Abstract
This study describes the development of iron manganese doped graphene quantum dots (FeMnO4@GQD) having peroxidase-like catalytic activity detected through chromogenic reaction in the presence of supplementary H2O2. Synthesized hetero-atoms doped GQD showed excellent peroxidase mimicking activity with a Vmax value of 9.91547 × 10− 6 (M/min) and Km value of 0.0491 mM towards substrate TMB (based on Hanes-Woolf kinetics) suggesting higher affinity towards the substrate than the natural enzyme (horseradish peroxidase). In another experiment, we systemically examined the efficacy of GQD and its nanocomposites on germination, vigor, and biochemical activity of bread wheat (Triticum aestivum). Prepared nanomaterials were applied at 200 and 500 µg/mL, initially through seed priming (solid matrix priming) and later through foliar spray on transplanted wheat seedlings grown on nutrient-free sand. The application of iron manganese nanocomposites (FeMnO4) and FeMnO4@GQD improve seed germination and maintain an optimum level of carbohydrates and protein contents that eventually promotes seedling growth. Different germination-related parameters were found to be influenced by the applied higher dose (500 µg/mL) of FeMnO4@GQD. Seedling growth, plant biomass, and biochemical parameters like chlorophyll, carotenoid, sugar, protein, and phenolic content were stimulated by both FeMnO4 and FeMnO4@GQD applications.
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The author would like to thank Department of Chemistry and University Science Instrumentation Centre (USIC), University of North Bengal for providing instrumental facility.
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Md Salman Haydar: Conceptualization, Investigation, Methodolgy, Data Curation, Validation, Software, Formal analysis, Writing-original draft manuscript; Salim Ali: Resources, Methodology, Software, Data curation, Validation; Writing; Palash Mandal: Resources, Methodolgy, Supervision, Writing- Reviewing and Editing; Debadrita Roy: Investigation, Methodology, Formal analysis, Software; Mahendra Nath Roy: Resources, Supervision, Methodology, Investigation; Sourav Kundu: Investigation, Formal analysis; Sudipta Kundu: Software, Data Curation; Chandrani Choudhuri: Conceptualization, Resources, Methodolgy, Validation, Supervision, Writing- Reviewing, Editing and Finalization.
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Haydar, M.S., Ali, S., Mandal, P. et al. Iron-manganese nanocomposites doped graphene quantum dots as growth promoter of wheat and its biomimetic activity. Biologia 78, 2701–2716 (2023). https://doi.org/10.1007/s11756-023-01455-x
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DOI: https://doi.org/10.1007/s11756-023-01455-x