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Morphological and molecular observations of nematodes belonging to the family Hoplolaimidae from the Thar Desert, Pakistan

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Abstract

The populations of ectoparasitic nematodes belonging to the family Hoplolaimidae viz., Helicotylencshus dihystera, H. exallus, H. pseudorobustus, and Hoplolaimus seinhorsti were recovered from the soil surrounding the roots of khejri tree (Prosopis cineraria) and Leptadenia pyrotechnica during summer at high temperature (max 50 °C) from the Thar Desert, near Umerkot, Sindh, Pakistan. The species were identified on the basis of morphological and molecular characteristics. According to morphology and morphometrics, these species agree with those of the type species and topotype specimens. The phylogenetic analysis of Helicotylenchus species based on the 18S rDNA placed the studied population in the same clade with other Helicotylenchus with a 1.00 posterior probability. Additionally, phylogenetic analysis of H. seinhorsti using 18S rDNA and cox1 mtDNA sequences placed the Pakistani population close to H. columbus and H. indicus, however, in a different clade with 1.00 posterior probability support value. Measurements, illustrations, LM pictures, and molecular characters of 18S rDNA, and cox1 mtDNA are given. In conclusion, despite the confirmation of the studied species, their ecological role in the agricultural field of Pakistan should be investigated.

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Acknowledgements

The authors thank Dr. Firoza Kazi for providing literature.

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Authors and Affiliations

  1. National Nematological Research Centre, University of Karachi, Karachi, 75270, Pakistan

    Ramzan Ali, Erum Iqbal & Saboohi Raza

  2. Department of Research Administration and Development, University of Limpopo, Private Bag X1106, Sovenga, 0727, Polokwane, South Africa

    Ebrahim Shokoohi

  3. Pakistan Museum of Natural History, Islamabad, Pakistan

    Muhammad Ismail Bhatti

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  1. Ramzan Ali
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Correspondence to Erum Iqbal.

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Ali, R., Shokoohi, E., Iqbal, E. et al. Morphological and molecular observations of nematodes belonging to the family Hoplolaimidae from the Thar Desert, Pakistan. Biologia 78, 3463–3477 (2023). https://doi.org/10.1007/s11756-023-01483-7

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