Chinese Geographical Science

, Volume 22, Issue 6, pp 647–658 | Cite as

Responses of soil fauna structure and leaf litter decomposition to effective microorganism treatments in Da Hinggan Mountains, China

  • Meixiang Gao
  • Jingke Li
  • Xueping Zhang


Microorganisms are nutritious resources for various soil fauna. Although soil fauna grazing affects microorganism composition and decomposition rate, the responses of soil fauna and leaf litter decomposition to added microorganism is little understood. In this study, in the coniferous and broad-leaved mixed forest of Tahe County in the northern Da Hinggan Mountains, China, three sampling sites (each has an area of 10 m2) were selected. The first two sites were sprinkled with 250 times (EM1) and 1000 times (EM2) diluted effective microorganism (EM) preparations evenly, and the third site was sprinkled with the same volume of water as a control site. The responses of soil fauna structure and leaf litter decomposition to EM treatment were conducted during three years. The results revealed that EM treatment resulted in significant increase of soil organic matter. The number of soil fauna in the EM1 and EM2 sites increased by 12.88% and 2.23% compared to the control site, and among them springtails and mites showed the highest increase. However, the groups of soil fauna in the EM1 and EM2 sites decreased by 6 and 9, respectively. And the changes in the diversity and evenness index were relatively complicated. EM treatment slowed the decomposition of broad-leaved litter, but accelerated the decomposition of coniferous litter. However, the decomposition rate of broad-leaved litter was still higher than that of coniferous litter. The results of this study suggested that the added microorganisms could help individual growth of soil fauna, and this method led to a change in the process of leaf litter decomposition. This paper did not analyze the activity of soil microorganisms, thus it is difficult to clearly explain the complex relationships among litter type, soil fauna and soil microorganisms. Further research on this subject is needed.


soil fauna leaf litter decomposition effective microorganism treatment Da Hinggan Mountains China 


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  1. Almagro M, Martínez-Mena M, 2012. Exploring short-term leaf-litter decomposition dynamics in a Mediterranean ecosystem: Dependence on litter type and site conditions. Plant and Soil, 358(1–2): 323–335. doi: 10.1007/s11104-012-1187-6CrossRefGoogle Scholar
  2. Balasubramanian E N, Muthukumar M, 2012. Performance of HUASB reactor for treating paper & Pulp wastewater using effective microorganism(EM). International Journal of Engineering Science and Technology, 4(6): 2453–2461.Google Scholar
  3. Bardgett R D, 2005. The Biology of Soil: A community and Ecosystem Approach. Oxford: Oxford University Press.Google Scholar
  4. Boer W, Folman L B, Summerbell R C et al., 2005. Living in a fungal world: Impact of fungi on soil bacterial niche development. FEMS Microbiology Reviews, 29(4): 795–811. doi: 10.1016/j.femsre.2004.11.005CrossRefGoogle Scholar
  5. Bray S R, Kitajima K, Mack M C, 2012. Temporal dynamics of microbial communities on decomposing leaf litter of 10 plant species in relation to decomposition rate. Soil Biology and Biochemistry, 49(6): 30–37. doi: 10.1016/j.soilbio.2012.02.009CrossRefGoogle Scholar
  6. Crowther T W, Boddy L, Jones T H, 2011. Species-specific effects of soil fauna on fungal foraging and decomposition. Oecologia, 167(2): 535–545. doi: 10.1007/s00442-011-2005-1CrossRefGoogle Scholar
  7. Forge T A, Simard S W, 2000. Trophic structure of nematode communities, microbial biomass, and nitrogen mineralization in soils of forests and clearcuts in the southern interior of British Columbia. Canadian Journal of Soil Science, 80(3): 401–410. doi: 10.4141/S99-112CrossRefGoogle Scholar
  8. Gómez-Brandóna M, Lazcano C, Lores M et al., 2010. Detritivorous earthworms modify microbial community structure and accelerate plant residue decomposition. Applied Soil Ecology, 44(3): 237–244. doi: 10.1016/j.apsoil.2009.12.010CrossRefGoogle Scholar
  9. Gómez-Brandón M, Aira M, Lores M et al., 2011a. Changes in microbial community structure and function during vermi-composting of pig slurry. Bioresource Technology, 102(5): 4171–4178. doi: 10.1016/j.biortech.2010.12.057CrossRefGoogle Scholar
  10. Gómez-Brandón M, Aira M, Lores M et al., 2011b. Epigeic earthworms exert a bottleneck effect on microbial communities throughgut associated processes. PLOS ONE, 6(9): 1–9. doi: 10.1371/journal.pone.0024786.CrossRefGoogle Scholar
  11. Gómez-Brandón M, Lores M, Domínguez J, 2012. Species-specific effects of epigeic earthworms on microbial community structure during first stages of decomposition of organic matter. PLOS ONE, 7(2): 1–8. doi: 10.1371/journal.pone.0031895.CrossRefGoogle Scholar
  12. Hasegawa M, 2001. The relationship between the organic matter composition of a forest floor and the structure of a soil arthropod community. European Jounal of Soil Biology, 37(4): 281–284. doi: 10.1016/S1164-5563(01)01099-8.CrossRefGoogle Scholar
  13. Hättenschwiler S, Tiunov A V, Scheu S, 2005. Biodiversity and litter decomposition in terrestrial ecosystems. Annual Review of Ecology, Evolution, and Systematics, 36: 191–218. doi: 10.1146/annurev.ecolsys.36.112904.151932.CrossRefGoogle Scholar
  14. Higa T, Parr J, 1994. Effective microorganisms for sustainable agriculture and healthy environment. Available at:
  15. Hogervorst R F, Dijkhuis M A J, van der Schaar M A et al., 2003. Indications for the tracking of elevated nitrogen levels through the fungal route in a soil food web. Environmental Pollution, 126(2): 257–266. doi: 10.1016/S0269-7491(03)00186-6.CrossRefGoogle Scholar
  16. Huang Z L, Ning P, Liu Z, 2012. Microbial inoculants of environmental material in the compost application research progress. Advanced Materials Research, 534: 230–234. doi: 10.4028/ Scholar
  17. Iwaishi S, 2001. Effect of organic fertilizer and effective microorganisms on growth, yield and quality of paddy-rice varieties. Journal of Crop Production, 3(1): 269–273. doi: 10.1300/J144v03n01_22.CrossRefGoogle Scholar
  18. Jaccard P, 1908. Nouvelles rescherches sur la distribution florale. Bulletin de la Societé Vaudoise des Sciences Natureles, 44: 223–270.Google Scholar
  19. Javaid A, Bajwa R, Anjum T, 2008. Effect of heat-sterilization and EM (effective microorganisms) application on wheat (Triticum aestivum L.) grown in organic-amended sandy loam soil. Cereal Research Communications, 36(3): 489–499. doi: 10.1556/CRC.36.2008.3.13.CrossRefGoogle Scholar
  20. Kampichler C, Rolschewski J, Donnelly D P et al., 2004. Collembolan grazing affects the growth strategy of the cord-forming fungus Hypholoma fasciculare. Soil Biology & Biochemistry, 36(4): 591–599. doi: 10.1016/j.soilbio.2003.12.004.CrossRefGoogle Scholar
  21. Kassa H, Suliman H, Workayew T, 2011. Evaluation of composting process and quality of compost from coffee by-products (Coffee Husk & Pulp). Ethiopian of Journal Environmental Studies and Management, 4(4): 8–13. doi: 10.4314/ejesm.v4i4.2.Google Scholar
  22. Khaliq A, Abbasi M K, Hussain T, 2006. Effects of integrated use of organic and inorganic nutrient sources with effective microorganisms (EM) on seed cotton yield in Pakistan. Bioresource Technology, 97(8): 967–972. doi: 10.1016/j.biortech.2005.05.002.CrossRefGoogle Scholar
  23. Klironomos J N, Bednarczuk E M, Neville J, 1999. Reproductive significance of feeding on saprobic and arbuscular mycorrhizal fungi by the collembolan, Folsomia candida. Functional Ecology, 13(6): 756–761. doi: 10.1046/j.1365-2435.1999.00379.x.CrossRefGoogle Scholar
  24. Leitner S, Wanek W, Wild B et al., 2012. Influence of litter chemistry and stoichiometry on glucan depolymerization during decomposition of beech (Fagus sylvatica L.) litter. Soil Biology & Biochemistry, 50: 174–187. doi: 10.1016/j.soilbio.2012.03.012.CrossRefGoogle Scholar
  25. Lensing J R, Wise D H, 2007. Impact of changes in rainfall amounts predicted by climate-change models on decomposition in a deciduous forest. Applied Soil Ecology, 35(3): 523–534. doi: 10.1016/j.apsoil.2006.09.015.CrossRefGoogle Scholar
  26. Lynd L R, Weimer P J, van Zyl W H et al., 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol and Molecular Biology Reviews, 66(3): 506–577. doi: 10.1128/MMBR.CrossRefGoogle Scholar
  27. Magurran A E, 1988. Ecological Diversity and Its Measurement. Princeton: Princeton University Press.Google Scholar
  28. Olson J S, 1963. Energy storage and the balance of producers and decomposers in ecological systems. Ecology, 44(2): 322–331. doi: 10.2307/1932179.CrossRefGoogle Scholar
  29. Osler G H R, Sommerkorn M, 2007. Toward a complete soil C and N cycle: Incorporating the soil fauna. Ecology, 88(7): 1611–1621. doi: 10.1890/06-1357.1.CrossRefGoogle Scholar
  30. Pansu M, Gautheyrou J, 2006. Handbook of Soil Analysis: Mineralogical, Organic and Inorganic Methods. Heidelberg: Springer-Verlag.Google Scholar
  31. Prescott C E, Zabek L M, Staley C L et al., 2000. Decomposition of broadleaf and needle litter in forests of British Columbia: Influences of litter type, forest type, and litter mixtures. Canadian Journal of Forest Research, 30(11): 1742–1750. doi: 10.1139/x00-097.CrossRefGoogle Scholar
  32. Riutta T, Slade E M, Bebber D P et al., 2012. Experimental evidence for the interacting effects of forest edge, moisture and soil macrofauna on leaf litter decomposition. Soil Biology and Biochemistry, 49: 124–131. doi: 10.1016/j.soilbio.2012.02.028.CrossRefGoogle Scholar
  33. Sangakkara U R, 2012. The technology of Effective microorganisms-Case studies of application. Available at:
  34. Santorufo L, Van Gestel C A M, Rocco A et al., 2012. Soil invertebrates as bioindicators of urban soil quality. Environmental Pollution, 161: 57–63. doi: 10.1016/j.envpol.2011.09.042.CrossRefGoogle Scholar
  35. Seastedt T R, 1984. The role of microarthropods in decomposition and mineralization processes. Annual Review of Entomollogy, 29: 25–46. doi: 10.1146/annurev.en.29.010184.000325CrossRefGoogle Scholar
  36. Tordoff G M, Boddy L, Jones T H, 2008. Species-specific impacts of collembola grazing on fungal foraging ecology. Soil Biology and Biochemistry, 40(2): 434–442. doi: 10.1016/j.soilbio.2007.09.006.CrossRefGoogle Scholar
  37. Van Straalen N M, 1988. Production and biomass turnover in two populations of forest floor Collembola. Netherlands Journal of Zoology, 39(2-3): 156–168. doi: 10.1163/156854289X00093.CrossRefGoogle Scholar
  38. Varga J, Naár Z, Dobolyi C, 2002. Selective feeding of collembolan species Tomocerus longicornis (Müll.) and Orchesella cincta (L.) on moss inhabiting fungi. Pedobiologia, 46(6): 526–538. doi: 10.1078/0031-4056-00157.CrossRefGoogle Scholar
  39. Wardle D A, Bardgett R D, Klironomos J N et al., 2004. Ecological linkages between aboveground and belowground biota. Science, 304(5677): 1629–1633. doi: 10.1126/science.1094875.CrossRefGoogle Scholar
  40. Wood J, Tordoff G M, Jones T H et al., 2006. Reorganization of mycelial networks of Phanerochaete velutina in response to new woody resources and collembola (Folsomia candida) grazing. Mycological Research, 110(8): 985–993. doi: 10.1016/j.mycres.2006.05.013.CrossRefGoogle Scholar
  41. Yeates G W, 2003. Nematodes as soil indicators: Functional and biodiversity aspects. Biology and Fertility of Soils, 37(4): 199–210. doi: 10.1007/s00374-003-0586-5.Google Scholar

Copyright information

© Science Press, Northeast Institute of Geography and Agricultural Ecology, CAS and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Key Laboratory of Remote Sensing Monitoring of Geographic Environment, College of Heilongjiang ProvinceHarbin Normal UniversityHarbinChina
  2. 2.Yecheng Trading Co. Ltd.DandongChina

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