Abstract
Lactoferricin is an amphipathic, cationic peptide with antimicrobial properties. This study aimed to investigate the combined effect of chitosan loaded with lactoferricin and antibody against Staphylococcus aureus and Pseudomonas aeruginosa. lactoferricin was prepared using the modified method of Law and Reiter. By injecting two inactivated bacteria S. aureus and P. aeruginosa along with chitosan as an adjuvant to the camel neck, antibodies were prepared and their production was confirmed using the Indirect-ELISA method. Lactoferricin and antibody were loaded on chitosan using thiamine method. The combined effect of lactoferricin and antibody loaded on chitosan against bacteria was investigated using a bacterial growth test in the presence or absence (control) of the sample. Based on the results of statistical analysis, the data obtained from the combined effect of lactoferricin and antibody loaded on chitosan reduced the growth rate of S. aureus and P. aeruginosa compared to the control (without lactoferricin and antibody loaded on chitosan). There is a significant interaction (p ≤ 0.01) between different levels of lactoferricin and antibody loaded on chitosan on the growth of both bacteria. Bacterial growth curves (based on optical density) showed that all concentrations of lactoferricin and antibody dilutions significantly decreased the growth of S. aureus and P. aeruginosa. According to the growth curves, it can be concluded that lactoferricin and antibody loaded on chitosan acted more effectively on gram-positive bacteria than gram-negative bacteria. Based on the results, it can be stated that lactoferricin and antibody loaded on chitosan as an antibacterial can be useful in a variety of commercial programs to control the growth and survival of pathogens.
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Acknowledgements
The support of the Razi Vaccine & Serum Research Institute during this study is appreciated. This manuscript is prepared based on the Ph.D thesis of the first author at Rasht Branch, Islamic Azad University, Rasht, Iran.
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Soleimani, S., Zibaee, S. & Anvari, M. Combined effect of chitosan loaded with lactoferricin and antibody against Staphylococcus aureus and Pseudomonas aeruginosa. Biologia 79, 275–289 (2024). https://doi.org/10.1007/s11756-023-01540-1
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DOI: https://doi.org/10.1007/s11756-023-01540-1