Abstract
The objective of the present experiment was to investigate the effect of a chelated form of trace minerals in the diet on hatchability, immune system response, and blood parameters in Ross 308 broiler breeders. In this experiment, 560 broiler breeder chickens (520 females and 40 males) of Ross 308 with an average age of 52 weeks were used based on a completely randomized design. There were eight treatments with five replicates and in each replicate there were 13 hens and one rooster. The experimental diets were the same except for the form of the trace minerals. Treatment 1 (negative control): Mn, Se, Cu, Fe and Zn provided as non-chelated form; treatment 2 (positive control): Mn, Se, Cu, Fe and Zn provided as chelated form; treatment 3: Mn, Se, Cu, Fe and Zn provided as 50% chelated form and 50% non-chelated form; treatment 4: Zn provided as chelated form; treatment 5: Cu provided as chelated form; treatment 6: Fe provided as chelated form; treatment 7: Mn provided as chelated form; treatment 8: Se provided as chelated form. In all treatment groups, the quantities of Zn, Cu, Fe, Mn, Se and I were added as much as 110, 10, 50, 120, 0.3 and 2 mg kg− 1 feed based on their requirements, respectively. Data on hatchability, immune system response, and blood parameters were analyzed using SPSS software. The results showed that the number and percentage of hatched chicks were affected by treatment throughout experimental period (p < 0.05), such that treatments 2 and 3 had the highest values compared with the other treatments (p < 0.05); however, the number and percentage of unhatched chicks were not affected by treatment. The number and percentage of fertilized eggs throughout the production period were not affected by the treatments. Hatch residue analysis revealed that the percentage of mild embryonic mortality throughout the production period was affected by the experimental treatments (p < 0.05), with the lowest rate in treatment 2. In addition, no significant difference was found between the experimental treatments in terms of early embryonic mortality, late embryonic mortality, contaminated, external pip, and cracked throughout the production period. The results of the immune system examination and blood parameters showed that treatments 2 and 3 had the highest antibody titers against Newcastle (p < 0.05). In contrast to the levels of low-density lipoproteins (LDL) and high-density lipoprotein (HDL), the levels of estrogen and cholesterol were affected by the experimental treatments (p < 0.05) and there was a significant difference between the experimental treatments in the level of liver enzyme alkaline phosphatase (p < 0.05). The research results indicate an improvement in the hatchability and immune system of broilers fed experimental diets containing chelated forms of Zn, Fe, Cu, Se, and Mn (treatment 2) and a basal diet containing 50% of the minerals Zn, Fe, Cu, Se, and Mn in the sulfate form and 50% in the chelate form (treatment 3).
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Raw data is available from corresponding author upon reasonable request.
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This manuscript is prepared based on Ph.D. thesis of the first author at Rasht Branch, Islamic Azad University, Rasht, Iran. Financial support by Rasht Branch, Islamic Azad University, grant number 17.16.1.462 is gratefully acknowledged.
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Faghih-Mohammadi, F., Seidavi, A. & Bouyeh, M. Effects of the dietary chelated form of trace minerals on hatchability, immune system response, blood constitutes, and estrogen hormone in Ross 308 broiler breeders. Biologia 78, 3427–3439 (2023). https://doi.org/10.1007/s11756-023-01480-w
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DOI: https://doi.org/10.1007/s11756-023-01480-w