]. Amsterdam: Elsevier, 1990. 309–314.

    Google Scholar 

  17. Buser D, Schenk R K, Steinemann S, et al. Influence of surface characteristics on bone integration of titanium implants. A histomorphometric study in miniature pigs[J]. J Biomed Mater Res, 1991, 25: 889–902.

    Article  Google Scholar 

  18. Okamoto K, Matsura T, Hoskawa R, et al. RGD peptides regulate the specific adhesion scheme of osteoblasts to hydroxyapatite but not to Titanium[J]. J Dent Res, 1998, 77: 481–478.

    Article  Google Scholar 

  19. Lemons J E. Ceramic: past, present, and future[J]. Bone, 1996, 19: 121–128.

    Article  Google Scholar 

  20. Gregoire M, Orly I, Menanteau J. The influence of calcium phosphate biomaterials on human bone cell activities. An in vitro approach[J]. J Biomed Mater Res, 1990, 24: 165–177.

    Article  Google Scholar 

  21. Nefussi J R, Boylefevre M L, Boilekbache H, et al. Mine-ralisation in vitro of matrix formed by osteoblasts isolated by collagenase digestion[J]. Differentiation, 1985, 29: 160–168.

    Article  Google Scholar 

  22. El-Ghannam A, Ducheyne P, Shapiro I M. Porous bioactive glass and hydroxyapatite ceramic affect bone cell function in vitro along different time lines[J]. J Biomed Mater Res, 1997, 36: 167–180.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory for Powder Metallurgy, Central South University, 410083, Changsha, China

    Ruan Jian-ming

  2. Bioengineering Unit, Strathclyde University, G4 0NW, Glasgow, UK

    Grant M. Helen

Authors
  1. Ruan Jian-ming
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Grant M. Helen
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Foundation item: British Biological Science and Research Council

Biography of the first author: RUAN Jian-ming, doctor of bioengineering, born in 1957, majoring in biomaterials & tissue engineering.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ruan, Jm., Grant, M.H. Biocompatibility evaluation in vitro. Part II: Functional expression of human and animal osteoblasts on the biomaterials. J Cent. South Univ. Technol. 8, 75–82 (2001). https://doi.org/10.1007/s11771-001-0030-7

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11771-001-0030-7

Key word

Search

Navigation