Abstract
Soil is one of the great reservoirs of microorganisms. A multiple-metal resistant Pseudomonas aeruginosa strain isolated from arable soil was screened for lipase activity since it plays a crucial role in bacterial cellular metabolism. This strain showed higher minimum inhibitory concentrations for the metal Ni, Zn and Cd. The effect of these heavy metals on lipase production was studied by examining qualitative and quantitative aspects of lipase activity in tributyrin agar base medium and lipase production media, respectively. The lipase activity was found to be 304.45 U/L in the control group (without heavy metals), which was higher than in the groups treated with heavy metals. A statistically significant difference was detected in the metal-induced alteration of the lipolytic activity of the studied strain. The inhibitory effect of the heavy metals on lipase production was assessed, with Zn showing the highest inhibition, while Ni exhibited the lowest inhibitory effect among the three metals studied. The lipolytic activity of the strain was altered by the heavy metals, as demonstrated by ATR-FTIR analysis that revealed changes in the functional groups involved in lipase production under heavy metal stress compared to the control. This study is the first attempt to investigate the extracellular lipase activity of a multiple heavy metal-resistant Pseudomonas strain isolated from arable soil of Uttar Dinajpur region of West Bengal, India, which requires further research for future utilization.
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Abbreviations
- FTIR:
-
Fourier transform infrared spectroscopy
- MIC:
-
Minimum inhibitory concentration
- TBA:
-
Tributyrin agar
- ROA:
-
Rhodamine olive oil agar
- PM I:
-
Production medium I
- PM II:
-
Production medium II
- TPH:
-
Thymolphthalein
- MTC:
-
Maximum tolerable concentration
- ATR-FTIR:
-
Attenuated total reflectance - Fourier transform infrared spectroscopy
- ANOVA:
-
Analysis of variance
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The authors would like to acknowledge DST-SAIF, IIT Madras for their sophisticated analytical services (ATR-FTIR).
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JS: Conceptualization, Methodology, Investigation, Original draft writing, Data acquisition and analysis; MGC: Investigation, experimentation, initial draft writing, Data analysis; SK: Methodology, Investigation, Data collection and manipulation; AC: Conceptualization, Methodology, Review, Visualization; AP: Conceptualization, Supervision, Investigation, Methodology, Writing – Original Draft, Review & Editing, Validation, Visualization, Resources.
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Saha, J., Chaki, M.G., Karmakar, S. et al. Effect of different heavy metals on lipase production by a multiple heavy metal-resistant Pseudomonas aeruginosa strain isolated from arable land. Biologia 78, 2975–2985 (2023). https://doi.org/10.1007/s11756-023-01465-9
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DOI: https://doi.org/10.1007/s11756-023-01465-9