Abstract
Springs serve as a primary water source in most households in the Jammu Himalaya due to the inaccessibility to water from major rivers flowing into deep valleys. However, over the past several decades, many of these springs have started drying up, while many have already suffered degradation. A total of 24 freshwater springs were mapped in the Jammu Himalayan region, out of which seven had dried up permanently. Water quality parameters (pH, temperature, DO, BOD) of the springwater were within the permissible limits for human consumption, but the presence of pathogenic bacteria could pose a health problem to the local population. Construction of concrete walls, dumping of domestic waste and bathing inside the enclosed ponds of springs have blocked the outflow in some springs, which has also resulted in the stagnation and contamination of spring water leading to the growth of pathogenic Gram-negative bacteria like Escherichia coli (E. coli) and some species of Shigella and Salmonella. The 16S rRNA gene was used for the molecular identification of the isolated bacterial species in addition to macro-morphological and biochemical analysis. The results showed the prevalence of indicator bacteria like E. coli, Shigella and Salmonella in the Jammu Himalayan springs at various places, rendering the water unsafe for drinking.
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Data Availability
Data about the 16S rRNA gene sequences have been deposited at NCBI GenBank under the accession numbers: MT102385, MT102386 and MT102387.
Code Availability
Not applicable.
Abbreviations
- AMSL:
-
Above Mean Sea Level
- APHA:
-
American Public Health Association
- BLAST:
-
Basic Local Alignment Search Tool
- BOD:
-
Biological Oxygen Demand
- CFU:
-
Colony Forming Units
- DNA:
-
Deoxyribonucleic Acid
- DO:
-
Dissolved Oxygen
- EMB:
-
Eosin Methylene Blue
- GPS:
-
Global Positioning System
- MEGA:
-
Molecular Evolutionary Genetic Analysis
- NCBI:
-
National Center for Biotechnology Information
- PCR:
-
Polymerase Chain Reaction
- SPSS:
-
Statistical Package for the Social Sciences
- WHO:
-
World Health Organization
References
Altschul SF, Gish W, Miller W et al (1990) Basic local alignment search tool. J Mol Biol 215(3):403410. https://doi.org/10.1016/S0022-2836(05)80360-2
Amrutha K, Patnaik R, Sandeep AS, Pattanaik JK (2023) Climate Change Impact on Major River Basins in the Indian Himalayan Region: Risk Assessment and Sustainable Management. Clim Chang Adapt Risk Manag Sustain Pract Himalaya 45–63. https://doi.org/10.1007/978-3-031-24659-3_3
An YJ, Breindenbach GP (2005) Monitoring E. coli and total coliforms in natural spring water as related to recreational mountain areas. Environ Monit Assess 102(1–3):131–137. https://doi.org/10.1007/s10661-005-4691-9
Andermann C, Longuevergne L, Bonnet S et al (2012) Impact of transient groundwater storage on the discharge of himalayan rivers. Nat Geosci 5(2):127–132. https://doi.org/10.1038/ngeo1356
APHA (2012) Standard methods for the examination of water and wastewater, 22nd edn. American Public Health Association, Washington
Aram SA, Saalidong BM, Osei Lartey P (2021) Comparative assessment of the relationship between coliform bacteria and water geochemistry in surface and ground water systems. PLoS ONE 16(9):e0257715. https://doi.org/10.1371/journal.pone.0257715
Aslam A, Gossman WG (2022) Shigella. StatPearls. StatPearls Publishing
Asoka A, Gleeson T, Wada Y, Mishra V (2017) Relative contribution of monsoon precipitation and pumping to changes in groundwater storage in India. Nat Geosci 10(2):109–117. https://doi.org/10.1038/ngeo2869
Bhat SU, Pandit AK (2010) Limnochemistry of three Freshwater Springs of Kashmir Himalaya. Hydro Nepal: J Water Energy Environ 7(7):54–59. https://doi.org/10.3126/hn.v7i0.4237
Bhat SU, Nisa AU, Sabha I, Mondal NC (2022) Spring water quality assessment of Anantnag district of Kashmir Himalaya: towards understanding the looming threats to spring ecosystem services. Appl Water Sci 12. https://doi.org/10.1007/S13201-022-01691-7
BIS (2012) I.S.D.W.S. Bureau of Indian Standards, New Delhi, pp 2–3
Cabral JPS (2010) Water Microbiology Bacterial Pathogens and Water. Int J Environ Res Pub Health 7(10):3657. https://doi.org/10.3390/IJERPH7103657
CGWB (2018) Guide on artificial recharge to groundwater. Central Groundwater Board, Ministry of Water
Chand D, Lata R, Dhiman R, Kumar K (2023) Groundwater potential Assessment using an Integrated AHP-Driven geospatial techniques in the High-Altitude Springs of Northwestern Himalaya, India. Clim Chang Adapt Risk Manag Sustain Pract Himalaya 337–360. https://doi.org/10.1007/978-3-031-24659-3_15
Chattopadhyay R, Chakraborty S, Sahai AK (2018) Impact of climatic stress on groundwater resources in the coming decades over South Asia. Groundw Dev Manag Issues Challenges South Asia 397–420. https://doi.org/10.1007/978-3-319-75115-3_17
Daniel D, Anandhi A, Sen S et al (2021) Conceptual Model for the Vulnerability Assessment of Springs in the Indian Himalayas. Clim 2021, Vol 9, Page 121 9:121. https://doi.org/10.3390/CLI9080121
Dass B, Abhishek, Sen S et al (2021) Assessment of spring flows in Indian Himalayan micro-watersheds – a hydro-geological approach. J Hydrol 598:126354. https://doi.org/10.1016/J.JHYDROL.2021.126354
Farooq S, Nazir R, Ganai SA, Ganai BA (2021) Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of western himalayan glacial soil. Sci Rep 2021 111 11:1–14. https://doi.org/10.1038/s41598-021-92197-w
Ghimire M, Chapagain PS, Shrestha S (2019) Mapping of groundwater spring potential zone using geospatial techniques in the Central Nepal Himalayas: a case example of Melamchi–Larke area. J Earth System Science 128(2):1–24. https://doi.org/10.1007/S12040-018-1048-7
He Q, Kuang X, Chen J et al (2023) Glacier retreat and its impact on groundwater system evolution in the Yarlung Zangbo source region, Tibetan Plateau. J Hydrol Reg Stud 47:101368. https://doi.org/10.1016/J.EJRH.2023.101368
Hiraishi A, Saheki K, Horie S (1984) Relationships of total coliform, fecal coliform, and Organic Pollution levels in the Tamagawa River. Nippon Suisan Gakkaishi 50(6):991–997. https://doi.org/10.2331/suisan.50.991
Jasrotia AS, Singh R (2007) Hydrochemistry and groundwater quality, around Devak and Rui watersheds of Jammu region, Jammu and Kashmir. J Geol Soc India 69(5):1042–1054
Jasrotia AS, Majhi A, Singh S (2009) Water balance approach for rainwater harvesting using remote sensing and GIS techniques, Jammu Himalaya, India. Water Resour Manag 23(14):3035–3055. https://doi.org/10.1007/s11269-009-9422-5
Jeelani G, Shah RA, Fryar AE et al (2018) Hydrological processes in glacierized high-altitude basins of the western Himalayas. Hydrogeol J 26:615–628. https://doi.org/10.1007/s10040-017-1666-1
Khair SM, Mushtaq S, Reardon-Smith K (2015) Groundwater Governance in a water-starved country: Public Policy, Farmers’ perceptions, and drivers of Tubewell Adoption in Balochistan. Pakistan Groundw 53(4):626–637. https://doi.org/10.1111/gwat.12250
Kiwanuka M, Mutanda HE, Niyomukiza JB, Nakasagga E (2023) Assessment of suitability of drinking water from the springs in Urban slums of Kampala. Environ Challenges 10:100667. https://doi.org/10.1016/J.ENVC.2022.100667
Kumar V, Sen S (2018) Evaluation of spring discharge dynamics using recession curve analysis: a case study in data-scarce region, lesser Himalayas, India. Sustain Water Resour Manag 4:539–557. https://doi.org/10.1007/S40899-017-0138-Z
Kumar K, Rawat DS, Joshi R (1997) Chemistry of springwater in Almora, Central Himalaya, India. Environ Geol 31(3–4):150–156. https://doi.org/10.1007/s002540050174
Kumar M, Rathod R, Mukherji A (2023) Water Security and Spring Conservation in the Himalaya. 15–36. https://doi.org/10.1007/978-3-031-16648-8_2
Llamas MR, Martínez-Santos P (2005) Intensive Groundwater Use: Silent Revolution and potential source of social conflicts. J Water Resour Plan Manag 131(5):337–341
Lone SA, Jeelani G, Deshpande RD et al (2023) Assessing the hydrological controls on spatio-temporal patterns of streamwater in glacierized mountainous Upper Indus River Basin (UIRB), western Himalayas. J Hydrol 619:129310. https://doi.org/10.1016/J.JHYDROL.2023.129310
Lozano R, Naghavi M, Foreman K et al (2012) Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the global burden of Disease Study 2010. The Lancet 380(9859):2095–2128. https://doi.org/10.1016/S0140-6736(12)61728-0
Mishra AK (2023) Threats to mountainous soils: conservation and management strategies. Underst Soils Mt Landscapes 23–39. https://doi.org/10.1016/B978-0-323-95925-4.00007-8
Molden DJ, Vaidya RA, Shrestha AB, Shrestha MS (2014) Water infrastructure for the Hindu Kush Himalayas. Int J Water Resour Dev 30:60–77. https://doi.org/10.1080/07900627.2013.859044
Moiwo JP, Yang Y, Tao F et al (2011) Water storage change in the Himalayas from the gravity recovery and climate experiment (GRACE) and an empirical climate model. Water Resour Res 47. https://doi.org/10.1029/2010WR010157
Momtaz H, Dehkordi FS, Rahimi E, Asgarifar A (2013) Detection of Escherichia coli, Salmonella species, and Vibrio cholerae in tap water and bottled drinking water in Isfahan, Iran. BMC Public Health 13. https://doi.org/10.1186/1471-2458-13-556
Nisa FU, Umar R (2023) Evaluation of physicochemical and microbiological parameters, and their correlation in Himalayan Spring Water Systems: a case study of District Kulgam of Kashmir Valley, India, Western Himalaya. Environ Monit Assess 195:1–19. https://doi.org/10.1007/S10661-023-11025-y
Panwar S (2020) Vulnerability of Himalayan springs to climate change and anthropogenic impact: a review. J Mt Sci 17:117–132 (2020). https://doi.org/10.1007/s11629-018-5308-4
Paramanik SK (2017) Analysis of discharge variation and estimation of recession coefficients for different spring systems in himalayan terrain. Indian Institute of Technology Roorkee: Master Degree
Poudel DD, Duex TW (2017) Vanishing springs in nepalese mountains: Assessment of water sources, farmers’s perceptions, and climate change adaptation. Mt Res Dev 37(1):35–46. https://doi.org/10.1659/MRD-JOURNAL-D-16-00039.1
Prakash A, Molden D (2020) Water in Himalayan Towns: Lessons for Adaptive Water Governance. IWA Publishing. https://doi.org/10.2166/9781789061901
Rana S, Gupta V (2009) Watershed Management in the Indian Himalayan Region: Issues and Challenges. World Environmental and Water Resources Congress 2009. American Society of Civil Engineers, Reston, VA, pp 1–12. https://doi.org/10.1061/41036(342)527
Rodell M, Velicogna I, Famiglietti JS (2009) Satellite-based estimates of groundwater depletion in India. Nature 460(7258):999–1002. https://doi.org/10.1038/nature08238
Sambrook J, Russell DW (2006) Agarose gel electrophoresis. CSH Protoc 1. https://doi.org/10.1101/pdb.prot4020
Scott CA, Zhang F, Mukherji A et al (2019) Water in the Hindu Kush Himalaya, in: Hindu Kush Himalaya Assess, Springer Int Pub, pp 257–299. https://doi.org/10.1007/978-3-319-92288-1_8
Sharma B, Nepal S, Gyawali D et al (2016) Springs, storage towers, and water conservation in the midhills of Nepal. Nepal Water Conservation Foundation and International Center for Mountain Development. ICIMOD Working Paper 2016/3. Kathmandu: Nepal
Singh AK, Das S, Singh S et al (2019) Physicochemical parameters and alarming coliform count of the potable water of Eastern Himalayan state Sikkim: an indication of severe fecal contamination and immediate health risk. Front Cell Dev Biol 7:174. https://doi.org/10.3389/FPUBH.2019.00174
Stange C, Tiehm A (2020) Occurrence of antibiotic resistance genes and microbial source tracking markers in the water of a karst spring in Germany. Sci Total Environ 742:140529. https://doi.org/10.1016/j.scitotenv.2020.140529
Stocker TF, Qin D, Plattner GK, Tignor MMB, Allen SK, Boschung J, Nauels A, Xia Y, Bex V, Midgley PM (2013) Climate change 2013 the physical science basis: Working Group I contribution to the fifth assessment report of the intergovernmental panel on climate change. Cambridge University Press, pp 1–1535. https://doi.org/10.1017/CBO9781107415324
Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol 38:3022–3027. https://doi.org/10.1093/MOLBEV/MSAB120
Taylor RG, Scanlon B, Döll P et al (2013) Ground water and climate change. Nat Clim Change 3:322–329. https://doi.org/10.1038/nclimate1744
Tiwari PC, Joshi B (2012) Environmental changes and sustainable development of water resources in the himalayan headwaters of India. Water Resour Manag 26(4):883–907. https://doi.org/10.1007/s11269-011-9825-y
WHO (2018) Guidelines for drinking-water quality, 3rd edn. Geneva, Switzerland
Zhang X, Liu J, Zhao X et al (2019) Linking physical water consumption with virtual water consumption: methodology, application and implications. J Clean Prod 228:1206–1217. https://doi.org/10.1016/j.jclepro.2019.04.297
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The authors would like to thank the NMHS, MoEF&CC, Government of India for providing the necessary funding to carry out the research work.
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SF did sampling, collected, compiled and interpreted the data. Further, conducted experiments, wrote the manuscript and designed the figures. IR designed the research work, evaluated the data and did revision of the manuscript. RN did the critical revision of the manuscript. GJD helped in statistical analysis.
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Farooq, S., Nazir, R., Rashid, I. et al. Microbial pathogen profiling and water quality assessment of Jammu Himalayan springs. Biologia 78, 3679–3690 (2023). https://doi.org/10.1007/s11756-023-01527-y
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DOI: https://doi.org/10.1007/s11756-023-01527-y