Abstract
The success of a breeding program largely depends on the presence of sufficient genetic diversity in crops to provide an avenue for selection of desirable genotypes for utilization in crop improvement. The “wooden box technique” was adopted to evaluate drought tolerance in cowpea landraces cultivated by small-scale farmers in Northern Nigeria. The technique enables high throughput screening of cowpea genotypes for their agro-morphological variability to drought at seedling stage. Therefore, this study aimed to provide data on cowpea landrace responses to drought at the seedling stage, with the objective of helping mitigate crop loss in a region predominantly characterized by early-season drought events. Here, 420 cowpea genotypes (307 landraces, 113 IITA accessions) were screened for tolerance to seedling drought. Time course analysis of growth and agronomic traits revealed gradual cessation of growth as drought stress intensified as evidenced by reduction in trifoliate, increased leaf senescence and stem wilting. Multivariate analysis using principal component (PC) analysis and k-mean clustering identified 3 major clusters where PC1 and PC2 explained 46.7% of the variability in response to drought stress. The biplot analysis showed that plant height, stem greenness and trifoliate contributed positively to PC1 while leaf senescence score was negatively related to the clustering on this axis. The comprehensive data analysis pipeline enabled the identification of the relationship between agronomic and stay-green parameters which provided the understanding of traits that were useful during the selection of landraces under drought stress at the seedling stage. Additionally, our results identified potentially tolerant landraces for drought prone ecologies.
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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.
References
Abebe BK, Alemayehu MT (2022) A review of the nutritional use of cowpea (Vigna unguiculata L. Walp) for human and animal diets. J Agric Food Res 10:100383. https://doi.org/10.1016/j.jafr.2022.100383
Agbicodo EM, Fatokun CA, Muranaka S, Visser RGF, Van Der Linden CG (2009) Breeding drought tolerant cowpea: Constraints, accomplishments, and future prospects. Euphytica 167(3):353–370. https://doi.org/10.1007/s10681-009-9893-8
Ajayi AT, Olumekun VO, Gbadamosi AE (2017) Estimates of genetic variation among drought tolerant traits of cowpea at seedling stage. Int J Plant Res 7(2):48–57. https://doi.org/10.5923/j.plant.20170702.04
Ajayi AT, Gbadamosi AE, Olumekun VO (2018) Screening for drought tolerance in cowpea (Vigna unguiculata L. Walp) at seedling stage under screen house condition. Int J BioSci Technol 11(1):1–19
Aliyu A, Ishiyaku MF, Offei SK, Isaac KA, ElebluAliyu JSYRE (2023) Enhancing cowpea production through breeding efforts for aphid (Aphis crassivora koch) resistance: a review. Euphytica 219:19. https://doi.org/10.1007/s10681-022-03140-7
Bates D, Mächler M, Bolker BM, Walker SC (2015) Fitting linear mixed-effects models using lme4. J Stat Softw 67(1).https://doi.org/10.18637/jss.v067.i01
Belko N, Zaman-Allah M, Cisse N, Diop NN, Zombre G, Ehlers JD, Vadez V (2012) Lower soil moisture threshold for transpiration decline under water deficit correlates with lower canopy conductance and higher transpiration efficiency in drought-tolerant cowpea. Funct Plant Biol 39(4):306–322. https://doi.org/10.1071/FP11282
Boukar O, Belko N, Chamarthi S, Togola A, Batieno J, Owusu E, Haruna M, Diallo S, Umar ML, Olufajo O, Fatokun C (2018) Cowpea (Vigna unguiculata): Genetics, genomics and breeding. Plant Breed 138(4):415–424. https://doi.org/10.1111/pbr.12589
Carvalho M, Castro I, Moutinho-Pereira J, Correia C, Egea-Cortines M, Matos M, Rosa E, Carnide V, Lino-Neto T (2019a) Evaluating stress responses in cowpea under drought stress. J Plant Physiol 241:153001. https://doi.org/10.1016/j.jplph.2019.153001
Carvalho M, Matos M, Castro I, Monteiro E, Rosa E, Lino-Neto T, Carnide V (2019b) Screening of worldwide cowpea collection to drought tolerant at a germination stage. Sci Hortic 247:107–115. https://doi.org/10.1016/j.scienta.2018.11.082
Cui Q, Xiong H, Yufeng Y, Eaton S, Imamura S, Santamaria J, Ravelombola W, Mason RE, Wood L, Mozzoni LA, Shi A (2020) Evaluation of drought tolerance in Arkansas Cowpea lines at seedling stage. HortScience 55(7):1132–1143. https://doi.org/10.21273/HORTSCI15036-20
Daryanto S, Wang L, Jacinthe PA (2015) Global synthesis of drought effects on food legume production. PLoS One 10(6):e0127401. https://doi.org/10.1371/journal.pone.0127401
FAOSTAT (2022) https://www.fao.org/faostat/en/#home Accessed June 11, 2022
Falconer DS, Mackay TFC (1996) Introduction to Quantitative Genetics, 4th edn. Longman Scientific & Technical, Harlow, UK
Galili T (2015) dendextend: An R package for visualizing, adjusting and comparing trees of hierarchical clustering. Bioinformatics 31(22):3718–3720. https://doi.org/10.1093/bioinformatics/btv428
Gu Z, Eils R, Schlesner M (2016) Complex heatmaps reveal patterns and correlations in multidimensional genomic data. Bioinformatics 32(18):2847–2849. https://doi.org/10.1093/bioinformatics/btw313
Harrell F, Dupont C (2021) Package ‘Hmisc’ CRAN- R Package 442. https://cran.r-project.org/web/packages/Hmisc/index.html. Accessed 26 Feb 2022
Horn LN, Nghituwamata SN, Isabella U (2022) Cowpea production challenges and contribution to livelihood in Sub-Saharan region. Agric Sci 13(01):25–32. https://doi.org/10.4236/as.2022.131003
Jayathilake C, Visvanathan R, Deen A, Bangamuwage R, Jayawardana BC, Nammi S, Liyanage R (2018) Cowpea: An overview on its nutritional facts and health benefits: Nutritional and health properties of cowpea. J Sci Food Agric 98(13):4793–4806. https://doi.org/10.1002/jsfa.9074
Kassambara A, Mundt F (2020) Package ‘factoextra.’ CRAN- R Package 84. https://cran.r-project.org/package=factoextra. Accessed 26 Feb 2022
Muchero W, Ehlers JD, Roberts PA (2008) Seedling stage drought-induced phenotypes and drought-responsive genes in diverse cowpea genotypes. Crop Sci 48(2):541–552. https://doi.org/10.2135/cropsci2007.07.0397
Nkomo GV, Sedibe MM, Mofokeng MA (2022) Phenotyping cowpea accessions at the seedling stage for drought tolerance in controlled environments. Open Agriculture 7(1):433–444. https://doi.org/10.1515/opag-2022-0093
Ofori SA, Cobbina SJ, Obiri S (2021) Climate change, land, water, and food security: perspectives from Sub-Saharan Africa. Front Sustain Food Syst 5:1–9. https://doi.org/10.3389/fsufs.2021.680924
Olorunwa OJ, Shi A, Barickman TC (2021) Varying drought stress induces morpho-physiological changes in cowpea (Vigna unguiculata (L.) genotypes inoculated with Bradyrhizobium japonicum. Plant Stress 2:100033. https://doi.org/10.1016/j.stress.2021.100033
Omomowo OI, Babalola OO (2021) Constraints and prospects of improving cowpea productivity to ensure food, nutritional security and environmental sustainability. Front Plant Sci 12. https://doi.org/10.3389/fpls.2021.751731
R Core Team (2013) R: a language and environment for statistical computing. http://www.r-project.org/. Accessed 23 Feb 2022
Ravelombola W, Shi A, Qin J, Weng Y, Bhattarai G, Zia B, Zhou W, Mou B (2018) Investigation on various aboveground traits to identify drought tolerance in cowpea seedlings. HortScience 53(12):1757–1765. https://doi.org/10.21273/HORTSCI13278-18
Ravelombola W, Shi A, Chen S, Xiong H, Yang Y, Cui Q, Olaoye D, Mou B (2020) Evaluation of cowpea for drought tolerance at seedling stage. Euphytica 216(8):1–19. https://doi.org/10.1007/s10681-020-02660-4
Singh BB (2014) Cowpea: The Food Legume of the 21st Century. Crop Science Society of America
Thompson HE, Berrang-Ford L, Ford JD (2010) Climate change and food security in Sub-Saharan Africa: A systematic literature review. Sustainability 2(8):2719–2733. https://doi.org/10.3390/su2082719
Wei T, Simko V (2017) Corrplot. R Package, v. 0.84 – 18. https://cran.r-project.org/web/packages/corrplot/corrplot.pdf. Accessed 26 Feb 2022
Weng Y, Qin J, Eaton S, Yang Y, Ravelombola WS, Shi A (2019) Evaluation of seed protein content in USDA cowpea germplasm. HortScience 54(5):814–817. https://doi.org/10.21273/HORTSCI13929-19
Wickham H, Averick M, Bryan J, Chang W, McGowan L, François R, Grolemund G, Hayes A, Henry L, Hester J, Kuhn M, Pedersen T, Miller E, Bache S, Müller K, Ooms J, Robinson D, Seidel D, Spinu V, Takahashi K, Vaughan D, Wilke C, Woo K, Yutani H (2019) Welcome to the Tidyverse. J Open Source Softw 4(43):1686. https://doi.org/10.21105/joss.01686
Acknowledgements
This research was funded by TETFUND with grant number TETFund/DR&D/CE/NRF/STI/04/VOL1. We also wish to acknowledge undergraduate students of the Departments of Botany and Biology, Ahmadu Bello University, Zaria, for their support during data collection.
Funding
This work was generously supported by TETFUND with grant number TETFund/DR&D/CE/NRF/STI/04/VOL1 awarded to Ramatu Enehezeyi Aliyu.
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All authors contributed to the study conception and design. Material preparation and data collection were performed by SKS and SSI. Data analyses were performed by SKS. The first draft of the manuscript was written by SKS and SSI. All authors commented on previous versions of the manuscript. Draft was reviewed and re-written by REA. All authors read and approved the final manuscript. Funding for the research was obtained by REA.
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Sakariyahu, S.K., Indabo, S.S., Aliyu, A. et al. Cowpea landraces in northern Nigeria: overview of seedling drought tolerance. Biologia 79, 381–392 (2024). https://doi.org/10.1007/s11756-023-01577-2
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DOI: https://doi.org/10.1007/s11756-023-01577-2