Abstract
Cold-active enzymes are extremozymes produced by psychrophiles, and have attracted much attention as biocatalysts due to their capacity to resist extreme reaction conditions in industrial processes. This study aimed to isolate bacterial strains from Arctic fjord sediments and screen them for lipase enzyme production. Out of 73 isolates, 8 were identified as good lipase producers. Bacillus cereus I13 exhibited the highest activity of 11.42 U/mL on the 4th day of incubation at 20 °C, along with the large zone of clearance of 27 mm. Optimum conditions for lipase production were identified as pH 7.0, a temperature of 20 °C, and 96 h of incubation, with glucose as a carbon source, yeast as a nitrogen source, and olive oil as a substrate. Bacillus cereus I13 has the potential to be used for the industrial production of cold-active lipase, and this study provides evidence that potent isolates can effectively increase lipase production.
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The data supporting the findings of this study are available from the corresponding authors upon reasonable request.
Abbreviations
- BLAST:
-
Basic Local Alignment Search Tool
- CAGR:
-
Compound Annual Growth Rate
- DNA:
-
Deoxyribonucleic acid
- IU:
-
International Unit
- MTCC:
-
Microbial Type Culture Collection
- mM:
-
Millimolar
- PCR:
-
Polymerase Chain Reaction
- ROA:
-
Rhodamine Olive oil Agar
- POA:
-
Phenol red Olive oil Agar
- p-NPP:
-
Para-nitro phenyl palmitate
- rRNA:
-
Ribosomal Ribonucleic acid
- UV:
-
Ultra violet
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Acknowledgment
The first author is grateful to the Council of Scientific and Industrial Research (CSIR) for the awarded fellowship. The authors wish to thank the Department of Marine Biology, Microbiology & Biochemistry, Cochin University of Science and Technology for providing the necessary facilities to carry out the work. The authors are thankful to the Director, National Centre for Antarctic and Ocean Research, Ministry of Earth Sciences, for logistic support and facilities for sample, collection, and analysis.
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The Council of Scientific and Industrial Research (CSIR) supported the first author with grant no.09/239(0534)2018-EMR-1.
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HK designed and performed the experiments, analyzed the data, and prepared the manuscript. VS helped to perform the experiments. SK helped in reviewing and finalizing the manuscript. AP helped to analyze the data and in reviewing the manuscript. KKP helped with logistical support and funding. MH conceptualized the project, designed the experiments, and reviewed and finalized the manuscript. All authors read and approved the final manuscript.
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Kattatheyil, H., Sajeela, V., Kabeer, S.S. et al. Screening, optimization, and molecular characterization of cold-active lipase producing Bacillus cereus I13 from Arctic sediments. Biologia 79, 1041–1055 (2024). https://doi.org/10.1007/s11756-024-01610-y
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DOI: https://doi.org/10.1007/s11756-024-01610-y