Abstract
We present a simple and general-purpose method able to combine high-resolution procedure with the classification and identification of objects of interest from microscopy imaging. The method is composed of two stages. First (pattern recognition), promising components (possible objects of interest) in the image are detected and small regions containing the objects of interest are extracted using a feature finder. Second, high-resolution algorithms are applied to such identified components in order to approach a multiple scales of resolution. Although the method is indeed to be applied to any microscopy technique, in this paper, we have focused the attention on biological systems, like animal cells, recorded with an atomic force microscopy.
Similar content being viewed by others
References
Gonzalez, R.C., Woods, R.E.: Digital Image Processing, vol. 3. Pearson, Prentice Hall (2008)
Theodorikis, S., Koutroumbas, K.: Pattern Recognition, vol. 4. Academic press, Cambridge (2008)
Bishop, C.M.: Pattern Recognition and Machine Learning. Springer, Berlin (2006)
D’Acunto, M.: Nanotribology and biomaterials: new challenges in atomic force microscopy. In: Gehar, K.S. (ed.) Nanophysics, Nanoclusters and Nanodevices, pp. 1–39. Nova Science publisher, New York (2006)
Kim, K.I., Kwon, Y.: Single-image super-resolution using sparse regression and natural image prior. IEEE Trans. Pattern Anal. Mach. Intell. 32, 1127–1133 (2010)
D’Acunto, M., Pieri, G., Righi, M., Salvetti, O.: A methodological approach for combining super-resolution and pattern-recognition to image identification. Pattern Recognit. Image Anal. 24(2), 209–217 (2014)
Sanchez-Diaz, G., Martinez-Trinidad, J.F.: Determination of similarity threshold in clustering problems for large data sets. Prog. Pattern Recognit. Speech Image Anal. 2905, 611–618 (2003)
Webb, A.R., Copsey, K.D.: Statistical Pattern Recognition. Wiley, New York (2011)
Bunke, H., Kandel, A.: Hybrid methods in pattern recognition. In: Series in Machine Perception and Artificial Intelligence, vol. 47. World Scientific, Singapore (2002)
Goshtasby, A., Shyu, H.-L.: Edge detection by curve fitting. Image Visi. Comput. 13, 169–177 (1995)
Maini, R., Aggarwal, H.: Study and comparison of various image edge detection techniques. Int. J. Image Process. (IJIP) 3, 1–11 (2009)
Dougherty, E.R., Barrera, J.: Pattern recognition theory in nonlinear signal processing. J. Math. Imaging Vis. 16, 181–197 (2002)
Danti, S., D’Acunto, M., Trombi, L., Berrettini, S., Pietrabissa, A.: A micro/nanoscale surface mechanical study on Morpho-functional changes in multilineage-differentiated human mesenchymal stem cells. Macromol. Biosci. 7, 589–598 (2007)
Chacko, J.V., Cella Zanacchi, F., Diaspro, A.: Probing cytoskeletal structures by coupling optical superresolution and AFM techniques for a correlative approach. Cytoskeleton 70(11), 729-40 (2013)
D’Acunto, M., Berrettini, S., Danti, S., Lisanti, M., Petrini, M., Pietrabissa, A., Salvetti, O.: Inferential Mining for Reconstruction of 3D Cell Structures in Atomic Force Microscopy Imaging. In: KDIR-2011, Proceedings of the International Conference on Knowledge Discovery and Information Retrieval, pp. 695–701 (2011)
MacLean, W.J., Tsotsos, J.K.: Fast pattern recognition using normalized grey-scale correlation in a pyramid image representation. Mach. Vis. Appl. 19, 163–179 (2008)
Righi, M.: PRIAR. Technical Report, CNR-ISTI (2014)
Sonka, M., Hlavac, V., Boyle, R.: Image processing, analysis, and machine vision. Thomson Eng (2007)
Ikonen, L., Toivanen, P.: Distance and Nearest Neighbor Transforms of Gray-Level Surfaces Using Priority Pixel Queue Algorithm. Springer, Berlin (2005)
Tian, J., Ma, K.-K.: A survey on super-resolution imaging. Signal Image Video Process. 5(3), 329–342 (2011)
Getreuer, P.: Linear Methods for Image Interpolation. Image Process. On Line (2011). doi:10.5201/ipol.2011.g_lmii
Ardizzone, E., et al.: Fuzzy-based kernel regression approaches for free form deformation and elastic registration of medical images. Biomed. Eng. edt. Carlos Alex. Barros de Mello, pp. 347–368 (2009)
http://www.eecs.berkeley.edu/Research/Projects/CS/vision/bsds/BSDS300/html/dataset/images.html
Gonzalez, R.C., Woods, R.E., Eddins, S.L.: Digital Image Processing Using MATLAB, 2nd edn. Prentice-Hall Inc, Upper Saddle River (2010)
Chan, T.F., Vese, L.A.: Active contours without edges. IEEE Trans. Image Process. 10(2), 266–277 (2001)
Getreuer, P.: Chan–Vese segmentation. Image process. On line 2, 214–224 (2012)
Acknowledgments
The authors like to thank S. Danti for the preparation of hMCSs samples.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that there is no conflict of interest regarding the publication of this manuscript.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
D’Acunto, M., Righi, M. & Salvetti, O. A new method combining enhanced resolution and pattern identification. SIViP 10, 1303–1310 (2016). https://doi.org/10.1007/s11760-016-0947-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11760-016-0947-9